A5100393808- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Neonatal Respiratory Health Research
- DNA Repair Mechanisms
- IL-33, ST2, and ILC Pathways
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Zebrafish Biomedical Research Applications
- Cancer Immunotherapy and Biomarkers
- Renal and related cancers
- Advanced battery technologies research
- Circular RNAs in diseases
- Cell death mechanisms and regulation
- Acute Kidney Injury Research
- NF-κB Signaling Pathways
- Collembola Taxonomy and Ecology Studies
- Bryophyte Studies and Records
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pluripotent Stem Cells Research
- Sepsis Diagnosis and Treatment
University of Chinese Academy of Sciences
2017-2024
Mengchao Hepatobiliary Hospital
2021-2024
Fujian Medical University
2021-2024
Fuzhou University
2021-2024
Lanzhou University
2024
Center for NanoScience
2024
National Center for Nanoscience and Technology
2024
Fujian Provincial Cancer Hospital
2024
Harbin Engineering University
2023
Center for Excellence in Molecular Cell Science
2010-2022
T cells play a critical role in tumor immunosurveillance by eliminating newly transformed somatic cells. However, cell variants can escape from immunological control after immunoediting, leading to progression. Whether and how respond growth remain unclear. Here, we found that tumor-infiltrating exhibited persistently up-regulated expression of the activator protein 1 (AP-1) subunit c-Fos during The ectopic exacerbated growth, whereas T-cell–specific deletion reduced malignancy. This...
Abstract mRNA vaccines are regarded as a highly promising avenue for next‐generation cancer therapy. Nevertheless, the intricacy of production, inherent instability, and low expression persistence linear significantly restrict their extensive utilization. Circular RNAs (circRNAs) offer novel solution to these limitations due efficient protein ability, which can be rapidly generated in vitro without need extra modifications. Here, we present neoantigen vaccine based on circRNA that induces...
Regulatory T (T reg) cells are required for the maintenance of immune homeostasis. Both TGF-β signaling and epigenetic modifications important Foxp3 induction, but how participates in regulation remains largely unknown. Here we showed that cell–specific ablation Uhrf1 resulted reg–biased differentiation TCR-stimulated naive absence signaling, these Foxp3+ had a suppressive function. Adoptive transfer Uhrf1−/− could significantly suppress colitis due to increased iT reg cell generation....
Disseminated intravascular coagulation (DIC) is a complication of sepsis currently lacking effective therapeutic options. Excessive inflammatory responses are emerging triggers coagulopathy during sepsis, but the interplay between immune system and not fully understood. Here we utilize murine model intraperitoneal lipopolysaccharide stimulation show neutrophils in circulation mitigate occurrence DIC, preventing subsequent septic death. We circulating release extracellular vesicles containing...
Significance Hematopoietic stem cells (HSCs) harbor the capacities of both self-renewal and differentiation to sustain life-long production all blood cells. However, how individual HSCs accomplish decision versus remains largely unknown. Here, we find that Uhrf1, a key epigenetic regulator DNA methylation, specifically controls this critical process. In absence undergo erythroid-biased at expense capacity, leading hematopoietic failure lethality. Mechanistically, Uhrf1 regulates HSC-division...
Intrathymic CD4/CD8 differentiation is a process that establishes the mutually exclusive expression profiles of CD4 and CD8 T cell lineage. The RUNX3-mediated silencing in lineage cells has been well documented; however, it unclear how silenced during differentiation. In this study, we report that, by directly binding locus, ThPOK works as negative regulator mediates deacetylation Cd8 genes repositions alleles close to heterochromatin development ectopic resulted increased recruitment...
Abstract Aberrant self-renewal of leukemia initiation cells (LICs) drives aggressive acute myeloid (AML). Here, we report that UHRF1, an epigenetic regulator recruits DNMT1 to methylate DNA, is highly expressed in AML and predicts poor prognosis. UHRF1 required for leukemogenesis by maintaining LICs. Mechanistically, directly interacts with Sin3A-associated protein 30 (SAP30) through two critical amino acids, G572 F573 its SRA domain, repress gene expression. Depletion or SAP30 derepresses...
