Abstract Biorefineries are a model for greener production processes, based on the concept of bioeconomy. Instead targeting first-generation biofuels—that compete with food supply—the focus relies lignocellulosic material, considering many aspects, such as sustainable fuel production, well valorization waste, an alternative to traditional petrochemical approach goods production. Especially, in tropical countries agricultural activities lead tremendous amounts biomass, resulting waste that has...

An inline method to investigate microbubble enhanced mass transfer and its effect on a model biocatalyst utilizing CO₂ for carboxylation.

The integrated process setup of reaction and separation in reactive distillation can be favorable for enzyme-catalyzed reactions that are often equilibrium-limited and/or inhibited by the product reaction. However, until now, no appropriate way has described implementation enzymes into columns. In this work, a special type biocatalytic coating commercially available structured packings was developed to enable enzymatic Lipase CALB immobilized within silica-gel based showed high long-term...

Abstract The application of enzymes presents a great advantage regarding highly selective reactions; however, it involves also challenges due to their sensitivity. Immobilization offers one strategy overcome those enabling enzyme stabilization, as well retention. In the present study, covalent attachment on hydrophilic amino‐functionalized carriers is found be most promising immobilization method for investigated reaction system. To achieve this, novel preparation silica particles with...

Abstract Tropical crops are an important source of wealth in many countries. The current agribusiness model is based on the production a final commodity, leading to organic by-products (biowastes) that cases contain bioactive compounds with potential added value. exploitation these foundation circular economy leads generation greener bioprocesses for industry foreseeable economic improvements systems. This review aims point out idle opportunities agricultural systems and their associated...

Flavonoids are polyphenolic secondary plant metabolites which possess antioxidant and anti-inflammatory properties. Besides, they have been shown to exhibit increased properties in their polymerized form. Catechins one of the attractive class flavonoids belong group flavan-3-ols. Polymerization catechins investigated numerous studies indicating requirement certain amount organic solvent provide solubility monomer. However, many research projects conducted recently replace toxic contaminants...

The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, process optimization this still a bottleneck that cannot be overcome with the established methods macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient aeration can or improved. Investigating transport model protein results kL values related to applied airstream comparison Comparing aerators at identical 160 and 60 1/h, resulting 25 44...

A circular phosphorus (P) bioeconomy is not only worthwhile for conserving limited mineral P reservoirs, but also minimizing negative environmental impacts caused by human-made alterations. Although an essential nutrient, most of the in concentrates based on cereals, legumes and oilseed byproducts organically bound to phytate. The latter cannot be efficiently utilized monogastric animals therefore diluted into environment through manure pathway. This review examines various strategies...

The Deepwater Horizon incident in the Gulf of Mexico 2010 released an unprecedented amount petroleum hydrocarbons 1500 meters below sea surface. Few studies have considered influence hydrostatic pressure on bacterial community development and activity during such spills. goal this study was to investigate response indigenous sediment microbial communities combination increased pressure, dispersant. Deep-sea samples collected from northern were incubated at atmospheric (0.1 MPa) elevated (10...

Abstract Unspecific peroxygenases have recently gained significant interest due to their ability catalyse the hydroxylation of non‐activated C−H bonds using only hydrogen peroxide as a co‐substrate. However, development preparative processes has so far mostly concentrated on benzylic hydroxylations liquid substrates. Herein, we demonstrate application peroxygenase for inert, gaseous substrate butane 2‐butanol in bubble column reactor. The influence feed rate and enzyme loading product...

Cascade reactions are the basis of life in nature and adapted to research industry an increasing manner. The focus this study is production high-value aromatic ester cinnamyl cinnamate, which can be applied flavors fragrances. A three-enzyme cascade was established realize synthesis, starting from corresponding aldehyde with situ cofactor regeneration a two-phase system. After characterization enzymes, screening different organic solvents carried out, whereby xylene found most suitable...

