A5025881321- Renal Diseases and Glomerulopathies
- Mast cells and histamine
- Renal Transplantation Outcomes and Treatments
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Chronic Lymphocytic Leukemia Research
- Atherosclerosis and Cardiovascular Diseases
- Renal and related cancers
- Aquatic Ecosystems and Phytoplankton Dynamics
- Antifungal resistance and susceptibility
- Cynara cardunculus studies
- Marine Toxins and Detection Methods
- Metabolomics and Mass Spectrometry Studies
- Apelin-related biomedical research
- Complement system in diseases
- Vascular Tumors and Angiosarcomas
- Cardiac Imaging and Diagnostics
- Cardiovascular Function and Risk Factors
- Sarcoma Diagnosis and Treatment
- Paraoxonase enzyme and polymorphisms
- Cardiomyopathy and Myosin Studies
- Polyomavirus and related diseases
- Hormonal Regulation and Hypertension
- Urticaria and Related Conditions
- Pancreatic and Hepatic Oncology Research
- Drug Transport and Resistance Mechanisms
University of Toledo Medical Center
1986-2025
University of Toledo
2012-2024
University of Louisville
1983
Jewish Hospital
1983
As life expectancy continues to increase, clinicians are challenged by age-related renal impairment that involves podocyte senescence and glomerulosclerosis. There is now compelling evidence lithium has a potent antiaging activity ameliorates brain aging increases longevity in Drosophila Caenorhabditis elegans. the major molecular target of action multitasking protein kinase recently implicated variety diseases, glycogen synthase 3β (GSK3β) overexpressed hyperactive with age glomerular...
A disintegrin-like metalloproteinase with thrombospondin motifs–16 ( Adamts16 ) is an important candidate gene for hypertension. The goal of the present study was to further assess candidacy by targeted disruption this in a rat genetic model generated manipulating genome Dahl Salt–sensitive (S) using zinc-finger nucleases, wherein mutant had 17 bp deletion first exon , introducing stop codon transcript. Systolic blood pressure (BP) homozygous rats lower 36 mmHg compared BP S rats. exhibited...
Alport syndrome (AS) is a genetic disorder caused by mutations in COL4A3, COL4A4, and COL4A5 that affect the synthesis, assembly, deposition, or function of collagen IV α3α4α5 network glomerular basement membranes (GBMs). This causes pathology from microscopic hematuria to progressive renal disease. Although early treatment with angiotensin-converting enzyme inhibitors can delay progression, patients will eventually develop end-stage disease (ESRD).1 The risk timing ESRD are affected sex...
Objective- Dysregulated proliferation of vascular smooth muscle cells (VSMC) plays an essential role in neointimal hyperplasia. CD36 functions critically atherogenesis and thrombosis. We hypothesize that regulates VSMC contributes to the development obstructive diseases. Approach Results- found by immunofluorescent staining was highly expressed human vessels with Using guidewire-induced carotid artery injury shear stress-induced intima thickening models, we compared hyperplasia Apoe-/-,...
Microcystins are potent hepatotoxins that have become a global health concern in recent years. Their actions at-risk populations with pre-existing liver disease is unknown. We tested the hypothesis No Observed Adverse Effect Level (NOAEL) of Microcystin-LR (MC-LR) established healthy mice would cause exacerbation hepatic injury murine model (Leprdb/J) Non-alcoholic Fatty Liver Disease (NAFLD). Ten-week-old male Leprdb/J were gavaged 50 μg/kg, 100 μg/kg MC-LR or vehicle every 48 h for 4 weeks...
Pathogenesis of hepatocellular carcinoma (HCC), which kills millions annually, is poorly understood. Identification risk factors and modifiable determinants mechanistic understanding how they impact HCC are urgently needed.We sought early prognostic indicators in C57BL/6 mice, we found were prone to developing this disease when fed a fermentable fiber-enriched diet. Such markers used phenotype interrogate stages development. Their human relevance was tested using serum collected...