Tight control of cell-cycle progression is critical for T-lymphocytes to function properly. Slfn1 (Schlafen1) has been reported play an important role in the establishment and maintenance quiescence T-lymphocytes. However, how accomplishes this remains poorly understood. In present study, we show that nuclear localization a prerequisite induce arrest, with DnaJB6, identified as new Slfn1-binding protein, playing pivotal process. chaperone protein DnaJ/Hsp (heat-shock protein) 40 family,...
Significance Recombination activating gene (RAG)-mediated variable, diversity, and joining [V(D)J] recombination is the hallmark of adaptive immunity that only exists in jawed vertebrates. The evolutionary origin RAG remains largely unknown although many hypotheses have been proposed. This study indicates a RAG1-like DNA fragment ( bfRAG1L ) from invertebrate amphioxus might encode functional central domain vertebrate core RAG1. We show (if translated) virus-related protein can degrade both...
Abstract T cell receptor (TCR) signaling is important for homeostasis and function. However, how surface TCR levels are regulated its biological significance on cells remains largely unknown. Here, we show that the cell-specific deletion of Arpc2, a component Arp2/3 complex, results in compromised peripheral homeostasis. complex-nucleated actin filaments essential maintaining by regulating + endosome trafficking resting state controlling polarization endosomes during immune synapse formation...
Highlights•Uhrf1 expression is upregulated at stage 1 during iNKT cell development•Loss of Uhrf1 leads to defective differentiation and increased apoptosis•Akt-mTOR axis attenuated in Uhrf1-deficient cellsActive•Akt rescues developmental defects miceSummaryUhrf1 (also known as Np95) a regulator DNA methylation histone ubiquitination plays an important role embryogenesis tumorigenesis. Here, we report that essential for invariant natural killer T (iNKT) development. We found was significantly...
The Th-inducing POK (Th-POK, also known as ZBTB7B or cKrox) transcription factor is a key regulator of lineage commitment immature T cell precursors. It yet unclear the physiological functions Th-POK besides helper differentiation. Here we show that restrictedly expressed in luminal epithelial cells mammary glands upregulated at late pregnancy and lactation. Lineage exerts distinct biological tissue-specific manner. not required for fate determination. Mammary gland morphogenesis puberty...
Intrathymic positive selection matches CD4-CD8 lineage differentiation to MHC specificity. However, it is unclear whether signals induce choice or simply select thymocytes of the appropriate lineage. To investigate this issue, we assessed undergoing for expression CD8 markers perforin and Runx3. Using both population-based single-cell RT-PCR analyses, found large subsets class II (MHC-II)-signaled expressing these genes within CD4+ 8+ 8(int), but not 8- populations signaling competent mice....
Abstract CD1d-dependent type I NKT cells, which are activated by lipid antigen, known to play important roles in innate and adaptive immunity, as a portion of II cells. However, the heterogeneity especially NKT-like remains largely unknown. Here, we report profiling (NK1.1 + CD3e ) cells livers from wild (WT), Jα18-deficient CD1d-deficient mice single-cell RNA sequencing. Unbiased transcriptional clustering revealed distinct cell subsets. The transcriptomic profiles identified well-known...
The transcription factor interferon regulatory 4 (IRF4) was originally found to be preferentially expressed in lymphoid cells and required for the function, differentiation, homeostasis of both mature T B lymphocytes. Recent studies have indicated that IRF4 is also involved early B-cell development. However, role intrathymic T-cell development remains unknown. In this study, we show upregulated TCR-signaled thymocytes predominantly CD4 single-positive (SP), but not CD8 SP, cells....
Invariant natural killer T cells (iNKT cells) are a specific subset of that recognize glycolipid antigens and upon activation rapidly exert effector functions. This unique function is established during iNKT cell development; the detailed mechanisms this process, however, remain to be elucidated. Here authors show deletion mediator subunit Med23 in CD4+CD8+ double positive (DP) thymocytes completely blocks development at stage 2. dysregulation accompanied by bias expression genes related...