A5100367377- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Adipose Tissue and Metabolism
- Cancer-related gene regulation
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Neurogenesis and neuroplasticity mechanisms
- Ubiquitin and proteasome pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Bone Tissue Engineering Materials
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- Neuroscience and Neuropharmacology Research
- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- Developmental Biology and Gene Regulation
- Bone Metabolism and Diseases
- Pancreatic function and diabetes
- Wnt/β-catenin signaling in development and cancer
- Hepatitis B Virus Studies
Zhongshan Hospital
1998-2025
Fudan University
2019-2025
State Key Laboratory of Genetic Engineering
2019-2025
Shandong Provincial QianFoShan Hospital
2024
Shandong First Medical University
2024
Central People's Hospital of Zhanjiang
2024
Guangdong Medical College
2024
Zhangjiagang First People's Hospital
2024
Soochow University
2024
Baoji City Central Hospital
2024
Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in mitogen-activated kinase pathway required for vulva development Caenorhabditis elegans . We generated sur2 −/− embryonic stem cells to analyze its function as mammalian component. Our results show forms subcomplex of two other subunits, TRAP/Med100 95. Knock-out prevents activation by E1A-CR3 kinase–regulated ETS transcription factor Elk-1, but not multiple factors. These imply specific...
The body temperature is considered a universal cue by which the master clock synchronizes peripheral clocks in mammals, but mechanism not fully understood. Here we identified two cold-induced RNA-binding proteins (RBPs), Cirbp and Rbm3, as important regulators for entrained circadian gene expression. depletion of or Rbm3 significantly reduced amplitudes core genes. PAR-CLIP analyses showed that 3′UTR binding sites were enriched near polyadenylation (PASs). Furthermore, shortened 3′UTR,...
Significance Plant adaptation in nature relies on the appropriate response to symbiotic and pathogenic microbes. Chitin is an important component of mycorrhizal fungi cell wall a major microbe-associated molecular pattern (MAMP), which can activate recognition receptors trigger plant immunity. How MAMP-triggered immunity suppressed by establish symbiosis remains elusive. Here, we found that CO4 (Chitotetraose) receptor OsMYR1 cannot only initiate signaling but also repress rice depleting...
To maintain healthy mitochondrial enzyme content and function, mitochondria possess a complex protein quality control system, which is composed of different endogenous sets chaperones proteases. Heat shock 60 (HSP60) one these molecular has been proposed to play pivotal role in the regulation folding prevention aggregation. However, physiological function HSP60 mammalian tissues not fully understood. Here we generated an inducible cardiac-specific knockout mouse model, demonstrated that...
The increasing insight into the molecular and cellular processes within angiogenic cascade assists in enhancing survival integration of engineered bone constructs. Copper-doped bioactive glass (Cu-BG) is now a potential structural component novel scaffolds implants used orthopedic dental repairs. However, it difficult for BG, especially micro-nano particles, to be printed still retain its biological activity ability biodegrade. Additionally, mechanisms copper-stimulating autocrine paracrine...
Abstract Protein arginine methyltransferase 5 (PRMT5) is the primary generating symmetric-dimethyl-arginine marks on histone and non-histone proteins. PRMT5 dysregulation implicated in multiple oncogenic processes. Here, we report that PRMT5-mediated methylation of protein kinase B (AKT) required for its subsequent phosphorylation at Thr308 Ser473. Moreover, pharmacologic or genetic inhibition abolishes AKT1 15 methylation, thereby preventing translocation to plasma membrane recruitment...
Background: Liver fibrosis affects millions of people worldwide without an effective treatment.Although multiple cell types in the liver contribute to fibrogenic process, hepatocyte death is considered be trigger.Multiple forms death, including necrosis, apoptosis, and necroptosis, have been reported co-exist diseases.Mixed lineage kinase domain-like protein (MLKL) terminal effector necroptosis pathway.Although has play important role a number diseases, function MLKL yet unraveled.Methods...
Abstract Attention-deficit hyperactivity disorder (ADHD) is a prevalent psychiatric with high heritability, while its etiology and pathophysiology remain unclear. Med23 subunit of the Mediator complex, key regulator gene expression by linking transcription factors to RNA polymerase II. The mutations are associated several brain diseases including microcephaly, epilepsy intellectual disability, but biological roles in development possible behavioral consequence have not been explored animal...
