A5028679670- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Metal complexes synthesis and properties
- Sirtuins and Resveratrol in Medicine
- Wound Healing and Treatments
- Endodontics and Root Canal Treatments
- Nuts composition and effects
- Immune Cell Function and Interaction
- Synthesis and Characterization of Heterocyclic Compounds
- Ferrocene Chemistry and Applications
- Bone Metabolism and Diseases
- X-ray Diffraction in Crystallography
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Dermatoglyphics and Human Traits
- Laser Applications in Dentistry and Medicine
- Renal and related cancers
- Cell death mechanisms and regulation
- Nanocomposite Films for Food Packaging
- Genomics, phytochemicals, and oxidative stress
- Carbon and Quantum Dots Applications
- Dendrimers and Hyperbranched Polymers
- Pancreatic and Hepatic Oncology Research
- Wnt/β-catenin signaling in development and cancer
Manisa Celal Bayar University
2016-2024
Izmir Atatürk Eğitim ve Araştırma Hastanesi
2023
Near East University
2022
Technical University of Munich
2020
Context: Almond oil is used in traditional and complementary therapies for its numerous health benefits due to high unsaturated fatty acids content.Objectives: This study investigated the composition vitro anticancer activity of almond from Northern Cyprus compared with Turkey.Materials methods: was obtained by supercritical CO2 extraction analyzed GC-MS. Turkey provided Turkish pharmacies. Different concentrations oils were incubated 24 48 h Colo-320 Colo-741 cells. Cell growth cytotoxicity...
Resveratrol and quercetin are phytochemicals that found in a variety of plants. The aim this study was to investigate the effect resveratrol on epithelial-mesenchymal transition (EMT) CD133+ CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from PANC-1 by MiniMACS system. treated with different concentrations (5, 10, 25, 50, 100 µM) quercetin. Cell growth cytotoxicity evaluated MTT assay. Anticancer anti-metastatic properties determined immunocytochemistry using...
Background: Quercetin is a flavonol from the flavonoid group of polyphenols, which positively affects human health due to its anti-cancer, anti-inflammatory, anti-microbial and cardioprotective effects. The effects phenolic compounds, including quercetin, on programmed cell death cellular senescence, have been subject research in recent years. Objective: In this study, we aimed investigate quercetin viability, apoptosis senescence primary (Colo-320) metastatic (Colo-741) colon adenocarcinoma...
Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients.The aim this study was to investigate the use platelet rich-plasma (PRP) and/or keratinocytelike cells (KLCs) in thermal wound rat model evaluate EGF, FGF-2, TGF-β1, COL1α2, MCP-1 VEGF-α as healing markers at gene expression level.Methods: In study, we used adipose tissue source mesenchymal stem (MSCs) differentiated MSCs into KLCs.KLCs were characterized transferred areas on dorsum...
Due to side effects and toxicity associated with platinum-derived metal-based drugs, extensive research has been conducted on ruthenium (Ru) complexes. We aim synthesize a highly oil soluble Ru(II)-p-cymene complex (Ru1) an aliphatic chain group, bimetallic (Ru2) N,S,S triple-coordination Ir(III)-pentamethylcyclopentadienyl (Ir1) S,S double-coordination. Subsequently, we investigate the of these complexes Vero HepG2 cell lines, focusing death mechanisms. Characterization is performed through...
Boswellia sacra Flueck essential oil is obtained from frankincense trees. The aim of this study was to investigate the in vitro anti-cancer activity (BS) CD133+ and CD133- Colo-320 cells. Essential analyzed by GC-MS. cells, cancer stem were MiniMACS system. Cytotoxicity evaluated with MTT assay. Anti-cancer BS investigated immunocytochemistry. 41 compounds identified oil. 1:2 dilution found be active against all cells for 24 h incubation. β-catenin immunoreactivity significantly decreased...
Opuntia ficus indica L. fruit (cactus pear) seed oil is used in traditional and complementary therapies for its numerous health benefits. The aim of this study was to analyse the fatty acid content apoptotic induction effects spiny thornless (CPS) oils. Spiny oils obtained by supercritical CO2 extraction method analyzed GC-MS. Different concentrations almond were incubated 24 h 48 with Colo-320 Colo-741 cells. Cell growth cytotoxicity measured MTT assays. TUNEL assay detect DNA fragmentation...
The aim of this study is to investigate the effects miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem (AD-MSCs).NK-like were AD-MSCs and activated by transfection. Transfected/non-transfected characterized immunohistochemical RT-PCR analyzes. Apoptotic efficiency transfected/non-transfected pancreatic cancer PANC1 determined TUNEL RT-PCR.In miR150-transfected cells, increased expression NK cell-specific genes such as GKMB, KIR2DL2, CD16, CD56,...
