A5066663413- Cervical Cancer and HPV Research
- Thyroid Cancer Diagnosis and Treatment
- Reproductive System and Pregnancy
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- Head and Neck Cancer Studies
- AI in cancer detection
- Glaucoma and retinal disorders
- Immune Response and Inflammation
- Ocular Surface and Contact Lens
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Women's cancer prevention and management
- Education Pedagogy and Practices
- Radiomics and Machine Learning in Medical Imaging
- Cancer Diagnosis and Treatment
- Research on Leishmaniasis Studies
- Cancer-related Molecular Pathways
- Esophageal Cancer Research and Treatment
- Hepatitis B Virus Studies
- Autopsy Techniques and Outcomes
- Health, Nursing, Elderly Care
- Education and Public Policy
- Education and Digital Technologies
- Laser Applications in Dentistry and Medicine
Universidade de São Paulo
2011-2024
Universidade de Ribeirão Preto
2009-2023
Fundação Faculdade de Medicina
2017
Clinics Hospital of Ribeirão Preto
1988-2015
Universidade Federal de São Carlos
2012
Universidade Federal de São Paulo
2009
Universidade Brasil
2007
Universidade Cidade de São Paulo
2001
Hospital Universitário da Universidade de São Paulo
1987
Abstract Background The greatest challenges in vaccine development include optimization of DNA vaccines for use humans, creation effective single-dose vaccines, delivery systems that do not involve live viruses, and the identification new adjuvants. Herein, we describe a novel, simple technique efficiently vaccinating mice against tuberculosis (TB). Our consists single-dose, genetic formulation DNA-hsp65 complexed with cationic liposomes administered intranasally. Results We developed novel...
Considering that downregulation of HLA expression could represent a potential mechanism for breast carcinogenesis and metastasis, the aim present study was to use immunohistochemical methods analyze HLA-Ia, HLA-DR, HLA-DQ, HLA-E, HLA-G in invasive ductal carcinoma (IDC) relate this profile anatomopathological parameters. Fifty-two IDC from biopsies were stratified according histological differentiation (well, moderately, poorly differentiated) presence metastases axillary lymph nodes. The...
HLA-G and HLA-E are two nonclassical class I molecules, which have been well recognized as modulators of innate adaptive immune responses, the expression these molecules in virus infected cells has associated with subversion response.In this study we performed a cross-sectional study, systematically comparing benign, pre-malignant malignant laryngeal lesions, correlating demographic clinical variables presence high-risk low-risk HPV types.Laryngeal lesions were collected from 109 patients...
A DNA vaccine based on the heat-shock protein 65 Mycobacterium leprae gene (pHSP65) presented a prophylactic and therapeutic effect in an experimental model of tuberculosis. In this paper, we addressed question which protective mechanisms are activated tuberculosis-infected mice after immune therapy with pHSP65. We evaluated activation cellular response lungs infected 30 days infection (initiation therapy) those uninfected mice. After 70 (end therapy), responses untreated mice,...
To study the possible effects of diclofenac sodium on intestinal anastomoses, 48 rabbits were submitted to surgery consisting two single-layer ileal anastomoses performed with separate propylene 5-0 sutures. The animals divided at random into groups (test and control). in test group given intramuscular injections dose 3 mg/kg body weight 24-hour intervals, control an identical amount 0.9 percent saline. sacrificed 3rd, 7th, 14th postoperative days for macroscopic evaluation peritoneal cavity...
Previous studies have indicated that cancer may be promoted and/or exacerbated by inflammation and infection. The cytokines produced activated innate immune cells stimulate tumor growth progression are considered as important components in this process. interleukin (IL)-23/T helper (Th)17 axis, which exerts marked pro-inflammatory effects, has emerged an mediator inflammation-associated cancer. Increasing clinical evidence indicates Th17 promote or inhibit progression, however, the function...
Human leukocyte antigen-G (HLA-G) is a non-classical major histocompatibility complex class Ib molecule that acts as specific immunosuppressor. Some studies have demonstrated human papillomavirus (HPV) seems to be involved in lower or absent HLA-G expression, particularly cervical cancer. In this study, we performed cross-sectional systematically comparing the qualitative expression of HLA-G5 isoform invasive carcinoma (ICC), stratifying patients according presence [ICC with metastasis...
HLA-G is a nonclassical major histocompatibility complex molecule that has well-recognized immunomodulatory properties. The expression of in tumor cells been considered to be detrimental, permitting spreading and decreased survival. We evaluated the histologically normal thyroid tissue, goiter, benign malignant tumors, studied relationship between patient clinical variables.The immunohistochemistry was performed on 72 specimens papillary carcinoma (PTC), 19 follicular carcinomas (FTC), 22...
