Construction of catalytic centers on natural protein aggregates is a challenging topic in biomaterial and biomedicine research. Here we report novel construction artificial nanoenzyme with glutathione peroxidase (GPx)-like function. By engineering the surface tobacco mosaic virus (TMV) coat protein, main components GPx were fabricated TMV monomers. Through direct self-assembly functionalized viral proteins, multi-GPx installed these well-defined nanodisks or nanotubes. With help...

Ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation mRNA leading to protein synthesis. However, certain ribosomal moonlight development and differentiation organisms. In this study, L44 (RPL44), associated with salt resistance, was screened from halophilic fungus Aspergillus glaucus (AgRPL44), its activity investigated Saccharomyces cerevisiae Nicotiana tabacum. Sequence alignment revealed that AgRPL44 is one large subunit...

Tobacco mosaic virus (TMV) is a major threat to crops, making the discovery of green biopesticides essential. Herein, we present two active ingredients derived from medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibitors. Among them, 2 inhibited infestation on tobacco leaves at rate 38.6%, which was close that commercial antiviral agent ningnanmycin (43.1%). Both 1 could effectively alleviate destruction leaf...

The intercellular movement of plant viruses requires both viral and host proteins. Previous studies have demonstrated that the frame-shift protein P3N-PIPO (for encoded by open reading frame [ORF] containing 5'-terminus P3 a +2 ORF called Pretty Interesting Potyviridae embedded in P3) CYLINDRICAL INCLUSION (CI) proteins were required for potyvirus cell-to-cell movement. Here, we provide genetic evidence showing Tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) mutant carrying...

Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic plant pathogen with worldwide distribution. The sclerotia of S. are pigmented multicellular structures formed from the aggregation vegetative hyphae. These survival play central role in life and infection cycles this pathogen. Here, we characterized an atypical forkhead (FKH)-box-containing protein, SsFKH1, involved sclerotial development virulence. To investigate SsFkh1 sclerotiorum, partial sequence was cloned RNA interference...

The plant pathogenic fungus Sclerotinia sclerotiorum can survive on a wide range of hosts and cause significant losses crop yields. FKH, forkhead box (FOX)-containing protein, functions to regulate transcription signal transduction. As factor (TF) with multiple biological in eukaryotic organisms, little research has been done the role FKH protein fungi. SsFkh1 encodes which predicted contain FOX domain S. sclerotiorum. In this study, deletion mutant resulted severe defects hyphal...

MADS-box proteins, a well-conserved family of transcription factors in eukaryotic organisms, specifically regulate wide range cellular functions, including primary metabolism, cell cycle, and identity. However, little is known about roles the protein fungal pathogen Sclerotinia sclerotiorum. In this research, S. sclerotiorum gene SsMADS was cloned; it encodes that highly similar to Mcm1 orthologs from Saccharomyces cerevisiae other fungi, includes conserved DNA-binding domain. MADS member...

Increasing evidence implicates long noncoding RNAs (lncRNAs), a class of >200 nucleotides in length, the development cancer. However, mechanism underlying effects lncRNAs prostate cancer (PCa) remains to be elucidated. The present study aimed investigate role lncRNA‑THBS4‑003 pathogensis PCa. In study, microarray containing 8,277 lncRNA probes and 32,207 mRNA were used identify dysregulated mRNAs three patients with PCa, reverse transcription‑quantitative polymerase chain reaction was...

SFH1 (for Snf5 homolog) protein, comprised in the RSC (Remodels Structure of Chromatin) chromatin remodeling complex, functions as a transcription factor (TF) to specifically regulate gene and remodeling. As one well-conserved TFs eukaryotic organisms, little is known about roles protein filamentous fungi. In Sclerotinia sclerotiorum, notorious plant fungal pathogens, there are nine proteins predicted contain GATA-box domain according GATA family TF classification, among which Sssfh1...

