A5071675930- Ophthalmology and Visual Impairment Studies
- Corneal surgery and disorders
- Connexins and lens biology
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Adenosine and Purinergic Signaling
- Microbial Metabolic Engineering and Bioproduction
- Metal-Catalyzed Oxygenation Mechanisms
- Renal and related cancers
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Neurological Disease Mechanisms and Treatments
- Corneal Surgery and Treatments
- Retinopathy of Prematurity Studies
- Retinal Diseases and Treatments
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Spaceflight effects on biology
- Liver physiology and pathology
- Intraocular Surgery and Lenses
- Enzyme Catalysis and Immobilization
- Advanced Glycation End Products research
- Wound Healing and Treatments
- Telomeres, Telomerase, and Senescence
- Cancer, Stress, Anesthesia, and Immune Response
Wenzhou Medical University
2012-2024
Zhejiang Lab
2024
Affiliated Eye Hospital of Wenzhou Medical College
2011-2023
Jiangnan University
2019-2023
South China University of Technology
2019-2023
Guangzhou Medical University
2023
Southern Medical University
2020
Central South University
2016
Ministry of Health of the Russian Federation
2010
Daping Hospital
2010
MicroRNAs (miRNAs) are endogenous short chain (∼ 22-nucleotide) noncoding RNAs that inhibit protein translation through binding to target mRNAs. Recent studies have implied miRNAs play a regulatory role in corneal development. Here we profile their involvement epithelial renewal, develop an miRNA-target network affects wound healing outcome, and investigate the function of miR-204 this response.NanoString nCounter technology bioinformatics analyzed miRNA expression levels targets during...
l-Isoleucine dioxygenase (IDO) directly catalyzes the C–H bond hydroxylation of several hydrophobic aliphatic amino acids. However, ambiguous selectivity IDO prevents its application in chiral hydroxy acid production. The l-norleucine by IDO, which produces 4-hydroxynorleucine and 5-hydroxynorleucine with obvious regioselectivity, was used to investigate mechanism regioselectivity. Along computational structural analysis high-throughput screening, structure revealed single-site variants...
As the most serious of many worse new pathological changes caused by diabetes, there are risk factors for occurrence and development diabetic retinopathy (DR). They mainly include hyperglycemia, hypertension, hyperlipidemia so on. Among them, hyperglycemia is critical cause, plays a vital role in DR. High-sucrose diets (HSDs) lead to elevated blood glucose levels vivo, which, through oxidative stress, inflammation, production advanced glycation end products (AGEs) vascular endothelial growth...
The development of myopia is associated with decreased ocular scleral collagen synthesis in humans and animal models. Collagen is, part, under the influence cyclic adenosine monophosphate (cAMP). We investigated associations between cAMP, guinea pigs, by human fibroblasts (HSFs). Form-deprived (FDM) was induced unilateral masking pig eyes. Scleral cAMP levels increased selectively FDM eyes returned to normal after unmasking recovery. Unilateral subconjunctival treatment adenylyl cyclase (AC)...
This study used dopamine D2 receptor (D2R) knockout (KO) mice to investigate the role of D2R activity in development form-deprivation myopia (FDM). Sulpiride, a antagonist, was administered systemically into wild-type (WT) validate involvement FDM development.The KO and WT C57BL/6 were subjected FDM. Wild-type received daily intraperitoneal injections sulpiride, 8 μg/g body weight, for period 4 weeks. The refraction, corneal radius curvature, ocular axial components measured at week...
Purpose.: To critically evaluate whether the adenosine A2A receptor (A2AR) plays a role in postnatal refractive development mice. Methods.: Custom-built biometric systems specifically designed for mice were used to assess of relative myopia by examining refraction and biometrics A2AR knockout (KO) wild-type (WT) littermates between days (P)28 P56. Ocular dimensions measured customized optical coherence tomography (OCT), state eccentric infrared photorefraction (EIR), corneal radius curvature...
Purpose: The purpose of this study was to evaluate the role canonical Wnt signaling in development myopia. Methods: Plasma from adult patients with myopia, myopic animal models including adenomatous polyposis coli (APC) gene mutation mouse model, and form deprivation (FD) induced model myopia were used. Niclosamide, a pathway inhibitor, orally administrated models. levels DKK-1 determined by using enzyme-linked immunosorbent assay. Refraction, vitreous chamber depth (VCD), axial length (AL),...