Inactivation of the cell cycle inhibitor gene p16MTS1 seems to be involved in human papillomavirus (HPV)-related carcinogenesis because E6 and E7 oncoproteins may impair p16 INK4a and, indirectly, bcl-2 functions. In this study, we analyzed role immunohistochemical expression HPV-infected cervical biopsies as prognostic markers progression squamous intraepithelial lesion (SIL). Sixty-five were stratified into two subgroups according second biopsy: 27 them maintained a low-grade (LG)-SIL...
Vaccines are considered by many to be one of the most successful medical interventions against infectious diseases. But significant obstacles remain, such as optimizing DNA vaccines for use in humans or large animals. The amount doses, route and easiness administration also important points consider design new vaccines. Heterologous prime-boost regimens probably represent best hope an improved vaccine strategy. In this study, we have shown that heterologous vaccination tuberculosis (TB)...
As BRAF, TERT, HLA-G, and microRNAs have been individually associated with papillary thyroid carcinoma (PTC), we aimed to evaluate the individual collaborative role of these markers in PTC same patient cohort. HLA-G BRAF tumor expression was evaluated by immunohistochemistry. Using molecular methods, BRAFV600E TERT promoter mutations were fine needle aspirates. MicroRNA profiling investigated using massively parallel sequencing. We observed strong (67.96%) while (62.43%) staining specimens....
Study objective – The aim of the study was to evaluate role β receptor antagonists in evolution experimental Chagas’ disease. Design Rats were infected with T cruzi, 2000 parasites·g−1 body weight, soon after weaning. One group then given metoprolol, 100 mg·kg−1·d−1, drinking water. A comparison received no metoprolol. Two control groups non-infected rats also studied, one and without metoprolol same dose. Subjects Adult male albino used: 144 cruzi (group IM), 137 IW), 46 CM), 43 did not...
The larynx is the most common site of malignancy in upper aerodigestive tract. In Brazil, malignant laryngeal lesions represent 2% all cancers, with approximately 3000 annual deaths. association between human papillomavirus (HPV) and cancer still controversial. aim present retrospective study was to determine expression galectin-3 immunoperoxidase carcinoma by examining paraffin-embedded biopsies from 65 patients, 10 situ carcinomas, 27 carcinomas without metastases, 28 metastases....
Summary The results of various animal model studies tuberculosis (TB) suggest that culture filtrate proteins (CFPs), which are antigens secreted by Mycobacterium , largely responsible for improvements in TB vaccines. great obstacle to developing protein subunit vaccines is adjuvants required order stimulate relevant protective immune responses. Acting as adjuvants, CpG‐oligodeoxynucleotides (CpG‐ODNs) promote the activation Th1 cells and pro‐inflammatory cytokines. To evaluate adjuvant role...
Subclinical inflammation may be observed in patients using topical antiglaucomatous drugs. The objective of this study was to investigate conjunctiva glaucoma prostaglandin analogs, by the detection an immunogenetic marker (HLA-DR) and compare effect 3 different drugs: latanoprost, bimatoprost, travoprost induction inflammation.Thirty-three with primary open-angle were evaluated without analogs therapy. Imprints conjunctival cells obtained, fixed on glass slides, prepared for...
Rocio virus (ROCV) caused an outbreak of human encephalitis during the 1970s in Brazil and its immunopathogenesis remains poorly understood. CC-chemokine receptor 5 (CCR5) is a chemokine that binds to macrophage inflammatory protein (MIP-1 α). Both molecules are associated with cells migration infections. In this study, we demonstrated importance CCR5 MIP-1 α, outcome viral ROCV-infected mice. α knockout mice survived longer than wild-type (WT) animals. addition, had reduced inflammation...
Medullary thyroid carcinoma (MTC) occurs in a sporadic or as an autosomal dominant hereditary form. Inherited forms of MTC are related to mutations the RET proto-oncogene. We screened genomic DNA from 11 patients with for exons 10, 11, 13, 14, 15, and 16 Subsequently, we also evaluated family 1 patient presumed diagnosis MTC. Three MEN2A two unrelated families presented exon (C634Y C634R). A heterozygous mutation 14 (V804M) was identified observed different polymorphisms: S904S 15 (2...
We evaluated the use of a vaccine formulation based on mixture two different PLGA microspheres, composed by faster and slower release profiles, containing DNA encoding hsp65 recombinant protein, respectively, aiming to priming protein boost after single dose vaccination. The combination PLGA50:50 microspheres DNA-hsp65 trehalose dimycolate (TDM) with PLGA75:25 (prime-boost Me) was able induce high levels anti-hsp65 specific antibodies. serum these antibodies remained during 90 days...
Objective: The purpose of this study was to evaluate the effects low-level laser (LLL) energy on clinical signs inflammation and cellular composition synovial fluid (SF) in inflamed knee rabbit. Background Data: There are few findings related LLL SF inflammatory processes there is little knowledge about optimal parameters for reducing joint inflammation. Materials Methods: Inflammation right 36 rabbits induced by intracapsular injection (0.2 mL) Terebinthina commun (Tc). animals were...