A5005870843- Liver Disease Diagnosis and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects of Natural Compounds
- Peroxisome Proliferator-Activated Receptors
- Salivary Gland Disorders and Functions
- Adipose Tissue and Metabolism
- Phytochemicals and Medicinal Plants
- Glaucoma and retinal disorders
- Silymarin and Mushroom Poisoning
- Corneal surgery and disorders
- Ophthalmology and Visual Impairment Studies
- Diet, Metabolism, and Disease
- Biochemical Acid Research Studies
- Dietary Effects on Health
- Retinal Diseases and Treatments
- Studies on Chitinases and Chitosanases
- Polyamine Metabolism and Applications
- Liver physiology and pathology
- Ginseng Biological Effects and Applications
- Bioinformatics and Genomic Networks
- Amino Acid Enzymes and Metabolism
- HER2/EGFR in Cancer Research
- Genomics and Phylogenetic Studies
- Liver Diseases and Immunity
Center for Cancer Research
2016-2024
China Pharmaceutical University
2012-2024
National Cancer Institute
2016-2024
National Institutes of Health
2016-2024
Kunming University of Science and Technology
2024
Sir Run Run Shaw Hospital
2023
Zhejiang University
2023
Inner Mongolia Medical University
2021-2022
China Agricultural University
2022
State Key Laboratory of Natural Medicine
2015-2021
Abstract Determining the structure and phenotypic context of molecules detected in untargeted metabolomics experiments remains challenging. Here we present reverse as a discovery strategy, whereby tandem mass spectrometry spectra acquired from newly synthesized compounds are searched for public datasets to uncover associations. To demonstrate concept, broadly explored multiple classes metabolites humans, including N -acyl amides, fatty acid esters hydroxy acids, bile conjugated acids. Using...
Background and Aims Peroxisome proliferator‐activated receptor α (PPARα) regulates fatty acid transport catabolism in liver. However, the role of intestinal PPARα lipid homeostasis is largely unknown. Here, was examined for its modulation obesity NASH. Approach Results Intestinal activated acid‐binding protein 1 (FABP1) up‐regulated humans with high‐fat diet (HFD)–fed mice as revealed by using human intestine specimens or HFD/high‐fat, high‐cholesterol, high‐fructose (HFCFD)‐fed C57BL/6N...
Abstract Bacteria in the gastrointestinal tract produce amino acid bile amidates that can affect host-mediated metabolic processes 1–6 ; however, bacterial gene(s) responsible for their production remain unknown. Herein, we report salt hydrolase (BSH) possesses dual functions metabolism. Specifically, identified a previously unknown role BSH as an amine N -acyltransferase conjugates amines to acids, thus forming (BBAAs). To characterize this activity, used pharmacological inhibition of BSH,...
Here, we present PatMatch, an efficient, web-based pattern-matching program that enables searches for short nucleotide or peptide sequences such as cis -elements in small domains and motifs protein sequences. The can be used to find matches a user-specified sequence pattern described using ambiguous codes powerful flexible syntax based on regular expressions. A recent upgrade has improved performance now supports both mismatches wildcards single pattern. This enhancement been achieved by...
Nonalcoholic steatohepatitis (NASH) is the progressive stage of nonalcoholic fatty liver disease that may ultimately lead to cirrhosis and cancer, there are few therapeutic options for its treatment. Glycyrrhizin (GL), extracted from traditional Chinese medicine liquorice, has potent hepatoprotective effects in both preclinical animal models humans. However, little currently known about mechanisms treating NASH. To explore GL on NASH, or active metabolite glycyrrhetinic acid (GA) was...
PPARα (PPARA), expressed in most oxidative tissues, is a major regulator of lipid homeostasis; hepatic PPARA plays critical role during the adaptive fasting response by promoting FA oxidation (FAO). To clarify whether extrahepatic activity can protect against overload when impaired, accumulation was compared WT (Ppara+/+), total body Ppara-null (Ppara-/-), and hepatocyte-specific (PparaΔHep) mice that were fasted for 24 h. Histologic staining indicated reduced PparaΔHep versus Ppara-/- mice,...
Liver resection is the first-line treatment for primary liver cancers, providing potential a cure. However, concerns about post-hepatectomy failure (PHLF), leading cause of death following extended resection, have restricted population eligible patients. Here, we engineered clinical-grade bioartificial (BAL) device employing human-induced hepatocytes (hiHeps) manufactured under GMP conditions. In porcine PHLF model, hiHep-BAL showed remarkable survival benefit. On top supportive function,...
Metabolic dysfunction-associated steatohepatitis (MASH) - previously described as nonalcoholic (NASH) is a major driver of liver fibrosis in humans, while key determinant all-cause mortality disease independent MASH occurrence. CCAAT/enhancer binding protein α (CEBPA), versatile ligand-independent transcriptional factor, has an important function myeloid cells, and under clinical evaluation for cancer therapy. CEBPA also expressed hepatocytes regulates glucolipid homeostasis; however, the...
