The aim of this study was to assess the relationship between extracorporeal blood flow (EBF) and left ventricular (LV) performance during venoarterial membrane oxygenation (VA ECMO) therapy.Five swine (body weight 45 kg) underwent VA ECMO implantation under general anesthesia artificial ventilation. Subsequently, acute cardiogenic shock with signs tissue hypoxia induced. Hemodynamic cardiac parameters were then measured at different levels EBF (ranging from 1 5 L/min) using arterial venous...

Extracorporeal membrane oxygenation (ECMO) is increasingly used in cardiac arrest (CA). Adequacy of carotid and coronary blood flows (CaBF, CoBF) perfusion pressure (CoPP) ECMO treated CA not well established. This study compares femoro-femoral (FF) to femoro-subclavian (FS) intraaortic balloon counterpulsation (IABP) contribution based on CaBF, CoBF, CoPP, myocardial brain experimental managed by ECMO.In 11 female pigs (50.3 ± 3.4 kg), was randomly FF versus FS IABP. Animals under general...

Several percutaneous circulatory support systems have been recently introduced into clinical practice for the treatment of cardiogenic shock or refractory nontolerated ventricular tachycardia, in high-risk catheter interventions and, occasionally, cardiopulmonary resuscitation. To date, however, a direct comparison available has not performed.Adult female pigs (weight 50-60 kg) were used throughout experiment. Under deep anesthesia and mechanical ventilation, 3 compared: (1) right...

BackgroundAdrenaline (ADR) is a cornerstone of advanced life support (ALS) in cardiac arrest (CA), although its neurologically favourable survival outcomes remain unclear. ADR increases coronary perfusion pressure (CPP), with levels >15 mmHg associated successful defibrillation. This study aimed to elucidate the relationship between ADR, myocardial microvascular blood flow, and resuscitation using porcine CA model simulating refractory ventricular fibrillation (VF).MethodsThis involved 24...

The effects of a large arteriovenous fistula (AVF) on pulmonary perfusion remains to be elucidated. We aimed study, for the first time, real-time acute AVF regional distribution in novel porcine model. Ten healthy swine under general anesthesia were studied. was created by connection femoral artery and vein using high-diameter cannulas. closed after 30 min stabilization values recorded. then opened, new data collected reaching stable state. Continuous hemodynamic monitoring performed...

Abstract Background Bronchoscopic lung volume reduction (BLVR) with one-way endobronchial valves (EBV) has better outcomes when the target lobe poor collateral ventilation, resulting in complete atelectasis. High-inspired oxygen fraction (F I O 2 ) promotes atelectasis through faster gas absorption after airway occlusion, but its application during BLVR EBV been poorly understood. We aimed to investigate real-time effects of F on regional volumes and ventilation/perfusion by electrical...

The aim of the study was to detect changes both QT dispersion and T-loop morphology resulting from changed spatial position heart during pregnancy. Electrocardiographic vectorcardiographic recordings were obtained 37 healthy women 19-36 years old in 36th 40th week physiological pregnancy 2 6 days after delivery. same 18 age. average (+/- S.D.) normal subjects significantly lower (34 +/- 12 ms) than those late (73 (P < 0.001). amplitude (Ta) 0.001) smaller (532 98 microV) width (Tw) wider...

The aim of our study was to compare the responses heart rate variability (HRV) with two different types hormonal substitution therapy (HT) in post-menopausal women (cross-sectional study) and reveal an effect HT shortly after beginning its administration (follow-up study). To elucidate influence menopause effects protocols a on autonomic control rate, we evaluated 5 groups: premenopausal (n=140), postmenopausal without (n=360), conjugated estrogen only (n=168), continuous combined...

Mild therapeutic hypothermia (HT) has been implemented in the management of post cardiac arrest (CA) syndrome after publication clinical trials comparing HT with common practice (ie, usually hyperthermia). Current evidence on comparison between and controlled normothermia (NT) CA survivors, however, remains insufficient. Eight female swine (sus scrofa domestica; body weight 45 kg) were randomly assigned to receive either mild or NT, four animals per group. Veno-arterial extracorporeal...

Introduction Veno-arterial extracorporeal life support (ECLS) is increasingly being used to treat rapidly progressing or severe cardiogenic shock. However, it has been repeatedly shown that increased afterload associated with ECLS significantly diminishes left ventricular (LV) performance. The objective of the present study was compare LV function and coronary flow during standard continuous-flow electrocardiogram (ECG)-synchronized pulsatile in a porcine model Methods Sixteen female swine...

The aim of this study was to quantify and understand the unloading effect percutaneous balloon atrial septostomy (BAS) in acute cardiogenic shock (CS) treated with venoarterial (VA) extracorporeal membranous oxygenation (ECMO). In CS VA ECMO, increased left ventricular (LV) afterload is observed that commonly interferes myocardial recovery or even promotes further LV deterioration. Several techniques for exist, but optimal strategy actual extent such procedures have not been fully disclosed....