Abstract Biotechnological application of multiple enzymes in different phases for target compounds synthesis poses a significant challenge industrial process development. At the same time, growing demand natural flavors and fragrances opens up possibilities novel biotechnological processes to replace current chemical routes, with additional advantages such as avoiding harsh reaction conditions toxic chemicals, less by‐products system. Within complex processes, key unfolding their potential...

The utilization of oxidases suffers from insufficient oxygen availability due to its low solubility in gas-liquid multiphase systems. To overcome this limitation, is continuously supplied the reaction medium during course reaction, however, it causes significant gas consumption. In study, a novel experimental set-up was established by installing sintered frit porous sparger SpinChem® rotating bed reactor. Covalently immobilized glucose oxidase on epoxy-functionalized carriers used reactor...

Abstract Side streams from the milling industry offer excellent nutritional properties for animal feed; yet their use is constrained by elevated phosphorus (P) content, mainly in form of phytate. Biotechnological P recovery fosters sustainable management, transforming these into P-depleted feed through enzymatic hydrolysis. The mobilization not only enables by-products but also supports valorization feeds. Our study presents scalability and applicability process characterizes resulting rye...

Abstract When facing today’s scarcity of mineral phosphorus (P) resources and the environmental issues following enhanced P losses especially from agriculture, new solutions need to be implemented. In this framework, potential for a mechanical separation rich grain fraction wheat, rye, barley oats is investigated in order provide animal feed with reduced organic content. Thus, accumulation manure soils should prevented. Also, subsequent utilization separated P, which occurs form inositol...

Abstract The occurrence of organically bound phosphorus (P) as phytate in plant-based feeding material is a challenge for livestock farming due to limited utilization during the digestion by animal. Its excretion into environment through manure pathway, poses challenge, increased eutrophication and restrictions P. Hence, while routine supplementation phytase enzymes monogastric diets common practice, metabolically triggering endogenous plant wet-treatment prior can also lead better P...

Immobilisation plays an important role in the industrial application of enzymes. The stabilisation and reusability immobilised enzymes reduce cost catalyst facilitate their use continuously operated reactors. For this purpose, applicable type immobilisation needs to be identified. In study, we investigate conversion CDP PolyP CTP by NDP polyphosphate phosphotransferase 3 from Ruegeria pomeroyi (RpPPK2-3) describe covalent RpPPK2-3. order select a suitable carrier for RpPPK2-3, screening with...

One of the key elements in stirred tank reactor set-ups is submersed aeration system. Aeration with microbubbles provides high gas utility coupled prolonged enzyme stability by lowering interfacial area renewing rate depending on mode.

Abstract Carbon capture technology can be set up in combination with biocatalysis to utilize the bound CO 2 as substrate Kolbe‐Schmitt like enzymatic reaction. The exemplary whole cell biotransformation of catechol 2,3‐dihydroxybenzoic acid a triethanolamine‐mediated multiphase system shows increased equilibrium conversion. Apart from beneficial thermodynamics, inherent fluid properties triethanolamine is enabling easy application fine bubbles highly efficient gassing method minimize demand...

The importance of a compound that helps fight against influenza is, in times pandemic, self-evident. In order to produce these compounds vast quantities, many researchers consider continuous flow reactors chemical industry as next stepping stone for large scale production. For reasons, the synthesis N-acetylneuraminic acid (Neu5Ac) fixed-bed reactor by an immobilized epimerase and aldolase was investigated detail. enzymes showed high stability, with half-life > 173 days under storage...

Natural deep eutectic solvents (NADESs) were investigated for the activity and stability of laccase enzyme. Laccase was found to be active only in a low amount choline chloride containing NADESs while it maintained its both high amounts betaine NADESs. Rutin, as flavonoid monomer shows antioxidant, antibacterial, antiviral anti-inflammatory properties these are enhanced polymerised form. In this study, organic co-solvent that is conventionally used oligomerization rutin replaced with two...