A5011477619- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Inflammatory Bowel Disease
- Pharmacological Effects of Natural Compounds
- Plant Micronutrient Interactions and Effects
- Epigenetics and DNA Methylation
- Animal Diversity and Health Studies
- Microscopic Colitis
- Chemokine receptors and signaling
- Hepatitis C virus research
- Urological Disorders and Treatments
- Mast cells and histamine
- Calcium signaling and nucleotide metabolism
- Advances in Cucurbitaceae Research
- Hepatitis B Virus Studies
- Identification and Quantification in Food
- Histone Deacetylase Inhibitors Research
- Cancer, Stress, Anesthesia, and Immune Response
- Intestinal Malrotation and Obstruction Disorders
- Gastrointestinal disorders and treatments
- Food Allergy and Anaphylaxis Research
Technical University of Denmark
2020-2025
Stanford University
2023
Lund University
2016-2023
Abstract Neoantigen vaccines are under investigation for various cancers, including epidermal growth factor receptor ( EGFR )-driven lung cancers 1,2 . We tracked the phylogenetic history of an mutant cancer treated with erlotinib, osimertinib, radiotherapy and a personalized neopeptide vaccine (NPV) targeting ten somatic mutations, exon 19 deletion (ex19del). The ex19del mutation was clonal, but is likely to have appeared after whole-genome doubling (WGD) event. Following osimertinib NPV...
Abstract γδ T cells have been attributed a wide variety of functions, which in some cases may appear as contradictory. To better understand the enigmatic biology it is crucial to define constituting subpopulations. previously categorized into two subpopulations: CD8αα+ and CD8− cells. In this study we defined characterized novel subset human T-cells expressing CD8αβ. These CD8αβ+ differed from described cell subsets several aspects, including degree enrichment within gut mucosa, activation...
The majority of neoantigens arise from unique mutations that are not shared between individual patients, making neoantigen-directed immunotherapy a fully personalized treatment approach. Novel technical advances in next-generation sequencing tumor samples and artificial intelligence (AI) allow fast systematic prediction neoantigens. This study investigates feasibility, safety, immunity, anti-tumor potential the peptide-based neoantigen vaccine, EVX-01, including novel CD8+ T-cell inducing...
Novel single-cell-based technologies hold the promise of matching T cell receptor (TCR) sequences with their cognate peptide-MHC recognition motif in a high-throughput manner. Parallel capture TCR transcripts and is enabled through use reagents labeled DNA barcodes. However, analysis annotation such single-cell sequencing (SCseq) data are challenged by dropout, random noise, other technical artifacts that must be carefully handled downstream processing steps. We here propose rational,...
Background Neoantigens can serve as targets for T cell-mediated antitumor immunity via personalized neopeptide vaccines. Interim data from our clinical study NCT03715985 showed that the peptide-based neoantigen vaccine EVX-01, formulated in liposomal adjuvant, CAF09b, was safe and able to elicit EVX-01-specific cell responses patients with metastatic melanoma. Here, we present results dose-escalation part of study, evaluating feasibility, safety, efficacy, immunogenicity EVX-01 addition...
CD8+ T-cells are essential for controlling and resolving SARS-CoV-2 infection, yet their antigen-specific resolution in relation to disease severity, functional dynamics during acute long-term memory formation remain incompletely understood. Using comprehensive longitudinal profiling of 553 immunogenic antigens across globally prevalent HLAs, we identified T-cell responses that were either critical early viral clearance or associated with severe outcomes. During patients COVID-19 exhibited a...
The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal mRNA and protein expression among populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on at single-cell level, present study maps throughout MCT MCTC in healthy skin, gut lung tissues. cells had both higher frequency of protein-containing protein-staining intensity than population. Among tissues, skin MCs...
As the patents of originator biologics are expiring, biosimilar versions becoming available for treatment inflammatory bowel disease (IBD). However, published switch studies first infliximab biosimilar, CT-P13, have delivered ambiguous results that could be interpreted as showing a trend towards inferior effectiveness in Crohn's (CD) compared with ulcerative colitis (UC). The aim this study was to investigate and safety switching IBD patients from Remicade CT-P13.
Adoptive cell therapy (ACT) has shown promising results for the treatment of cancer and viral infections. Successful ACT relies on ex vivo expansion large numbers desired T-cells with strong cytotoxic capacity in persistence, which constitutes greatest challenge to current strategies. Here, this study, we present a novel technology antigen-specific T-cells; artificial antigen-presenting scaffolds (Ag-scaffolds) consisting dextran-polysaccharide backbone, decorated combinations peptide-Major...
Alkaline sphingomyelinase (NPP7) is expressed by intestinal epithelial cells and crucial for the digestion of dietary sphingomyelin. NPP7 also inactivates proinflammatory mediators including platelet-activating factor lysophosphatidylcholine. The aim this study was to examine a potential role in homeostasis immune system. We quantified numbers B-lymphocytes, plasma cells, T-lymphocytes regulatory (Tregs), natural killer dendritic macrophages, neutrophils, small large intestines, mesenteric...
T lymphocytes exert important homeostatic functions in the healthy intestinal mucosa, whereas case of colorectal cancer (CRC), infiltration into tumor is crucial for an effective anti-tumor immune response. In both situations, recruitment mechanisms tissues are essential immunological deciding outcome. The largely dependent on their expression various chemokine receptors. aim this study was to identify potential receptors involved normal human colonic mucosa and CRC tissue, respectively, by...
9551 Background: Despite the advances made with CPIs such as aPD-1/aPD-L1, aCTL4 and aLAG-3 there still is a significant unmet medical need for patients metastatic cancers. As it has been shown that effect of are mediated by tumor specific T cells, one strategy increasing treatment success to actively induce cells. Here, we report on first-in-human clinical trial evaluating personalized neoantigen vaccine (EVX-01) in melanoma. Methods: Stage-IV melanoma were treated standard aPD-1...
Abstract Novel single-cell based technologies hold the promise of matching T cell receptor (TCR) sequences with their cognate peptide-MHC recognition motif in a high-throughput manner. Parallel capture TCR transcripts and is enabled through use reagents labeled DNA barcodes. However, analysis annotation such sequencing (SCseq) data challenged by dropout, random noise, other technical artifacts that must be carefully handled downstream processing steps. We here propose rational, data-driven...