A5029624802- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Eosinophilic Disorders and Syndromes
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Kruppel-like factors research
- Platelet Disorders and Treatments
- Blood groups and transfusion
- Transplantation: Methods and Outcomes
- Angiogenesis and VEGF in Cancer
- Virus-based gene therapy research
- Multiple Myeloma Research and Treatments
- CAR-T cell therapy research
- Acute Lymphoblastic Leukemia research
- Neutropenia and Cancer Infections
- Blood transfusion and management
- 3D Printing in Biomedical Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Peripheral Artery Disease Management
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cell Adhesion Molecules Research
- Organ Transplantation Techniques and Outcomes
- Photodynamic Therapy Research Studies
- Pregnancy and preeclampsia studies
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2024
Policlinico San Matteo Fondazione
2014-2024
University of Pavia
1992-2017
National Blood Transfusion Service
2016
Foundation Center
2007
Illinois College
2005
University of Illinois Urbana-Champaign
2005
Organ Transplantation and Immunology Institute
2004-2005
Policlinico S.Orsola-Malpighi
2001
University of Bologna
2001
Megakaryocytes associate with the bone marrow vasculature where they convert their cytoplasm into proplatelets that protrude through vascular endothelium lumen and release platelets. The extracellular matrix (ECM) microenvironment plays a critical role in regulating these processes. In this work we demonstrate that, among ECM components, fibronectin, type IV collagen, laminin are most abundant around sinusoids constitute pericellular surrounding megakaryocytes. Most importantly, report, for...
Abstract The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. goal the present study was to elucidate whether hAMCs can exert beneficial effects on infarcted rat hearts, in particular through cardioprotection and angiogenesis. Moreover, we aimed identify putative active mediators. were isolated, expanded, characterized. In vitro, conditioned medium from hAMC (hAMC-CM) exhibited cytoprotective proangiogenic properties....
A phase II dose-escalation trial was conducted to ascertain low-dose thalidomide safety and response in patients with advanced myelofibrosis myeloid metaplasia (MMM).Thalidomide administered together current therapy 63 patients, starting at 50 mg daily increasing 400 as tolerated.Half of the sustained doses more than 100 drop-out rate 51% 6 months: lower high baseline fatigue score. At efficacy analysis, anemia ameliorated 22% transfusions were eliminated 39% transfusion-dependent patients....
BACKGROUND: Extracorporeal photochemotherapy (EPC) has recently been proposed for the treatment of adults with either acute or chronic GVHD. However, data on children given this therapy are scarce. A Phase I‐II study was carried out EPC in experiencing GVHD after allogeneic transplantation HPCs. STUDY DESIGN AND METHODS: Nine patients steroid‐resistant, grade II‐IV and 14 GVHD, all whom had refractory to at least one line treatment, were enrolled analyzed. The median age 10.3 years (range,...
Extracorporeal photochemotherapy (ECP) is a valid therapeutic option in the treatment of acute and chronic graft-versus-host disease (aGVHD cGVHD, respectively). No standard clinical laboratory criteria response to ECP are available at moment.Clinical variables on 73 pediatric patients with aGVHD (n = 50) cGVHD 23) were correlated survival.An overall (OR) was obtained 34 50 (68%) 16 23 (69.5%) patients. Steroid tapering within 30 days 1.3 mg/kg OR (p 0.004) sole highly significant...
Purpose In the WHO diagnostic classification, prefibrotic myelofibrosis (pre-MF) is included in category of primary (PMF). However, strong evidence for this position lacking. Patients and Methods We investigated whether pre-MF may be aligned along a clinical biological continuum 683 consecutive patients who received diagnosis PMF. Results As compared with PMF-fibrotic type, (132 cases) showed female dominance, younger age, higher hemoglobin, platelet count, lower white blood cell smaller...
<b><i>Background:</i></b> Extracorporeal photochemotherapy (ECP) for chronic lung allograft dysfunction (CLAD) has been reported as beneficial in a few short-term studies. <b><i>Objectives:</i></b> In this retrospective cohort study on 48 CLAD patients treated by ECP (off-line technique) period of >8 years (compared to 58 controls), we explored potential predictors survival and response. <b><i>Methods:</i></b>...
Endothelial progenitor cells (EPCs) are present in circulation and contribute to vasculogenesis adults. We measured the number of circulating EPCs patients with myelofibrosis myeloid metaplasia (MMM), we examined relationship between severity MMM disease process.The was by assaying CD34+CD133+ vascular endothelial growth factor receptor 2 (VEGFR2) -positive cell phenotype 110 patients, 16 other Philadelphia-negative chronic myeloproliferative disorders (Ph-negative CMPDs), 14 healthy...
BACKGROUND: Chronic graft‐versus‐host disease (cGVHD) classification has recently been improved by the National Institutes of Health (NIH); patients’ stratification with those new criteria implications for patients' prognosis. STUDY DESIGN AND METHODS: To assess whether NIH consensus (NCC) better predicts survival and response to extracorporeal photochemotherapy (ECP), identify variables associated survival, we retrospectively analyzed 102 patients cGVHD reclassified according NCC treated...
Although many trials are currently investigating the safety and efficacy of convalescent plasma (CP) in critically ill COVID-19 patients, there is a paucity ongoing published studies evaluating CP donors' side. This retrospective study reports first Italian experience on selection donations.Patients aged 18-68 years who had recovered from at least 2 weeks previously were recruited between March 18 June 30, 2020 protocol hospitals Pavia Mantova.During period, 494 512 donors judged eligible...
Several studies have demonstrated that miRNA are involved in cardiac development, stem cell maintenance, and differentiation. In particular, it has been shown miRNA133, miRNA1, miRNA499 progenitor differentiation into cardiomyocytes. However, is unknown whether different may act synergistically to improve We used mouse P19 cells as a cardiogenic model. miRNA499, or miRNA133 were transiently over-expressed individually combinations. The over-expression of alone increased the number beating...
The current available techniques to wash out DMSO from thawed umbilical cord blood (UCB) units are based essentially on standard centrifugation in an open system with various degrees of cell loss.We evaluated the capacity a new automated closed device (Cytomate, Baxter, IL) UCB units, saving at same time progenitor and accessory cells terms CD34+ MNCs. We modified software calculated recovery 25 units. Moreover, we set up gas chromatographic method exactly detect removal rate.To evaluate...
Demonstrate the safety and effectiveness of highly purified CD133+ autologous stem cells in critical limb ischemia (CLI). Prospective single-center not randomized. Clinicaltrials.gov identifier: NCT01595776 Eight patients with a history stable CLI were enrolled period 2 years. After bone marrow stimulation single leukapheresis collection, immunomagnetic cell selection was performed. buffer phosphate suspension administered intramuscularly. Muscular arterial contrast enhanced ultra sound...
Increased mobilization of circulating endothelial progenitor cells may represent a new biological hallmark myeloproliferative neoplasms. We measured colony forming (ECFCs) in 106 patients with primary myelofibrosis, fibrotic stage, 49 prefibrotic 59 essential thrombocythemia or polycythemia vera, and 43 normal controls. Levels ECFC frequency for patient's characteristics were estimated by using logistic regression univariate multivariate setting. The sensitivity, specificity, likelihood...
Background Extracorporeal photochemotherapy ( ECP ) is an effective cell therapy employed in several diseases, including graft‐versus‐host disease GVHD and organ rejection after transplantation. When performed using the off‐line technique, mononuclear MNC collection by leukapheresis necessary for further manipulation. Semiautomated apheresis systems require experienced personnel to obtain a good collection; automated device, able efficiently collect s with high purity, desirable. We compared...