Several studies have suggested an oncogenic role for the neural Wiskott-Aldrich syndrome protein (N-WASP, encoded by Wasl gene), but thus far, little is known about its function in pancreatic ductal adenocarcinoma (PDAC). In this study, we performed silico analysis of WASL expression PDAC patients and found a correlation between low prolonged survival. To clarify carcinogenesis, used 2 Kras-based mouse models with pancreas-specific deletion. line human data, both had increased survival...
This study aimed to investigate the cytotoxicity and apoptotic activity of different endodontic sealers: Sealapex, Apexit Plus, AH MTA-Fillapex TotalFill BC Sealer in culture human periodontal ligament fibroblast (HPDLF) cells. The sealers were mixed, set for 24 h, then covered with medium obtain extracts, which diluted 1:0, 1:1, 1:2, 1:4, 1:8. Simultaneously, HPDLF cells (1 × 10
Among chemotherapeutic agents, cisplatin and the other platinum-based drugs have occupied for 35 years an enviable position. The limitations of drugs, dose dependent side effects development drug resistance mechanisms, boosted research finding metal-based drugs. metals, ruthenium is probably one showing greatest promises. Ruthenium (Ru) appears to be less toxic than platinum several biological studies indicated that complexes possess diverse modes action. redox chemistry rich compatible with...
Mesenchymal stem cells are self-renewing cells. The human foreskin has potential to be used as a source of aim the study was obtain spheroid formation (hnFSSCs) isolated from newborn tissue. In addition, apoptotic and proliferative effects traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated.After routine circumcision procedure cultured in suitable medium. plant leaves extracted with ethanol their composition analyzed by liquid chromatography...
Background: 2(3H)-Benzoxazolone derivatives are preferential structural blocks in pharmacological probe designing with the possibility of modifications at various positions on core structure. Benzoxazolones showed biological activities such as analgesics, anti-inflammatory and anti-cancer. Objective: In present work, we have prepared new Mannich bases 2(3H)-benzoxazolone evaluated their cytotoxicities proapoptotic properties MCF-7 breast cancer cell line. Methods: The structures these...
Carbon nanodots have drawn a great deal of attention due to their green and expedient opportunities in biological chemical sciences. Their high fluorescence capabilities low toxicity for living cells tissues make them excellent imaging agents. In addition, they fluorimetric response against inorganic organic species. Boron-doped carbon (B-CDs) with yield were produced from phenylboronic acid glutamine as boron sources, respectively, by hydrothermal method. First, the effects temperature on...
Thiosemicarbazide derivatives have been the focus of scientists owing to their broad biological activities such as anticancer, antimicrobial, and anti-inflammatory. Herein, we designed synthesized a new thiosemicarbazide derivative (TS-1) evaluated its antiproliferative potential against human hepatocellular carcinoma cell line (HEPG2) umbilical vein endothelial (ECV-304). Also, it was aimed investigate necroptotic apoptotic death effects TS-1 in HEPG2 cells, these were supported by...
Over the past years, inflammatory bowel disease (IBD) treatment has become more targeted, anticipating use of immune-modifying therapies at an earlier stage. During process prevention and management viral infections hold significant importance. The protective role favipiravir on enterocytes which are affected by inflammation is still unknown. We aim to analyze effects after condition. conducted a 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay assess cytotoxicity intestinal epithelioid cells...
BACKGROUND/AIMS: Coronavirus disease (COVID-19) is caused by a virus and exhibits various symptoms such as cough, fever, chills.Flavonoids have potential inhibitory effect on coronaviruses.In this study, we determined the effects of hesperidin enterocyte cells (IEC) after tumor necrosis factor (TNF)-α interferon (IFN)-γ induction which mimics severe acute respiratory therapy-coronavirus-2 (SARS-CoV-2) infection. MATERIALS AND METHODS:The IEC-6 were treated with 50 ng/mL TNF-α 100 IFN-ɣ for...
Trophoblasts are placenta-specific epithelial cells which play an important role in embryo implantation. Trophoblast stem (TSCs) precursor of placenta and can be derived before or after During TSCs culture, several molecules small vesicles such as exosome secreted. Exosomes, intercellular communication, signal transduction cell property regulation via carrying genetic material. The aim the study was to investigate released factors on human choriocarcinoma line (JAR). were obtained from E14...
Abstract Objective : Quercetin is a phytochemical that regarded as potential anticancer agent in colon cancer prevention. Exosomes are nanovesicles secreted by normal or cells, which transport miRNAs and proteins participate intercellular communication cargo. Cytotoxicity, exosomal miRNA secretion, distribution of Dicer, Ago2, CD9, CD63 eIF2α quercetin applied Colo 320 741 cell lines were aimed. Materials Methods The cytotoxicity test we used to evaluate viability MTT assay. both analyzed...