A5102972333- Gut microbiota and health
- Diet and metabolism studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Research on Leishmaniasis Studies
- Tryptophan and brain disorders
- Iron Metabolism and Disorders
- Immune cells in cancer
- Trace Elements in Health
- Inflammatory Bowel Disease
- Metabolomics and Mass Spectrometry Studies
- Trypanosoma species research and implications
- Immune Response and Inflammation
- Malaria Research and Control
- Liver Disease Diagnosis and Treatment
- Digestive system and related health
- Drug Transport and Resistance Mechanisms
- Immune Cell Function and Interaction
- Dietary Effects on Health
- Salmonella and Campylobacter epidemiology
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Diet, Metabolism, and Disease
- IL-33, ST2, and ILC Pathways
- Metabolism and Genetic Disorders
- Gastrointestinal motility and disorders
University of Toledo
2018-2025
Pennsylvania State University
2016-2017
National Institute of Cholera and Enteric Diseases
2014-2016
Institute of Post Graduate Medical Education and Research
2006-2013
Bose (United States)
2008-2012
Bose Institute
2007
Diets rich in fermentable fibres provide an array of health benefits; however, many patients with IBD report poor tolerance to fibre-rich foods. Intervention studies dietary murine models colonic inflammation have yielded conflicting results on whether ameliorate or exacerbate IBD. Herein, we examined how replacing the insoluble fibre, cellulose, fibres, inulin pectin, impacted colitis resulting from immune dysregulation via inhibition interleukin (IL)-10 signalling and/or innate deficiency...
A major impediment to effective anti-leishmanial chemotherapy is the emergence of drug resistance, especially sodium antimony gluconate, first-line treatment for leishmaniasis. Artemisinin, a sesquiterpene lactone isolated from Artemisia annua, an established anti-malarial compound that showed activity in both promastigotes and amastigotes, with IC(50) values 160 22 microM, respectively, and, importantly, was accompanied by high safety index (>22-fold). The leishmanicidal artemisinin...
Numerous studies signify that diets rich in phytochemicals offer many beneficial functions specifically during pathologic conditions, yet their effects are often not uniform due to inter-individual variation. The host indigenous gut microbiota and modifications of dietary have emerged as factors greatly influence the efficacy phytoceutical-based intervention. Here, we investigated biological activities one such active microbial metabolite, Urolithin A (UA or...
Lipocalin 2 (Lcn2) is a multifunctional innate immune protein whose expression closely correlates with extent of intestinal inflammation. However, whether Lcn2 plays role in the pathogenesis gut inflammation unknown. Herein, we investigated to which regulates and bacterial dysbiosis mouse models IBD.Lcn2 was monitored murine colitis upon microbiota ablation/restoration. WT knockout (Lcn2KO) mice were analyzed for load, composition by 16S rRNA gene pyrosequencing and, their colitogenic...
Commensal gut microbiota are strongly correlated with host hemodynamic homeostasis but only broadly associated cardiovascular health. This includes a general correspondence of quantitative and qualitative shifts in intestinal microbial communities found hypertensive rat models human patients. However, the mechanisms by which microbes contribute to function organs important for blood pressure (BP) control remain unanswered. To examine direct effects on BP, we conventionalized germ-free (GF)...
Hepatocellular carcinoma (HCC) is a male-dominant disease, but targeted sex hormone therapies have not been successful. Bile acids are potential liver carcinogen and biomolecules with hormone-like effects. A few studies highlight their dimorphism in physiology disease. We hypothesized that bile could be molecular signature explains disparity HCC. used the farnesoid X receptor knockout (FxrKO) mouse model to study acid-dependent Temporal tracking of circulating determined more than 80% FxrKO...
Neutrophils are the primary immune cells that respond to inflammation and combat microbial transgression. To thrive, bacteria residing in their mammalian host have withstand antibactericidal responses of neutrophils. We report enterobactin (Ent), a catecholate siderophore expressed by Escherichia coli, inhibited PMA-induced generation reactive oxygen species (ROS) neutrophil extracellular traps (NETs) mouse human Ent also impaired degranulation granules phagocytosis bactericidal activity...
Intestinal contents comprise the largest repository of immunogenic ligands microbial origin. We undertook this study to assess predominant microbe-associated molecular patterns (MAMPs) present therein and receptors) that mediate innate immune responses them. Here, we demonstrated intestinal from conventional, but not germ-free, mice rats triggered robust in vitro vivo. Such were abrogated absence either myeloid differentiation factor 88 (MyD88) or Toll-like receptor (TLR) 5, TLR4, suggesting...
Background A complex interplay between Leishmania and macrophages influences parasite survival necessitates disruption of signaling molecules, eventually resulting in impairment macrophage function. In this study, we demonstrate the immunomodulatory activity Berberine chloride infected macrophages. Principal Findings The IC50 chloride, a quaternary isoquinoline alkaloid was tested an amastigote model its safety index measured by cell viability assay. It eliminated intracellular amastigotes,...
Abstract Nitric oxide (NO) has been demonstrated to be a principal effector molecule responsible for mediating intracellular killing of Leishmania parasites, the causative organism leishmaniasis. As measurement NO remains challenge biologists, we have developed flow cytometric approach perform real time biological detection within parasites and parasitized macrophages using membrane permeable derivative diaminofluorescein [4,5‐diaminofluorescein diacetate (DAF‐2DA)]. Initially, assay...
Enterobactin (Ent), a prototypical bacterial siderophore known for its unparalleled affinity iron, is widely conserved among members of the
Alterations of diurnal rhythms blood pressure (BP) and reshaping gut microbiota are both independently associated with hypertension. However, the relationships between biorhythms BP microbial composition unknown. We hypothesized that timing-associated alterations compositions synchronous rhythmicity, dip in BP, renal function. To test this hypothesis, Dahl salt-sensitive (S) rats on low- high-salt diets were examined for time day effects microbiota, indicators damage. Major shifts night...
Background: Hypertension is the largest risk factor affecting global mortality. Despite available medications, uncontrolled hypertension on rise, whereby there an urgent need to develop novel and sustainable therapeutics. Because gut microbiota now recognized as important entity in blood pressure regulation, one such new avenue target gut-liver axis wherein metabolites are transacted via host-microbiota interactions. Knowledge which within regulate largely unknown. Method: To address this,...