Current research highlights the role of microcirculatory disorders in post-cardiac arrest patients. Affected microcirculation shows not only dissociation from systemic hemodynamics but also strong connection to outcome these However, few studies evaluated directly during cardiac (CA) and cardiopulmonary resuscitation (CPR). The aim our experimental study a porcine model was describe sublingual changes CA CPR using recent videomicroscopic technology provide comparison parameters global...

Abstract Background Among the challenges for personalizing management of mechanically ventilated patients with coronavirus disease (COVID-19)-associated acute respiratory distress syndrome (ARDS) are effects different positive end-expiratory pressure (PEEP) levels and body positions in regional lung mechanics. Right-left aeration asymmetry poorly recruitable lungs increased recruitability alternating position between supine prone have been reported. However, real-time changing PEEP on...

Our study presents a novel collagen wound dressing prepared from freshwater fish skin ( Cyprinus Carpio) type I. Half of the sponges were cross-linked with carbodiimide. The and non-cross-linked subsequently impregnated gentamicin lyophilized thus allowing for attainment appropriate content without removal thereof during cross-linking stage. structure was evaluated via micro-CT infrared spectrometry structural stability release properties in phosphate buffer solution. further tested rat...

The peripheral venoarterial extracorporeal life support (V-A ECLS) in cardiogenic shock (CS) may lead to LV overload. transaortic suction device (Impella, ABIOMED Inc., Danvers, MA) was compared the pulmonary artery (PA) drainage, for unloading efficacy during V-A ECLS a porcine model. A dedicated CS model included 12 swine (21 ± 1.8-week-old and weighing 54.3 4.6 kg) supported with randomized Impella or PA-related drainage. end-organ perfusion were evaluated through PA catheter...

Diabetes mellitus is a risk factor of cardiovascular diseases. ECG patients with diabetes type 1 (DM 1) shows tachycardia (block parasympathetic innervation) and abnormal repolarization (increased QT interval dispersion (QTd)) indicating ventricular sudden death in young people DM 1. The aim the present report was to measure 145 parameters heart electric field 22 (14 men, 8 women) without complications (mean age 32.8+/-11.4 years) controls (11 11 women, mean 30.1+/-3.4 years). duration...

Despite the urgent need for experimental research in field of acute heart failure and, particularly cardiogenic shock, currently there are only limited options large animal models enabling using devices applied to human subjects. The majority available either associated with an unacceptably high rate mortality or incapable developing sufficient severity failure. objective our was develop a novel model severe shock. Advanced left ventricular dysfunction induced by global myocardial hypoxia...

Aim . To assess the systemic and local immunological response to subcutaneous implants of a vascular graft covered with collagen extracted from European carp (freshwater fish) or bovine origin. Methods Pieces by pure (Bos taurus, BOV, n=14) (Cyprinus carpio, CYP, 5 mm in size were implanted subcutaneously dorsum Balb/cOla mice. A sham operation group 12 animals served as control. At 7 14 days after operation, one-half each was terminated blood for serum, spleen, implant surrounding tissue...

Low-volume lung injury encompasses local concentration of stresses in the vicinity collapsed regions heterogeneously ventilated lungs. We aimed to study effects on ventilation and perfusion distributions a sequential lateral positioning (30°) strategy using electrical impedance tomography imaging porcine experimental model early acute respiratory distress syndrome (ARDS). hypothesized that such strategy, including real-time individualization positive end-expiratory pressure (PEEP) whenever...

Aim . The aim of this study was to evaluate short-term patency the new prosthetic graft and its structural changes after explantation. Methods team developed a three-layer conduit composed scaffold made from polyester coated with collagen inner outer side an internal diameter 6 mm. implanted as bilateral bypass carotid artery in 7 sheep stenosis created selected animals. After period 161 days, explants were evaluated gross microscopic specimens. Results initial flow rate (median ± IQR)...

Relationship between regional tissue oxygenation (rSO2) and microcirculatory changes during cardiac arrest (CA) are still unclear. Therefore, we designed an experimental study to correlate rSO2, microcirculation systemic hemodynamic parameters in a porcine model of CA.Ventricular fibrillation was induced 24 female pigs (50±3kg) left for three minutes untreated followed by five mechanical CPR. Regional peripheral saturations were assessed near-infrared spectroscopy, sublingual Sidestream Dark...

The study presents a novel vancomycin-releasing collagen wound dressing derived from Cyprinus carpio type I cross-linked with carbodiimide which retarded the degradation rate and increased stability of sponge. Following lyophilization, dressings were subjected to gamma sterilization. structure was evaluated via scanning electron microscopy images, micro-computed tomography, infrared spectrometry. structural vancomycin release properties in phosphate buffered saline. Microbiological testing...

The geometric shape of the distal anastomosis in an infrainguinal bypass has influence on its durability. In this article, we compared three different angles with regard to hemodynamics. Three experimental models 25°, 45°, and 60° respectively were constructed according similarity theory assess flow anastomoses using particle image velocimetry computational fluid dynamics. toe, heel, floor that correspond locations worst affected by intimal hyperplasia, adverse blood wall shear stress...