A5061353827- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Viral Infectious Diseases and Gene Expression in Insects
- Isotope Analysis in Ecology
- Vector-Borne Animal Diseases
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Physiological and biochemical adaptations
- Magnetic and Electromagnetic Effects
- Insect Utilization and Effects
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Genomics and Phylogenetic Studies
- Tardigrade Biology and Ecology
- Silkworms and Sericulture Research
- Spaceflight effects on biology
- Biocrusts and Microbial Ecology
- Aquatic Invertebrate Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Plant Virus Research Studies
- Fish Ecology and Management Studies
- Insect and Arachnid Ecology and Behavior
- Photosynthetic Processes and Mechanisms
National Agriculture and Food Research Organization
2019-2024
Institute for Sustainable Plant Protection
2023
Institute of Agrobiological Sciences
2002-2022
Kyushu Okinawa Agricultural Research Center
2019-2021
Pv11, a cell line derived from the anhydrobiotic insect, Polypedilum vanderplanki, was preserved in dry form (only 6% residual moisture) at room temperature for up to 251 days and restarted proliferating after rehydration. A previous study already reported survival of Pv11 cells desiccation, but without subsequent proliferation. Here, protocol improved increase achieve proliferation storage. The method basically included preincubation, desiccation rehydration processes each step...
Abstract The smaller tea tortrix, Adoxophyes honmai , has developed strong resistance to tebufenozide, a diacylhydrazine-type (DAH) insecticide. Here, we investigated its mechanism by identifying genes responsible for the tebufenozide using various next generation sequencing techniques. First, double-digest restriction site-associated DNA (ddRAD-seq) identified two candidate loci. Then, synteny analyses A. draft genome sequences revealed that one locus contained ecdysone receptor gene ( EcR...
Abstract The study of adipocyte differentiation and lipid accumulation in insects has been limited by the lack a system suitable for analysis molecular mechanisms. Here, we describe establishment model BmN4 cells, which are derived from silkworm ovary. In dexamethasone treatment induced lipid, suppressed cellular proliferation, caused cells to form aggregates. We isolated Bombyx mori fatty acid binding protein 1 gene ( BmFABP1 ), is homologue mouse Fabp4 aP2 marker mammals. expression was...
Abstract BACKGROUND The brown planthopper, Nilaparvata lugens (Stål), is one of the most notorious pests rice throughout Asia. planthopper has developed high resistance to imidacloprid, a member neonicotinoid insecticides. Several genes and mutations conferring imidacloprid in N. , especially eastern southeastern Asia populations, have been reported. Thus, key mechanisms need be examined. RESULTS RNA‐seq analyses revealed that only cytochrome P450 monooxygenase gene, CYP6ER1 was commonly...
Cultured cells are a very powerful tool for investigating biological events in vitro ; therefore, cell lines have been established not only model insect species, but also non-model species. However, there few reports on the establishment of stable and development systems to introduce genes into cultured honey bee ( Apis mellifera ). We describe simple ex vivo culture system bee. Hemocyte obtained from third fourth instar larvae were commercial Grace’s medium or MGM-450 more than two weeks...
Abstract Spodoptera frugiperda , an invasive pest insect that targets maize and other crops, first arrived in Japan the summer of 2019. This species occurs year‐round East Asian subtropical regions such as southern mainland China island Taiwan, where mean air temperature coldest month is above 10°C. Adults are similarly found throughout year on southwestern islands Japan. Trap monitoring there showed continuous or intermittent S. catches three winter seasons since However, it was difficult...
Abstract The hydrogen stable isotope ratios (δ 2 H) in tissues of terrestrial insects are widely applied to estimate natal origins field populations. isotopes insect incorporate those environmental waters through the insects’ metabolic processes. Water sources and abiotic factors reflect changes plant physiology, as indicated by δ H values plants. However, influence physiology on assimilation derived from water feeding diets is still unknown. We experimentally examined maize leaf forewings...
The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is an economically important polyphagous pest, which has recently expanded its distribution from the Americas to over 70 countries. Here, we established efficient rearing method for S. under crowded conditions. First, clarified larval density at there no decrease in survival rate due cannibalism. larvae were maintained on artificial diet till pupation five conditions: 10, 30, 50, 70, and 100 individuals per container (5.4L,...