Ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation mRNA leading to protein synthesis. However, certain ribosomal moonlight development and differentiation organisms. In this study, L44 (RPL44), associated with salt resistance, was screened from halophilic fungus Aspergillus glaucus (AgRPL44), its activity investigated Saccharomyces cerevisiae Nicotiana tabacum. Sequence alignment revealed that AgRPL44 is one large subunit...

Engineering high-fidelity CRISPR enzymes often leads to reduced cleavage activity, creating a significant hurdle in balancing nuclease specificity and efficiency for clinical applications. Here, we demonstrate that extending the spacer 21 or 22 nucleotides restores impaired activity of SuperFi-Cas9, an engineered Cas9 variant with seven mutations at PAM-distal region. Structural mutational analyses reveal extension strengthens additional interactions end, stabilizing nuclease-sgRNA-DNA...

High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering enhancing tolerance being tested as an efficient and viable strategy improvement. We previously characterized a large subunit ribosomal protein RPL44, which involved osmotic stress extremely halophilic fungus Aspergillus glaucus. Here, we screened another (AgRPS3aE) also produced high-salt...

The shrub Chimonanthus praecox L. (wintersweet) which is native to Chinese montane forests produces its flowers in the midst of winter. This indicates that floral organs this species are adapted growth and development under freezing temperatures. Here, we report isolation preliminary characterisation a 33 kDa apoplastic antifreeze chitinase (CpCHT1) from petals corresponding cDNA. activity CpCHT1 was confirmed by staining. Antifreeze validated terms formation bipyramidal ice crystals high...

Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1, which made it an attractive resource for gene cloning. This research a Glycosyl Hydrolase (Tk-ChiA) form T. Kodakarensis with dual hydrolytic activity due to presence of three binding domains affinity towards chitin and cellulose. The Tk-ChiA was cloned expressed on Pichia pastoris GS115. molecular weight purified is about 130.0 kDa. By using Chitosan, CMC-Na other...

Dihydroxynapthalene-(DHN) and L-dihydroxyphenylalanine (L-DOPA) are two types of dominant melanin in fungi. Fungal melanins with versatile functions frequently associated pathogenicity stress tolerance. In rice blast fungus, Magnaporthe oryzae, DHN is essential to maintain the integrity infectious structure, appressoria; but role tyrosinase-derived L-DOPA still unknown. Here, we have genetically biologically characterized a tyrosinase gene (MoTyr) M. oryzae. MoTyr encodes protein 719 amino...

ABSTRACT Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation myriad species. Fungal species form a large group of eukaryotic organisms, their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal have been biologically investigated. Here, glpF gene from the halophilic fungus Aspergillus glaucus ( AgglpF ) was verified to channel or Xenopus laevis oocytes further functionally analyzed...

Summary The ATP ‐dependent Lon protease is involved in many physiological processes. In bacteria, regulates pathogenesis and, yeast, protects mitochondia from oxidative damage. However, little known about fungal phytopathogens. MAP1 , a homologue of M agnaporthe oryzae was recently identified to be important for stress resistance and pathogenesis. Here, we focus on novel pathogenic pathway mediated by . Based an interaction system between rice tandem affinity purification ( TAP )‐tagged...

Rice blast caused by Magnaporthe oryzae is one of the most serious rice diseases worldwide. Biological control gaining popularity as a promising method for this disease; however, more effective microbial strains with strong adaptability in fields need to be identified. Here, we report first time successful identification biocontrol bacterial from frozen soils soda saline-sodic land. We isolated 82 western Songnen Plain China, three major saline world. Five exhibited inhibition M. growth. The...

ABSTRACT Large herbivore grazing plays a key role in regulating ecosystem functioning and nutrient cycling terrestrial ecosystems. However, the effects mechanisms of large intensity on soil fractions remain largely unclear alpine grasslands. Here, we determine 20 indicator variables associated with (e.g., particulate organic carbon, POC; mineral‐associated MAOC) to investigate how labile through well‐controlled experiment an meadow eastern Tibetan Plateau. Our results show that microbial...

Abstract Background The rice blast is a typical fungal disease caused by Magnaporthe oryzae , and the mitochondrial ATP-dependent Lon protease (MAP1) has been proven to be involved in development. We previously screened C3HC type Zinc-finger domain protein (ZFC3), which interacted with MAP1. purpose of this research was study biological function ZFC3 M. . Results first confirmed that ZFC3-RFP fusion localized within mitochondria. deleted mutant strains ( ∆ZFC3 ) showed enhanced expression...

Human PTEN, a dual-phosphatase tumor suppressor, is frequently dysregulated by alternative splicing. Fungi harbor PTEN homologs, but splicing of fungal PTENs has not been reported as far we know. Here, described an case in the homolog Magnaporthe oryzae ( MoPTEN ). Two splice variants were detected and identified, which are resulted from intron retention exclusion MoPTEN-1/2 Both proteins different lipid protein phosphatase activity expression patterns. The deletion mutant (Δ ) showed...

Bicarbonate (HCO3-) transporter family including the anion exchanger (AE) group is involved in multiple physiological processes through regulating acid-base homeostasis. HCO3- transporters have been extensively studied mammals, but fungal homologues of AE are poorly understood. Here, we characterized member (MoAE4) Magnaporthe oryzae. MoAE4 exhibits more sequence and structure homologies with reported AE4 BOR1 proteins. In addition to common sublocalization on cytomembrane, also localizes...

Serendipita indica, a multifunctional and useful endophyte fungus, has been intensively investigated in promoting plant growth resistance towards biotic abiotic stress. Multiple chitinases from microorganisms or plants have identified to high antifungal activity as biological control. However, chitinase of S. indica still needs be characterized. We functionally characterized (SiChi) indica. The result showed that the purified SiChi protein confers activity; importantly, inhibits conidial...

Aspartate family includes five additional amino acids other than aspartate, among which most except aspartate have been reported for their action in pathogenesis by acid biosynthesis. However, how the initial substrate of this metabolic pathway, is involved remains unknown. Here, we focused on transaminase (AST) that catalyzes transamination reaction between glutamate-aspartate Magnaporthe oryzae. Three MoAST genes were bioinformatically analyzed, MoAST2 was uniquely upregulated when...

The antifungal mechanism of mycoparasitic fungi involves fungal cell wall degrading enzymes such as chitinases. Trichothecium roseum is an important fungus with significant ability, but studies on chitinases T. were poor. Here, we report a novel chitinase cDNA isolated from by PCR amplification based conserved sequences. Southern blot analysis suggested that single copy the gene exists in genome roseum. deduced open reading frame 1,143 nucleotides encodes protein 380 amino acids calculated...

Fungal sexual development requires the involvement of a large number functional genes. genes encoding differentiation process proteins (SDPs), isps, have been known for decades. isp4/SDP and its homologs function as oligopeptide transporters (OPTs), yet their roles in reproduction are unknown. Here, we genetically analyzed all four species Chaetomium thermophilum asexual Thermomyces lanuginosus. Using single gene deletion mutants, found that T. lanuginosus SDP (TlSDP) participated...

Summary ATP‐dependent Lon proteases function in bacterial pathogenesis by regulating the expression of Type III secretion system; however, little is known about how regulate fungal pathogenesis. We previously investigated Lon‐binding proteins involved that interact with PrePL, smallest Magnaporthe oryzae protein. Here, we show cleaves PrePL and produces Pc, an extracellular 11‐kDa isoform catalase peroxidase activity. The Δ loss‐of‐function strain showed stronger sporulation accelerated...