A5100416434- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Insect Resistance and Genetics
- Peanut Plant Research Studies
- Transgenic Plants and Applications
- Cocoa and Sweet Potato Agronomy
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Plant Pathogens and Resistance
- Plant Disease Management Techniques
- Legume Nitrogen Fixing Symbiosis
- Agronomic Practices and Intercropping Systems
- RNA Research and Splicing
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Mycorrhizal Fungi and Plant Interactions
- Fungal Plant Pathogen Control
- Genomics and Phylogenetic Studies
Shandong Agricultural University
2016-2025
Shandong Academy of Agricultural Sciences
2023-2025
Institute of Plant Protection
2014-2025
Capital Normal University
2024
State Key Laboratory of Crop Biology
2020
Agilent Technologies (United States)
2020
State Council of the People's Republic of China
2019
Municipality of Tai'an
2019
China Tobacco
2018
Chinese Academy of Agricultural Sciences
2018
ABSTRACT Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors small interfering (siRNA) or microRNA, respectively, thereby initiating amplifying silencing-based antiviral defense gene regulation in eukaryotic cells. However, we now provide evidence that a class 1 is involved suppression of silencing. The single-stranded genome sweet potato chlorotic stunt virus (SPCSV) encodes an (RNase3) homologous putative IIIs unknown function...
Lipidomics has great promise in various applications; however, a major bottleneck lipidomics is the accurate and comprehensive annotation of high-resolution tandem mass spectral data. While number available software drastically increased over past five years, reduction false positives realization obtaining structurally annotations remains significant challenge. We introduce Lipid Annotator, which user-friendly for lipidomic analysis data collected by liquid chromatography spectrometry...
Stalk rot caused by Fusarium verticillioides (Fv) is one of the most destructive diseases in maize production. The defence response root system to Fv invasion important for plant growth and development. Dissection cell type-specific infection its underlying transcription regulatory networks will aid understanding mechanism roots invasion. Here, we reported transcriptomes 29 217 single cells derived from tips two inbred lines inoculated with mock condition, identified seven major types 21...
Continuous monocropping of peanuts (Arachis hypogaea L.) often results in yield decline and soil degradation. The combination green manure (GM) with tillage practices has been proposed as a sustainable strategy to maintain high crop productivity improve quality. This study investigates the long-term effects 8 years GM application combined plow on microbial communities physicochemical properties under peanut system. Treatments included: (i) no (NT); (ii) before winter fallow period (PT);...
Agrotis ipsilon (Lepidoptera: Noctuidae) is a major underground pest that damages many agricultural crops in China and other countries. A diet-incorporation-based bioassay was conducted to evaluate the sublethal effects of novel anthranilic diamide chlorantraniliprole on nutritional physiology, enzymatic properties population parameters this cutworm. Chlorantraniliprole exhibited signs active toxicity against third instar larvae A. ipsilon, LC
Cucurbits are economically important crops worldwide. The genomic data of many cucurbits now available. However, functional analyses cucurbit genes and noncoding RNAs have been impeded because genetic transformation is difficult for cucurbitaceous plants. Here, we developed a set tobacco ringspot virus (TRSV)-based vectors gene microRNA (miRNA) function studies in cucurbits. A TRSV-based expression vector could simultaneously express GREEN FLUORESCENT PROTEIN (GFP) heterologous viral...
Trichomes, the hair-like structures located on aerial parts of most vascular plants, are associated with a wide array biological processes and affect economic value certain species. The involved in unicellular trichome formation have been well-studied Arabidopsis (Arabidopsis thaliana). However, our understanding morphological changes underlying molecular multicellular development is limited. Here, we studied dynamic developmental glandular nonglandular cucumber (Cucumis sativus L.) divided...
Abstract Maize chlorotic mottle virus (MCMV) is the key pathogen causing maize lethal necrosis (MLN). Due to sharply increased incidence of MLN in many countries, there an urgent need identify resistant lines and uncover underlying resistance mechanism. Here, we showed that abundance (Zea mays) microR167 (Zma-miR167) positively modulates degree MCMV. Zma-miR167 directly targets Auxin Response Factor3 (ZmARF3) ZmARF30, both which negatively regulate RNA-sequencing coupled with gene expression...
Mosaic symptoms are commonly observed in virus-infected plants. However, the underlying mechanism by which viruses cause mosaic as well key regulator(s) involved this process remain unclear. Here, we investigate maize dwarf disease caused sugarcane virus (SCMV). We find that manifestation of SCMV-infected plants requires light illumination and is correlated with mitochondrial reactive oxidative species (mROS) accumulation. The transcriptomic metabolomic analyses results together genetic...