As the most serious of many worse new pathological changes caused by diabetes, there are risk factors for occurrence and development DR. It mainly includes hyperglycemia, hypertension, hyperlipidemia so on. Among them, hyperglycemia is critical cause, which plays a key role in DR.High-sucrose diets(HSDs) lead to elevated blood glucose levels vivo, which, through oxidative stress, inflammation, production AGEs VEGF, causes plenty damages retina ultimately bring about loss vision. The existing...
Uveal melanoma is the most common primary intraocular tumor in adults western countries. It associated with very severe visual morbidity and may lead to distant metastases even after successful treatment of tumor. In order gain better insight into molecular mechanisms related tumorigenesis metastasis uveal melanoma, we used next-generation sequencing technology (SOLiD, Life Technologies) acquire global transcriptome alteration between posterior cells normal melanocyte.From mRNAs cultured...
Ambient lighting is essential for ocular development in many species, however, disruption diurnal cycle can affect the refraction and axial growth of eye. This study investigated effects prolonged daily on various optical components eye by raising C57BL/6 mice under three different light/dark cycles (18/6, 12/12 6/18). Egr-1 mRNA expression, apoptosis histology retina size scleral fibrils were evaluated these cycles. Results showed that there was a trend myopic development, increasing...
Adenosine A2A receptors (A(2A)Rs) in bone marrow-derived cells (BMDCs) are involved regulation of inflammation and outcome several CNS injuries; however their relative contribution to traumatic brain injury (TBI) is unknown. In this study, we created a mouse cortical impact model, BMDC A(2A)Rs were selectively inactivated wild-type (WT) mice or reconstituted global A(2A)R knockout (KO) (i.e. inactivation non-BMDC A(2A)Rs) by marrow transplantation. When compared with WT mice, selective...
Purpose: We used a mouse model to explore the role of endoplasmic reticulum membrane protein complex subunit 3 (EMC3) in mammalian retinal development. Methods: The transcription pattern Emc3 C57BL/6 mice was analyzed by situ hybridization. To effects EMC3 absence on development, Cre-loxP system generate retina-specific knockout (Emc3flox/flox, Six3-cre+; CKO). Morphological changes retina E13.5, E17.5, P0.5, and P7 were observed via hematoxylin eosin staining. Immunofluorescence staining...
Hydroxyl amino acids have tremendous potential applications in food and pharmaceutical industries. However, available dioxygenases are limited for selective efficient hydroxylation of free acids. Here, we identified a 2-oxoglutarate-dependent dioxygenase from Kutzneria albida by gene mining characterized the encoded protein (KaPH1). KaPH1 was estimated to molecular weight 29 kDa. The optimal pH temperature its l-proline activity were 6.5 30 °C, respectively. Km kcat values 1.07 mM 0.54 s-1,...
Inadequate remnant volume and regenerative ability of the liver pose life-threatening risks to patients after partial transplantation (PLT) or hepatectomy (PHx), while few clinical treatments focus on safely accelerating regeneration. Recently, we discovered that supplementing 5-aminolevulinate (5-ALA) improves cold adaptation functional recovery, leading us uncover a correlation between 5-ALA metabolic activities post-PLT recovery. In mouse 2/3 PHx model, supplements enhanced regeneration,...
Myopia incidence in China is rapidly becoming a very serious sight compromising problem large segment of the general population. Therefore, delineating underlying mechanisms leading to myopia will markedly lessen likelihood other complications. In this regard, there some evidence that patients afflicted with familial adenomatous polyposis (FAP), havean coli (APC) mutation and higher myopia. To clarify possible association, we determined whether changes pertinent biometric biochemical...
The transparency of the ocular lens is essential for refracting and focusing light onto retina, controlled by many factors signaling pathways. Here we showed a critical role chromatin remodeler zinc finger HIT-type containing 1 (Znhit1) in maintaining transparency.To explore roles Znhit1 development, cre-loxp system was used to generate lens-specific knockout mice (Znhit1Mlr10-Cre; cKO). Morphological changes lenses were examined using hematoxylin eosin staining. RNA sequencing (RNA-seq)...
The aim of study was to establish Rdh12-associated inherited retinal disease (Rdh12-IRD) mouse model and identify the best timepoint for gene therapy.We induced degeneration in Rdh12-/- mice using a bright light. We clarified establishment Rdh12-IRD by analyzing thickness layers electroretinography (ERG). received subretinal injection adeno-associated virus 2/8-packaged Rdh12 cDNA treatment. evaluated visual function structure treated untreated eyes therapy.Rdh12-IRD showed significant...