A5028418094- Fatty Acid Research and Health
- Eicosanoids and Hypertension Pharmacology
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Antioxidant Activity and Oxidative Stress
- Hormonal Regulation and Hypertension
- Inflammatory mediators and NSAID effects
- Nitric Oxide and Endothelin Effects
- Renal function and acid-base balance
- Alcohol Consumption and Health Effects
- Acute Kidney Injury Research
- Diet and metabolism studies
- Asthma and respiratory diseases
- Gestational Diabetes Research and Management
- Reproductive System and Pregnancy
- Free Radicals and Antioxidants
- Slime Mold and Myxomycetes Research
- Pharmacogenetics and Drug Metabolism
- Hemodynamic Monitoring and Therapy
- Diatoms and Algae Research
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
- Renin-Angiotensin System Studies
- Cholesterol and Lipid Metabolism
- Pregnancy and Medication Impact
The University of Western Australia
2014-2024
Royal Perth Hospital
2014-2024
Imperial College London
2023
Deakin University
2006
Harry Perkins Institute of Medical Research
2004-2005
The Kids Research Institute Australia
2003
The Heart Research Institute
2002
King Edward Memorial Hospital
2001
National Heart Foundation of Australia
2001
National Health and Medical Research Council
2001
Abstract BACKGROUND Resolvins and protectins are families of local lipid mediators generated from the n-3 fatty acids eicosapentaenoic acid (EPA) docosahexaenoic (DHA) during self-limited resolution inflammation. We aimed to develop a liquid chromatography–tandem mass spectrometry (LC-MS/MS) assay measure these in human blood following supplementation determine whether collection method affects their measured concentration. METHODS Blood samples 20 healthy volunteers enrolled an trial were...
Summary Background and objectives The epidemiological association between higher dietary n‐3 polyunsaturated fatty acids (PUFA) lower prevalence of asthma, has led to interest in the role early modification allergic disease prevention. In this study we examined effects maternal (PUFA)‐rich fish oil supplementation on cord blood (CB) IgE cytokine levels neonates at risk developing disease. Methods a randomized double‐blind, placebo‐controlled trial, 83 atopic pregnant women received either...
Omega-3 and n-6 fatty acids are biosynthetic precursors to lipid mediators with antinociceptive pronociceptive properties. We conducted a randomized, single-blinded, parallel-group clinical trial assess biochemical effects of targeted alteration in dietary n-3 for treatment chronic headaches. After 4-week preintervention phase, ambulatory patients daily headache undergoing usual care were randomized 1 2 intensive, food-based 12-week interventions: high plus low (H3-L6) intervention, or (L6)...
Arachidonic acid is a major fatty that can be metabolized by the cytochrome P450 enzyme to number of bioactive eicosanoids. A metabolite this oxidation 20-hydroxyeicosatetraenoic acid, which acts as potent vasoconstrictor. However, in kidney, its vasoconstrictor actions offset natriuretic properties. guanine-to-adenine polymorphism CYP4F2 gene was associated with reduction production vitro. thymidine-to-cytosine CYP4A11 reduced catalytic activity >50% vitro and hypertension. The aim...
Abstract Background Inadequate inflammation resolution may contribute to persistent low-grade that accompanies many chronic conditions. Resolution of is an active process driven by Specialized Pro-resolving Mediators (SPM) derive from long chain n-3 and n-6 fatty acids. This study examined plasma SPM in relation sex differences, lifestyle a broad range cardiovascular disease (CVD) risk factors 978, 27-year olds the Australian Raine Study. Methods Plasma pathway intermediates (18-HEPE,...
A positive relationship between alcohol consumption and blood pressure (BP) is well-established but the relative effect of specific alcoholic beverages controversial. This study aimed to determine whether red wine may improve vascular function have less an impact on because its high content antioxidant vasodilator polyphenolic compounds. Healthy normotensive men entered a 4-period crossover comparing in random order 4 weeks control-abstinence with similar periods daily (375 mL; 39 grams...
1. This study was designed to seek evidence for excessive lipid peroxidation in pre-eclamptic women using 8-iso-prostane as a novel bioactive marker of vivo. Plasma free, total and urinary were measured 20 with proteinuric pre-eclampsia, compared 18 age- gestation-matched pregnant control subjects, before delivery at 6 weeks postpartum. 2. free significantly elevated the subjects delivery, fell levels post-partum. Conversely, normal pregnancy rose post-partum 3. Total plasma not during...
Consumption of fish or oils rich in the n -3 long chain PUFA EPA and DHA may improve multiple risk factors for CVD. The objective this study was to determine whether regular consumption foods enriched with long-chain can status (erythrocytes) cardiovascular health. Overweight volunteers high levels triacylglycerols (TG; >1·6 mmol/l) were enrolled a 6-month dietary intervention trial conducted Adelaide ( 47) Perth 39), randomised consume control -3-enriched achieve an + intake 1 g/d. Test...
Enhanced oxidative stress is implicated in the development of atherosclerosis humans and animal models. F(2)-isoprostanes are formed vivo via free radical peroxidation arachidonic acid, their quantification has allowed assessment vivo. associate with lipids, although distribution human plasma lipoproteins unknown. Our aim was to determine levels isolated from by ultracentrifugation fast protein liquid chromatography (FPLC). were significantly higher HDL compared LDL or VLDL after isolation...
The aim of this study was to determine whether supplementation with fish oil-derived n-3 polyunsaturated fatty acids (n-3 PUFA) during pregnancy modifies placental PUFA composition, the accumulation specialised pro-resolving lipid mediators (SPMs, specifically resolvins (Rv), protectins (PD) and upstream precursors) inflammatory gene expression. Placentas were collected from women (n=51) enrolled in a randomised, placebo controlled trial 20-week gestation. Lipids extracted for acid analysis...
Resolution of inflammation is an active process involving specialized proresolving mediators (SPM) formed from the n-3 fatty acids. This study examined effect acid supplementation and aspirin on plasma SPMs in healthy humans. Healthy volunteers (n = 21) were supplemented with acids (2.4g/day) for 7 days random assignment to take (300 mg/day) or placebo day 5 7. Blood was collected at baseline (day 0), 5, Plasma 18R/S-HEPE, E-series resolvins, 17R/S-HDHA, D-series 14R/S-HDHA, MaR-1 measured...
The anti-inflammatory properties of n-3 polyunsaturated fatty acids (n-3 PUFA) have suggested a potential role these nutrients in dietary modification for prevention allergic disease early life. As oxidative stress is known to modify antigen presenting cell (APC) signalling and resulting immune responses, we examined the effects maternal PUFA supplementation pregnancy on markers APC function neonates at high risk allergy. Eighty-three pregnant atopic women were randomised receive 4 g daily...
CYP450AAM [arachidonic acid metabolites of the CYP450 (cytochrome P450) enzyme system] have a range biological functions. are involved in pathogenesis hypertension, renal function and vascular function, yet their role stroke has not been clarified. We aimed at determining levels circulating patients with acute ischaemic (<96 h) compared healthy age- gender-matched controls. This was retrospective case-controlled study 44 matched A subset available for follow-up. Acute had elevated plasma...