The eukaryotic mRNA surveillance pathway, a pivotal guardian of fidelity, stands at the nexus diverse biological processes, including antiviral immunity. Despite recognized function splicing factors on fate, intricate interplay shaping pathway remains elusive. We illustrate that conserved factor U2 snRNP auxiliary large subunit B (U2AF65B) modulates complex, contributing to transcriptomic homeostasis in maize. functionality requires ZmU2AF65B-mediated normal upstream frameshift 3 ( ZmUPF3 )...
Tomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses severe threat to the tomato industry. So far, it has spread 10 countries America, Asia, Europe. In 2019, ToBRFV was identified Shandong Province (ToBRFV-SD), China. this study, shown that ToBRFV-SD induced mild mosaic blistering on leaves, necrosis sepals pedicles, deformation, yellow spots, necrotic lesions fruits. distinct symptoms plants of tomato, Capsicum annumm, Nicotiana benthamiana,...
Abstract Tomato cultivars containing the Tm‐2 2 resistance gene have been widely known to resist tobacco mosaic virus (TMV) and tomato virus. brown rugose fruit (ToBRFV), a new emerging tobamovirus, can infect plants carrying gene. However, virulence determinant of ToBRFV that overcomes conferred by remains unclear. In this study, we substituted movement protein (MP) encoding sequences between TMV infectious clones conducted infectivity assays. The results showed MP was for transgenic...
Eukaryotic translation initiation factor 4E (eIF4E), which plays a pivotal role in initiating eukaryotic organisms, is often hijacked by the viral genome-linked protein to facilitate infection of potyviruses. In this study, we found that naturally occurring amino acid substitution D71G eIF4E widely present potyvirus-resistant watermelon accessions and disrupts interaction between papaya ringspot virus-watermelon strain, zucchini yellow mosaic virus or virus. Multiple sequence alignment...
Papain-like cysteine proteases (PLCPs) play pivotal roles in plant defense against pathogen invasions. While pathogens can secrete effectors to target and inhibit PLCP activities, the of PLCPs plant-virus interactions mechanisms through which viruses neutralize activities remain largely uncharted. Here, we demonstrate that expression activity a maize CCP1 (Corn Cysteine Protease), is upregulated following sugarcane mosaic virus (SCMV) infection. Transient silencing led reduction thereby...
Viruses exploit autophagy to degrade host immune components for their successful infection. However, how viral factors sequester the autophagic substrates into autophagosomes remains largely unknown. In this study, we showed that p20 protein, a suppressor of RNA silencing (VSR) encoded by citrus tristeza virus (CTV), mediated degradation SUPPRESSOR OF GENE SILENCING 3 (SGS3), plant-specific RNA-binding protein is pivotal in antiviral silencing. CTV infection activated autophagy, and...
Abstract Stochastic resonance can utilize the energy of noise to enhance weak frequency characteristic. This paper proposes an adaptive multi-stable stochastic method assisted by neural network (NN) and physics supervision (directly numerical simulation physical system). Different from traditional algorithm, evaluation objective function (i.e., fitness function) in iteration process algorithm is through a trained instead simulation. It will bring dramatically reduction computation time....
The intercellular movement of plant viruses requires both viral and host proteins. Previous studies have demonstrated that the frame-shift protein P3N-PIPO (for encoded by open reading frame [ORF] containing 5'-terminus P3 a +2 ORF called Pretty Interesting Potyviridae embedded in P3) CYLINDRICAL INCLUSION (CI) proteins were required for potyvirus cell-to-cell movement. Here, we provide genetic evidence showing Tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) mutant carrying...
Cyantraniliprole is a novel diamide insecticide that acts upon the ryanodine receptor (RyR) and has broad application prospects. Accordingly, it very important to evaluate toxicity of cyantraniliprole earthworms (Eisenia fetida) because their vital role in maintaining healthy soil ecosystem. In this study, an experiment was set up, using four concentrations (0.1, 1, 5, 10 mg/kg) solvent control group (0 mg/kg), investigate ecotoxicity earthworms. Our results showed that, after 28 days...
Abstract Tobacco mosaic virus (TMV; genus Tobamovirus ) is one of the most prevailing pathogens that seriously affects quality and yield tobacco ( Nicotiana tabacum leaves. Cross-protection using mild strains a potential strategy for biological prevention plant viral diseases. Complementary mutations in attenuated may cause ones to suddenly evolve into virulent strains, which limits application cross-protection practice. To data there has been no study on engineering complementary mutation...
Chloroplast-bound vesicles are key components in viral replication complexes (VRCs) of potyviruses. The potyviral VRCs induced by the second 6 kDa protein (6K2) and contain at least RNA nuclear inclusion b. To date, no chloroplast has been identified to interact with 6K2 involve potyvirus replication. In this paper, we showed that Photosystem II oxygen evolution complex Nicotiana benthamiana (NbPsbO1) was a interacting Tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) present VRCs....