A5027190310- Cell Adhesion Molecules Research
- Social and Educational Sciences
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Protein Tyrosine Phosphatases
- Immune Response and Inflammation
- Chronic Lymphocytic Leukemia Research
- Blood disorders and treatments
- Caveolin-1 and cellular processes
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Analytical Chemistry and Chromatography
- Phagocytosis and Immune Regulation
- Cancer Cells and Metastasis
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Biochemical and Structural Characterization
- Signaling Pathways in Disease
- PI3K/AKT/mTOR signaling in cancer
- Information Technology Governance and Strategy
- Connexins and lens biology
- Microfluidic and Bio-sensing Technologies
Umeå University
2025
Lund University
2003-2020
Örebro University
2017-2020
County Administrative Board
2018
Cancer Research UK
2007-2013
King's College School
2012
King's College London
2011-2012
The Honourable Society of Lincoln's Inn
2008-2009
Pfizer (United States)
1996
Uppsala University
1994
The role of exosomes in cancer can be inferred from the observation that they transfer tumor cell derived genetic material and signaling proteins, resulting e.g. increased angiogenesis metastasis. However, membrane transport mechanisms events involved uptake these virus-like particles remain ill-defined. We now report internalization glioblastoma (GBM) cells involves nonclassical, lipid raft-dependent endocytosis. Importantly, we show raft-associated protein caveolin-1 (CAV1), analogy with...
Loss of the phosphatase PTPN22 enhances functions both effector and regulatory T cells.
The immune cells named T lymphocytes circulate around the body fulfilling their role in immunosurveillance by monitoring tissues for injury or infection. To migrate from blood into tissues, they make use of integrin LFA-1 which is exclusively expressed cells. These highly motile attach and on substrates expressing ligand ICAM-1. molecular events signaling activation adhesion are now reasonably well identified, but process detaching adhesions less understood. cysteine protease calpain...
Abstract γδ T cells have been attributed a wide variety of functions, which in some cases may appear as contradictory. To better understand the enigmatic biology it is crucial to define constituting subpopulations. previously categorized into two subpopulations: CD8αα+ and CD8− cells. In this study we defined characterized novel subset human T-cells expressing CD8αβ. These CD8αβ+ differed from described cell subsets several aspects, including degree enrichment within gut mucosa, activation...
Isotope dilution/mass spectrometric methods for total serum cholesterol, developed separately at the Karolinska Institutet (KI) and National Bureau of Standards (NBS), were compared by applying them to a common set pools. A search cause systematic difference few percent in results from two revealed that KI cholesterol standard contained lathosterol, which interfered with calibration method. With NBS Standard Reference Material used new analyses KI, average mean values dropped 0.2%. The are...
Integrins are heterodimeric transmembrane proteins that play a fundamental role in the migration of leukocytes to sites infection or injury. We found protein tyrosine phosphatase nonreceptor type 22 (PTPN22) inhibits signaling by integrin lymphocyte function-associated antigen-1 (LFA-1) effector T cells. PTPN22 colocalized with its substrates at leading edge cells migrating on surfaces coated LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). Knockout knockdown expression autoimmune...
Between June-September 2018, 20 hepatitis A cases were notified in six counties Sweden. Combined epidemiological and microbiological investigations identified imported frozen strawberries produced Poland as the source of outbreak. Sequence analysis confirmed outbreak strain IB with 100 % identity respective batch was withdrawn. Sharing sequence information internationally led to identification 14 additional Austria, linked from same producer.
Serum from patients was pooled, filtered, dispensed, and frozen. This pooled specimen used for accuracy control in 64 participating laboratories Sweden. Mean values ("state-of-the-art" values) were obtained creatinine, cholesterol, glucose, urea, uric acid, cortisol. These compared with highly accurate reference methods based on isotope dilution-mass spectrometry. Differences marked the case of determination creatinine Concerning other components, differences between state-of-the-art value...
Aortic smooth muscle cells produce chondroitin/dermatan sulfate (CS/DS) proteoglycans that regulate extracellular matrix organization and cell behavior in normal pathological conditions. A unique feature of CS/DS is the presence iduronic acid (IdoA), catalyzed by two DS epimerases. Functional ablation DS-epi1, main epimerase these cells, resulted a major reduction IdoA both on surface secreted proteoglycans. Downregulation led to delayed ability re-populate wounded areas due loss directional...
Imaging analysis reveals a mechanism for the integrin recycling required to maintain T cell migration.
Abstract Leukocyte adhesion deficiency‐III (LAD‐III) is a rare disorder characterized by abnormal signaling to β integrins, leading defective leukocyte and chemotaxis platelet aggregation. Here we present the first case of an African‐American female infant with this disorder. She had history multiple infections, bleeding, leukocytosis since birth. was successfully treated allogeneic bone marrow transplant using reduced intensity‐conditioning regimen. Mutations in KINDLIN‐3 have been...
The role of Gαi proteins coupled to chemokine receptors in directed migration immune cells is well understood. In this study we show that the separate class Gαq/11 required for underlying ability T migrate both randomly and a chemokine-dependent manner. Interfering with Gαq or Gα11 using dominant negative cDNA constructs siRNA causes accumulation LFA-1 adhesions stalled migration. has an impact on expression at plasma membrane level also its internalization. Additionally co-localizes LFA-1-...
Abstract A new UV‐absorption detector cell for on‐column detection in open tubular column liquid chromatography was evaluated. Optical fibers and quick‐fit connections were employed flexibility ease alignment mounting an oven high temperature detection. Different optical fiber diameters evaluated 50 μm i.d. capillary columns. The best combination of (200 source collector) resulted a limit 2 × 10 −6 M acetophenone. No dramatic change the signal‐to‐noise ratio found when raised to 150°C.
The affinity of T-cell receptors (TCRs) for major histocompatibility complex molecules (MHCs) presenting cognate antigens likely determines whether T cells initiate immune responses, or not. There exist few measurements two-dimensional (2D) TCR-MHC interactions, and the effect auxiliary proteins on binding is unexplored. Here, Jurkat T-cells expressing MHC molecule HLA-DQ8-glia-α1 ligand an adhesion protein (rat CD2) were allowed to bind supported lipid bilayers (SLBs) fluorescently labelled...
LPS-activated murine peritoneal macrophages produce IL-1 beta but externalize little mature cytokine in the absence of a secondary stimulus, and CTLs previously were reported to serve this capacity. The release 17-kDa from BALB/c occurred rapidly after addition C57/B1-derived allogeneic CTLs; within 30 min coculture, was observed medium, maximum achieved 4 h. CTL-induced post-translational processing efficient, >80% newly synthesized pro-IL-1 released into medium as species. Externalization...