DNA binding and adduct formation of aflatoxin B 1 (AFB ) was studied in cultured bladder tracheobronchial explants from human, monkey, dog, hamster rat. Explants were exposed to [ 3 H]AFB (1 μM final concentration) PFMR-4 medium (pH 7.4) without serum for 24 h, after which epithetial cell isolated by hydroxylapatite chromatography. Binding (μmol AFB /mol deoxyribo-nucledetide, mean ± SD) higher tissues (human, 2.2 2.4; rat, 5.7 10.6 6.6; 134.6 44.6) than 1.5 2.3; 2.5 1.1; 3.8 5.2 hamster,...
Papraoxonase-1 (PON1) is a hydrolytic lactonase enzyme that synthesized in the liver and circulates attached to high-density lipoproteins (HDL). Clinical studies have demonstrated an association between diminished PON-1 progression of chronic kidney disease (CKD). However, whether decreased mechanistically linked renal injury unknown. We tested hypothesis absence inflammation CKD. Experiments were performed on control Dahl salt-sensitive rats (SSMcwi, hereafter designated SS rats) Pon1...
Journal Article The effects of mezlocillin, ticarcillin and placebo on blood coagulation bleeding time in normal volunteers Get access Pitambar Somani, Somani Search for other works by this author on: Oxford Academic PubMed Google Scholar Mary R. Smith, Smith Correspondence to: P. M.D., Division Clinical Pharmacology, Medical College Ohio, C.S. 10008, Toledo, OH, 43699, U.S.A. Amira Gohara, Gohara Kanti Kapila *Miles PharmaceuticalsWest Haven, CT, 06516, Antimicrobial Chemotherapy, Volume...
Paraoxonase-1 (PON-1) is a hydrolytic enzyme associated with HDL, contributing to its anti-inflammatory, antioxidant, and anti-atherogenic properties. Deficiencies in PON-1 activity result oxidative stress detrimental clinical outcomes the context of chronic kidney disease (CKD). However, it unclear if decrease mechanistically linked adverse cardiovascular events CKD. We investigated hypothesis that cardioprotective Dahl salt-sensitive model hypertensive renal disease. Experiments were...
Background: HCC is the most common primary liver cancer and a leading cause of cancer-related mortality. Gut microbiota large collection microbes, predominately bacteria, that harbor gastrointestinal tract. Changes in gut deviate from native composition, is, “dysbiosis,” proposed as probable diagnostic biomarker risk factor for HCC. However, whether dysbiosis or consequence unknown. Methods: To better understand role HCC, mice deficient toll-like receptor 5 (TLR5, bacterial flagellin) model...
Cardiotonic steroids (CTSs) are NKA α-1 (Na+/K+-ATPase α-1) ligands that increased in volume expanded states and associated with cardiac renal diseases. Although initiation resolution of inflammation is an important component cellular injury repair disease, it unknown whether CTS activation signaling this setting regulates inflammatory response. On background, we hypothesized through the α-1-Src kinase complex promotes a proinflammatory response epithelial immune cells. First, observed...
Three patients developed massive proteinuria, hypoalbuminemia, and edema after transplantation. These findings occurred in the immediate post-transplant period two patients, renal failure developed. The third patient proteinuria 4 months post-transplantation. There was complete remission of recovery function one. Renal histology similar all. Light microscopy demonstrated that glomeruli contained a mild increase mesangial matrix, but were otherwise normal. No significant interstitial cellular...
Aspergillosis is a commonly diagnosed fungal infection. Histopathologic examination alone can have diagnostic pitfalls due to the overlapping of morphology. We report case Scedosporium boydii infection initially misdiagnosed as aspergillosis. The patient presented hospital with shortness breath and chest abdominal pain. Laboratory tests revealed leukocytosis elevated serum liver enzymes, myoglobin lipase. He died hypotensive shock brain abscesses despite antibiotic treatment. Autopsy...