Sclerotinia sclerotiorum is a necrotrophic phytopathogenic fungus that cross-talks with its hosts for control of cell-death pathways colonization. Target rapamycin (TOR) central regulator controls cell growth, intracellular metabolism, and stress responses in variety eukaryotes, but little known about TOR signaling S. sclerotiorum. In this study, we identified conserved pathway characterized SsTOR as critical component pathway. Hyphal growth was retarded by silencing SsTOR, moreover,...

Sclerotinia sclerotiorum is a challenging agricultural pathogen for management, causing large global economic losses annually. The sclerotia and infection cushions are critical its long-term survival successful penetration on wide spectrum of hosts. mitogen-activated protein kinase (MAPK) cascades serve as central signaling complexes that involved in various aspects development infection. In this study, the putative downstream transcription factor MAPK pathway, SsSte12, was analyzed S....

Peroxiredoxins (Prxs) act against hydrogen peroxide (H2O2), organic peroxides, and peroxynitrite. Thermococcus kodakaraensis KOD1, an anaerobic archaeon, contains many antioxidant proteins, including three Prxs (Tk0537, Tk0815, Tk1055). Only Tk0537 has been found to be induced in response heat, osmotic, oxidative stress. was belong a 1-Cys Prx6 subfamily based on sequence analysis named TkPrx. Using gel filtration chromatography, electron microscopy, blue-native polyacrylamide...

AMS2, a multicopy suppressor for the cpn1 (SpCENP-A) mutant, functions to specifically regulate histone genes transcription and chromosome segregation. As cell-cycle-regulated GATA factor in eukaryotic organisms, little research has been done on role of AMS2 protein pathogenic fungi. In Sclerotinia sclerotiorum, Ssams2 (SS1G_03252) encodes which predicted contain GATA-box domain. Here, Ssams2-silenced strains with significantly reduced gene expression levels exhibited defect hyphal growth,...

Sclerotinia sclerotiorum is a widely dispersed plant pathogenic fungus causing many diseases such as white mold, stem rot, stalk and head rot on varieties of broadleaf crops worldwide. Previous studies have shown that the Forkhead-box transcription factors (FOX TFs) play key regulatory roles in sexual reproduction some fungi. Ss-FoxE2 one four FOX TF family member genes S. sclerotiorum. Based ortholog function other fungi it hypothesized to reproduction. In this study, role was identified...

Abstract Sclerotinia sclerotiorum , the notorious necrotrophic phytopathogenic fungus with wide distribution, is responsible for sclerotium disease in more than 600 plant species, including many economic crops such as soybean, oilseed rape, and sunflower. The compound appressorium a crucial multicellular infection structure that prerequisite infecting healthy tissues. Previously, Forkhead‐box family transcription factors (FOX TFs) SsFoxE2 SsFKH1 were shown to play key regulatory role hyphae...

The thermophilic species, Thermococcus kodakarensis KOD1, a model microorganism for studying hyperthermophiles, has adapted to optimal growth under conditions of high temperature and salinity. However, the environmental strain are not always stable, this might face different stresses. In present study, we compared proteome response T. heat, oxidative, salt stresses using two-dimensional electrophoresis, protein spots were identified through MALDI-TOF/MS. Fifty-nine, forty-two, twenty-nine...

Background The RhoA/ROCK pathway and Caveolin-1 (Cav-1) participate in the process of tumorigenesis numerous types cancer. Up-regulation Cav-1 expression is considered to be associated with development progression clear cell renal carcinoma (ccRCC). We investigated association between genetic variations risk ccRCC Chinese population. Methods Between May 2004 March 2014, a total 1,248 cases 1,440 cancer-free controls were enrolled this hospital-based case-control study. Nine SNPs genotyped...

GATA transcription factors (TFs) are common eukaryotic regulators, and glutathione-dependent formaldehyde dehydrogenases (GD-FDH) ubiquitous enzymes with detoxification activity. In this study, the dehydrogenase Sclerotinia sclerotiorum Fdh1 (SsFdh1) was first characterized as an interacting partner of a TF, SsNsd1, in S. Genetic analysis reveals that SsFdh1 functions detoxification, nitrogen metabolism, sclerotium development, pathogenicity. Both SsNsd1 harbor typical zinc finger motifs...