A5090354306- Molecular Biology Techniques and Applications
- Genetic Associations and Epidemiology
- Single-cell and spatial transcriptomics
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- RNA modifications and cancer
- Gene expression and cancer classification
- Traumatic Brain Injury and Neurovascular Disturbances
- Tryptophan and brain disorders
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Metabolism and Genetic Disorders
- Coronary Interventions and Diagnostics
- Peripheral Artery Disease Management
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Radiomics and Machine Learning in Medical Imaging
- Anesthesia and Neurotoxicity Research
- AI in cancer detection
- Brain Tumor Detection and Classification
- Genetic Mapping and Diversity in Plants and Animals
- Neuroscience of respiration and sleep
- Geological Modeling and Analysis
- Internet Traffic Analysis and Secure E-voting
- Heme Oxygenase-1 and Carbon Monoxide
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2025
Fu Wai Hospital
2017-2025
Bio-Medical Science (South Korea)
2024
Anhui University
2022
Pfizer (United States)
2015-2021
Zhejiang University of Science and Technology
2021
Harvard University
2013-2016
Center for Excellence in Molecular Cell Science
2016
University of Science and Technology of China
2016
Health First
2016
Abstract Scalable, integrative methods to understand mechanisms that link genetic variants with phenotypes are needed. Here we derive a mathematical expression compute PrediXcan (a gene mapping approach) results using summary data (S-PrediXcan) and show its accuracy general robustness misspecified reference sets. We apply this framework 44 GTEx tissues 100+ from GWAS meta-analysis studies, creating growing public catalog of associations seeks capture the effects variation on human...
Cell type composition, estimated from bulk tissue, maps the cellular specificity of genetic variants.
Developing axons can locally synthesize proteins, with roles in axon growth, guidance, and regeneration, but the mechanisms that regulate axonal mRNA translation are not well understood. MicroRNAs (miRNAs) important regulators of have still been little characterized developing axons. Here we study mouse dorsal root ganglion (DRG) show their extension is impaired by conditional deficiency miRNA-processing enzyme Dicer vitro vivo . A screen for localization identifies a specific set miRNAs...
While RNA-sequencing (RNA-seq) is becoming a powerful technology in transcriptome profiling, one significant shortcoming of the first-generation RNA-seq protocol that it does not retain strand specificity origin for each transcript. Without information difficult and sometimes impossible to accurately quantify gene expression levels genes with overlapping genomic loci are transcribed from opposite strands. It has recently become possible by modifying protocol, known as strand-specific or...
Acute neurological insults caused by infection, systemic inflammation, ischemia, or traumatic injury are often associated with breakdown of the blood-brain barrier (BBB) followed infiltration peripheral immune cells, cytotoxic proteins, and water. BBB extravasation these components into brain parenchyma result in oxidative stress, edema, excitotoxicity, neurodegeneration. These downstream consequences dysfunction can drive pathophysiological processes play a substantial role morbidity...
The first-in-class treatment PF-06480605 targets the tumor necrosis factor-like ligand 1A (TL1A) molecule in humans. Results from phase 2a TUSCANY trial highlighted safety and efficacy of ulcerative colitis. Preclinical vitro models have identified a role for TL1A both innate adaptive immune responses, but mechanisms underlying anti-TL1A inflammatory bowel disease (IBD) are not known.Here, we provide analysis tissue transcriptomic, peripheral blood proteomic, fecal metagenomic data recently...
RNA sequencing (RNA-seq), a next-generation technique for transcriptome profiling, is being increasingly used, in part driven by the decreasing cost of sequencing. Nevertheless, analysis massive amounts data generated large-scale RNA-seq remains challenge. Multiple algorithms pertinent to basic analyses have been developed, and there an increasing need automate use these tools so as obtain results efficient user friendly manner. Increased automation improved visualization will help make...
As genome-wide association studies (GWAS) have grown in size, the number of genetic variants that been associated per disease has correspondingly increased. Despite this increase single-nucleotide polymorphisms (SNPs) identified disease, their biological interpretation many cases remained elusive. To address this, we combined GWAS results with orthogonal sources evidence, namely current knowledge molecular pathways; real-world clinical data from six million patients; RNA expression across...
Abstract The Genotype-Tissue Expression (GTEx) project has identified expression and splicing quantitative trait loci ( cis -QTLs) for the majority of genes across a wide range human tissues. However, interpretation these QTLs been limited by heterogeneous cellular composition GTEx tissue samples. Here, we map interactions between computational estimates cell type abundance genotype to identify interaction seven types show that eQTLs provide finer resolution specificity than bulk -eQTLs....
Abstract Differences in the expression of genes and their splice isoforms across human tissues are fundamental factors to consider for therapeutic target evaluation. To this end, we conducted a transcriptome-wide survey tissue-specific gene splicing events unprecedented collection 8527 high-quality RNA-seq samples from GTEx project, covering 36 peripheral 13 brain subregions. We derived weighted tissue-specificity scoring scheme accounting similarity related inherent variability individual...
RNA-based regulatory mechanisms play important roles in the development and plasticity of neural circuits neurological disease. Developing axons provide a model well suited to study regulation, contain specific subsets mRNAs that are locally translated have axon pathfinding. However, RNA-binding proteins involved pathfinding, their corresponding mRNA targets, still largely unknown. Here we find protein IMP2 (Igf2bp2) is strikingly enriched developing tracts, including spinal commissural...
Cell migration is orchestrated by dynamic interactions of microtubules with the plasma membrane cortex. How these facilitate processes still being actively investigated. TIP150 a newly characterized microtubule plus end tracking protein essential for mitosis and entosis (Ward, T., Wang, M., Liu, X., Z., Xia, P., Chu, Y., L., Jiang, K., Yu, H., Yan, J., Hill, D. Huang, Zhu, Yao, X. (2013) Regulation interaction between two microtubule-binding proteins, EB1 TIP150, mitotic p300/CBP-associated...
The advent of Next Generation Sequencing has allowed transcriptomes to be profiled with unprecedented accuracy, but the high costs full-length mRNA sequencing have posed a limit on accessibility and scalability technology. To address this, we developed 3'Pool-seq: simple, cost-effective, scalable RNA-seq method that focuses 3'-end mRNA. We drew from aspects SMART-seq, Drop-seq, TruSeq implement an easy workflow, optimized parameters such as input RNA concentrations, tagmentation conditions,...
Summary GWAS have identified 108 loci that confer risk for schizophrenia, but mechanisms individual are largely unknown. Using developmental, genetic, and illness-based RNA sequencing expression analysis, we characterized the human brain transcriptome around these found enrichment developmentally regulated genes with novel examples of shifting isoform usage across pre- post-natal life. We widespread quantitative trait (eQTLs), including many transcript specificity previously unannotated...
Brain injury is a major complication resulted from cardiopulmonary bypass (CPB). Dexmedetomidine (DEX) has potential brain-protective effects; however, the mechanism unclear. The aim of this study to investigate effect DEX on brain in CPB rats and its mechanism. levels interleukin-6 (IL-6), interleukin-10 (IL-10), S100β, neuron-specific enolase (NSE) were measured by enzyme-linked immunosorbent assay. hippocampus CA1 region was observed hematoxylin-eosin staining. Western blot quantitative...
Analyzing X-ray images is a critical method for diagnosing breast cancer. The prompt and accurate detection of masses from extensive image data vital in reducing cancer incidence mortality rates. While deep learning-based approaches show promise effectively detecting cancer, they still face challenges. Specifically, these struggle to extract features small objects lack multi-scale feature fusion. Addressing issues crucial more robust detection. In this paper, we propose the Multi-scale...