Abstract How lineage specifiers are regulated during development is an outstanding question, and the molecular regulation of osteogenic factor RUNX2 remains to be fully understood. Here we report that Mediator subunit MED23 cooperates with regulate osteoblast differentiation bone development. Med23 deletion in mesenchymal stem cells or precursors results multiple defects similar those observed Runx2 +/− mice. In vitro , -deficient progenitor refractory differentiation, deficiency reduces...
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Abstract The aortic dissection (AD) is a life-threatening disease. transcatheter endovascular repair (EVAR) affords minimally invasive technique to save the lives of these critical patients, and an appropriate stent-graft gets be key medical device during EVAR procedure. Herein, we report trilayer corresponding delivery system used for treatment AD made nitinol stents with asymmetric Z-wave design two expanded polytetrafluoroethylene (ePTFE) membranes. Each inner outer surfaces was covered...
The circadian clock confers daily rhythmicity to many crucial biological processes and behaviors its disruption is closely associated with carcinogenesis in several types of cancer.Brain muscle arnt-like protein 1 (BMAL1) a core rhythm component mammals dysregulation has been shown contribute aberrant metabolism human diseases.However, the functions BMAL1, especially involvement lipid hepatocellular carcinoma (HCC), remain elusive.In present study, we found that BMAL1 was frequently...
Expanded polytetrafluoroethylene (ePTFE) is promising in biomedical fields such as covered stents and plastic surgery owing to its excellent biocompatibility mechanical properties. However, ePTFE material prepared by the traditional biaxial stretching process with thicker middle thinner sides due bowing effect, which poses a major problem industrial-scale fabrication. To solve this problem, we design an olive-shaped winding roller provide part of tape greater longitudinal amplitude than two...
4092 Background: Hepatocellular carcinoma (HCC) is a significant health concern in China, and it has been leading cause of cancer-related deaths. Immunotherapy combined with anti-vascular growth therapy the first recommended therapy. In this study, we evaluated safety efficacy SCT-I10A (an anti-programmed death 1 [PD-1] monoclonal antibody) SCT510 (a bevacizumab biosimilar) compared to sorafenib as first-line treatment for advanced HCC. Methods: open-label, multicenter, phase 3 trial...
Coronaviruses such as severe acute respiratory syndrome (SARS) coronavirus (SCoV) and mouse hepatitis virus A59 (MHV-A59) have evolved strategies to disable the innate immune system for productive replication spread of infection. We previously shown that papain-like protease domain 2 (PLP2), a catalytic nonstructural protein 3 (nsp3) MHV-A59, encodes deubiquitinase (DUB) inactivates IFN regulatory factor (IRF3) thereby type I interferon (IFN) response.Here we provide further evidence PLP2...
We describe the role of PSF protein and VL30–1 RNA, a mouse retroelement noncoding in reversible regulation proto-oncogene transcription, cell proliferation, tumorigenesis mice. The experiments involved increasing expression or RNA NIH/3T3 fibroblast cells B16F10 melanoma by transfecting respective coding genes under control strong promoter decreasing shRNA construct that causes degradation mRNA RNA. results are as follows: ( i ) binds to Rab23 , repressing releases from activating...
The epithelial-to-mesenchymal transition is an important mechanism in cancer metastasis. Although transcription factors including SNAIL, SLUG, and TWIST1 regulate the transition, other unknown could also be involved. Identification of full complement essential for a more complete understanding gene regulation this process. Chromatin immunoprecipitation-sequencing (ChIP-Seq) technologies have been used to detect genome-wide binding factors; here, we developed systematic approach integrate...
The Mediator complex functions as a control center, orchestrating diverse signaling, gene activities, and biological processes. However, how subunits determine distinct cell fates remains to be fully elucidated. Here, we show that MED23 controls the fate preference directs differentiation into smooth muscle cells (SMCs) or adipocytes. Med23 deficiency facilitates SMC but represses adipocyte from multipotent mesenchymal stem cells. Gene profiling revealed presence absence of oppositely...