Summary: We describe multiple methods for accessing and querying the complex integrated cellular data in BioCyc family of databases: access through file formats, Application Program Interfaces (APIs) LISP, Perl Java, SQL BioWarehouse relational database.
Acetaminophen (APAP) overdose is the leading cause of drug-induced acute liver failure in Western countries. Glycyrrhizin (GL), a potent hepatoprotective constituent extracted from traditional Chinese medicine liquorice, has potential clinical use treating APAP-induced failure. The present study determined effects and underlying mechanisms action GL its active metabolite glycyrrhetinic acid (GA). Various administration routes pharmacokinetics–pharmacodynamics analyses were used to...
Pregnane X receptor (PXR) activation exhibits anti-inflammatory effects via repressing nuclear factor-κB (NF-<i>κ</i>B); however, its overactivation may disrupt homeostasis of various enzymes and transporters. Here we found that ginsenosides restore PXR/NF-<i>κ</i>B signaling in inflamed conditions without disrupting PXR function normal conditions. The mechanisms regulating signals were determined both vitro vivo. Ginsenosides significantly inhibited NF-<i>κ</i>B restored the expression...
To compare the effects of daily injection versus continuous infusion a nonspecific dopamine agonist, apomorphine (APO), on refraction and ocular growth in normal postnatal mice with form-deprivation myopia (FDM).The C57BL/6 were subjected (or not) to monocular FD by covering left eye frosted goggle leaving right (fellow) uncovered. During days 28 56, both groups received APO (5 mg/kg/d) or vehicle either as intraperitoneal subcutaneous mini-pumps. After these treatments, binocular...
Although the functions of metabolic enzymes and nuclear receptors in controlling physiological homeostasis have been established, their crosstalk modulating disease has not explored. Genetic ablation xenobiotic-metabolizing cytochrome P450 enzyme CYP2E1 mice markedly induced adipose browning increased energy expenditure to improve obesity. deficiency activated expression hepatic peroxisome proliferator-activated receptor alpha (PPARα) target genes, including fibroblast growth factor (FGF)...
This study used dopamine D2 receptor (D2R) knockout (KO) mice to investigate the role of D2R activity in development form-deprivation myopia (FDM). Sulpiride, a antagonist, was administered systemically into wild-type (WT) validate involvement FDM development.The KO and WT C57BL/6 were subjected FDM. Wild-type received daily intraperitoneal injections sulpiride, 8 μg/g body weight, for period 4 weeks. The refraction, corneal radius curvature, ocular axial components measured at week...
Hepatocellular carcinoma (HCC) is a leading cause of cancer deaths worldwide, and an association between altered bile acid (BA) metabolism, down‐regulation farnesoid X receptor (FXR), which master regulator BA hepatocarcinogenesis has been documented. While global FXR deficiency in mice results spontaneous HCC with aging, the contribution tissue‐specific to remains unclear. In this study, prevalence hepatic tumors, expression genes related tumorigenesis, serum/liver levels were compared...
BackgroundHepatocyte is particularly vulnerable to apoptosis, a hallmark of many liver diseases. Although pro-apoptotic mechanisms have been extensively explored, less known about the hepatocyte-specific anti-apoptotic molecular events and it lacks effective approach combat hepatocyte apoptosis. We investigated effect mechanism farnesoid X receptor (FXR), strategies how target FXR for inhibiting apoptosis implicated in fibrosis.MethodsSensitivity was compared between wild type Fxr−/− mice...
Abstract Fulminant hepatitis (FH) is an incurable clinical syndrome where novel therapeutics are warranted. Withaferin A (WA), isolated from herb Withania Somnifera , a hepatoprotective agent. Whether and how WA improves D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced FH unknown. This study was to evaluate the role mechanism of in GalN/LPS-induced FH. To determine preventive therapeutic effects WA, wild-type mice were dosed with 0.5 h before or 2 after GalN treatment, followed by LPS...
Abstract The idiosyncratic characteristics and severity of acetaminophen (APAP) overdose-induced hepatotoxicity render identifying the predisposing factors mechanisms APAP-induced liver toxicity necessary urgent. Farnesoid X receptor (FXR) controls bile acid homeostasis modulates progression various diseases. Although global FXR deficiency in mice enhances APAP intoxication, mechanism remains elusive. In this study, an increased sensitivity to was found Fxr-null (Fxr−/−) mice, but not...
β-Lapachone (β-Lap) is anquinone oxidoreductase 1 (NQO1) target antitumor drug candidate in phase II clinical trials. The present study aimed to uncover the metabolic profile, enzyme kinetics, and isoforms for metabolism of β-Lap human liver intestine vitro. NQO1-mediated quinone reduction subsequent glucuronidation predominant pathway humans; a pair regioisomers (M1 M2) reduced glucuronides were major metabolites found from S9 incubations. overall clearance was 4754.90 μL/min/mg protein...