A5100341197- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- T-cell and Retrovirus Studies
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- HIV Research and Treatment
- Fungal Infections and Studies
- Insect and Pesticide Research
- Antifungal resistance and susceptibility
- Plant Virus Research Studies
- interferon and immune responses
- Influenza Virus Research Studies
- Macrophage Migration Inhibitory Factor
- Biosensors and Analytical Detection
- Antimicrobial Resistance in Staphylococcus
- HIV, Drug Use, Sexual Risk
- Animal Disease Management and Epidemiology
- COVID-19 epidemiological studies
- PI3K/AKT/mTOR signaling in cancer
- Computational Drug Discovery Methods
Lanzhou Veterinary Research Institute
2022-2024
Chinese Academy of Agricultural Sciences
2011-2024
Shihezi University
2022-2024
Harbin Veterinary Research Institute
2011-2020
Yonsei University
2020
University of New Mexico
2020
Fudan University Shanghai Cancer Center
2020
Zhejiang University
2020
Chinese PLA General Hospital
2020
Peking University
2014-2020
Membrane proteins within the sieve element-companion cell complex have essential roles in physiological functioning of phloem. The monoclonal antibody line RS6, selected from hybridomas raised against elements isolated California shield leaf (Streptanthus tortuosus; Brassicaceae) tissue cultures, recognizes an antigen Arabidopsis (Arabidopsis thaliana) ecotype Columbia that is associated specifically with plasma membrane elements, but not companion cells, and accumulates at earliest stages...
ABSTRACT In the past 5 years, an atypical clinical outbreak of avian leukosis virus subgroup J (ALV-J), which contains a unique 205-nucleotide deletion in its 3′ untranslated region (3′UTR), has become epidemic chickens China. To determine role pathogenicity ALV-J, pair viruses were constructed and rescued. The first was ALV-J Chinese isolate (designated HLJ09SH01) containing 3′UTR. second chimeric clone 3′UTR sequence corresponding to prototype virus. replication rescued (rHLJ09SH01...
The Crimean Congo Hemorrhagic Fever Virus (CCHFV) is a tick-borne bunyavirus of the Narovirus genus, which causative agent (CCHF). CCHF endemic in Africa, Middle East, Eastern Europe and Asia, with high case-fatality rate up to 50% humans. Currently, there are no approved vaccines or effective therapies available for CCHF. GEM-PA safe, versatile carrier system, offers cost-efficient, high-throughput platform recovery purification subunit proteins vaccines. In present study, based on surface...
Bats are reservoirs for diverse coronaviruses, including swine acute diarrhea syndrome coronavirus (SADS-CoV). SADS-CoV has been reported to have broad cell tropism and inherent potential cross host species barriers dissemination. We rescued synthetic wild-type using one-step assembly of a viral cDNA clone by homologous recombination in yeast. Furthermore, we characterized replication vitro neonatal mice. found that caused severe watery diarrhea, weight loss, 100% fatality rate 7- 14-day-old...
Infectious bursal disease (IBD) is one of the main threats to poultry industry worldwide. In China, very virulent IBD virus (vvIBDV) prevalent strain, causing inflammation, immunosuppression, and high mortality in young chickens. To determine whether this acute inflammation can trigger lesions or even death chickens, it important study mechanism vvIBDV pathogenicity. Thus, current study, we investigated response, lesions, chickens caused by at different time points post infection. Results...
Accumulating evidence suggests that p53 is involved in viral infection. However, it remains elusive whether avian orchestrates leukosis virus (ALV) replication. We showed recruits the histone deacetylase 1 and 2 (HDAC1/2) complex to ALV promoter shut off ALV's activity HDAC1/2 binding was abolished absence of p53. Moreover, we collected samples ALV-infected chickens found acetylation status ALV-bound H3 H4 histones correlated with viremia. HDAC inhibitors (HDACi) potently increase...
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered emerging alphacoronavirus. SADS-CoV shares over 90% genome sequence identity with bat alphacoronavirus HKU2. was associated severe and high mortality rates in piglets. Accurate serological diagnosis of infection key managing the SADS-CoV. However, thus far there have been no effective antibody-based diagnostic tests for diagnose exposure. Here, monoclonal antibody (MAb) 6E8 against N protein accurately recognized...
Introduction The Crimean-Congo hemorrhagic fever virus (CCHFV), the most geographically widespread tick-borne virus, is endemic in Africa, Eastern Europe and Asia, with infection resulting mortality up to 30% of cases. Currently, there are no approved vaccines or effective therapies available for CCHF. CCHFV should only be manipulated BSL-4 laboratory, which has severely hampered basic seroprevalence studies. Methods In present study, two antibody detection methods forms an enzyme-linked...
Subgroup J avian leukosis virus (ALV-J) was first isolated from meat-type chickens that had developed myeloid and since 2008, ALV-J infections in have become widespread China. A comparison of the sequence epidemic isolates with HPRS-103, prototype virus, revealed several distinct features, one which is a 19-nucleotide (nt) insertion leader sequence. To determine role 19-nt pathogenicity, pair viruses were constructed rescued. The an Chinese isolate (designated rSD1009) containing its second...
Diarrhea is one of the most important problems associated with production piglets, which have a wide range possible pathogens. This study identified strain porcine sapelovirus (PSV) by using next-generation sequencing (NGS) technologies as pathogen among fecal samples in pig herd. Phylogenetic analysis showed that PSV isolates shared unique polyprotein and clustered Chinese before 2013. The was then isolated named GS01. vitro vivo biological characteristics this virus were described. Our...
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered bat-origin with fatal pathogenicity for neonatal piglets. There no vaccine to prevent SADS-CoV infection or clinically approved drugs targeting SADS-CoV. Therefore, unraveling cellular factors that regulate cell entry critical understanding the viral transmission mechanism and provides potential therapeutic target cure. Here, we showed Type I interferon (IFN-I) pretreatment potently blocks into cells using lentiviral...
Background: The SARS-CoV-2 epidemic is currently spreading across the world. A better knowledge of viral loads at different stages disease progression would help inform clinical management and prevent nosocomial transmission. Methods: More than 3,000 respiratory, stool, serum urine samples were collected from 96 laboratory-confirmed COVID-19 patients. RNA load was measured, relationship between data severity analyzed. Findings: All patients confirmed by testing respiratory specimens, 54.22%...
Abstract Drug repurposing may be a pivotal means of fulfilling urgent needs for treatment the novel coronavirus disease 2019 (COVID-19), but current studies on drug COVID-19 seem to show lack consensus in their candidate focus. Using bibliometric methods non-expert perspective, review 34 published articles and drug-repurposing, we investigated obvious less points candidates. To establish these two types consensus, first implemented document clustering. Within set five clustered papers,...
Bats are reservoirs for diverse coronaviruses, including swine acute diarrhea syndrome coronavirus (SADS-CoV). SADS-CoV was first identified in diarrheal piglets 2017. As a novel alphacoronavirus, shares ~95% identity with bat alphacoronavirus HKU2. has been reported to have broad cell tropism and inherent potential cross host species barriers dissemination. Thus far, no effective antiviral drugs or vaccines available treat infections SADS-CoV. Therefore, knowledge of the protein-coding gene...