A5062489018- Renal and related cancers
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Prenatal Screening and Diagnostics
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Sex work and related issues
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Urologic and reproductive health conditions
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Hybrid Renewable Energy Systems
- Testicular diseases and treatments
- Sexual Differentiation and Disorders
University of Science and Technology of China
2021-2025
Hefei National Center for Physical Sciences at Nanoscale
2024
First Affiliated Hospital of Henan University of Science and Technology
2022
In mammals, the production of mature oocytes necessitates rigorous regulation discontinuous meiotic cell-cycle progression at both transcriptional and post-transcriptional levels. However, factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize function N-acetyltransferase 10 (Nat10), a writer for N4-acetylcytidine (ac4C) on RNA molecules, in mouse oocyte development. We provide genetic evidence that Nat10 is essential prophase I progression,...
CRISPR/Cas9 genome-editing tools have tremendously boosted our capability of manipulating the eukaryotic genomes in biomedical research and innovative biotechnologies. However, current approaches that allow precise integration gene-sized large DNA fragments generally suffer from low efficiency high cost. Herein, we developed a versatile efficient approach, termed LOCK (Long dsDNA with 3'-Overhangs mediated CRISPR Knock-in), by utilizing specially designed 3'-overhang double-stranded (odsDNA)...
Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. However, genetic factors underlying this human disease remain largely unknown. The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation chromosomes throughout cell cycles. Once kinetochores are correctly attached to bipolar spindles and SAC satisfied, MAD2L1BP, best known as p31comet, binds mitosis deficient 2 (MAD2)...
Arginine methylation, catalyzed by the protein arginine methyltransferases (PRMTs), is a common post-translational modification (PTM) that engaged in plethora of biological events. However, little known about how methylarginine-directed signaling functions germline development. In this study, we discover Prmt1 predominantly distributed nuclei spermatogonia but weakly spermatocytes throughout mouse spermatogenesis. By exploiting combination three Cre-mediated knockout lines, unravel essential...
Abstract Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. However, genetic factors underlying this human disease remain largely unknown. The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation chromosomes throughout cell cycles. Once kinetochores are correctly attached to bipolar spindles and SAC satisfied, MAD2L1BP, best known as p31 comet , binds MAD2 recruits...
Abstract In mammals, the production of mature oocytes necessitates rigorous regulation discontinuous meiotic cell-cycle progression at both transcriptional and post- levels; however, factors underlying this sophisticated but explicit process during oocyte development remain largely unclear. Here we characterized function N-acetyltransferase 10 (Nat10), which was previously recognized as a “writer” for N4-acetylcytidine (ac4C) deposited on RNA molecules. We generated two germline-specific...