A5027106880- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Fish Ecology and Management Studies
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Graphene and Nanomaterials Applications
- Carbon and Quantum Dots Applications
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Advanced Biosensing Techniques and Applications
- bioluminescence and chemiluminescence research
- RNA Research and Splicing
- Quantum Dots Synthesis And Properties
- Tissue Engineering and Regenerative Medicine
- Autophagy in Disease and Therapy
- S100 Proteins and Annexins
- DNA and Nucleic Acid Chemistry
Catholic Kwandong University
2014-2019
Government of the Republic of Korea
2014-2019
Gangneung Asan Hospital
2014-2019
International St. Mary's Hospital
2014-2018
CHA University
2009-2016
Auburn University
2000-2016
Korea University
2011
Seoul National University
2006-2009
Seoul Medical Center
2008
Korea National Institute of Health
2002-2006
The recent advances in molecular imaging techniques, using cancer-targeting nanoparticle probes, provide noninvasive tracking information on cancer cells living subjects. Here, we report a multimodal cancer-targeted system capable of concurrent fluorescence imaging, radionuclide and MRI vivo. <b>Methods:</b> A cobalt–ferrite surrounded by fluorescent rhodamine (designated MF) within silica shell matrix was synthesized with the AS1411 aptamer (MF-AS1411) that targets nucleolin (a cellular...
Glioma stem cells (GSC) possess tumor-initiating potential and are relatively resistant to conventional chemotherapy irradiation. Thus, they considered be major drivers for glioma initiation, progression, recurrence. However, the precise mechanism governing acquisition of their drug resistance remains elucidated. Our previous study has shown that inhibitor differentiation 4 (ID4) dedifferentiates Ink4a/Arf(-/-) mouse astrocytes human stem-like (induced GSCs or iGSCs). In this article, we...
MicroRNAs (miRNAs) function in mammalian cells via translational repression or messenger RNA (mRNA) cleavage of target genes by base-pairing with 3' untranslated regions (UTRs) mRNAs. Although miRNAs are involved cell differentiation organ development, posttranscritptional regulation miRNA is not well understood. Here, we developed a dual-luciferase reporter system for monitoring vivo endogenous transcription primary (pri-miRNA) and also the mature activity simultaneously.miR23P639/Fluc...
Aptamers conjugated with quantum dots (QDs) bind to target molecules on the cellular membrane of cancer cells. QDs a carboxyl terminal are three types tumor-targeting aptamer (see picture). Various cell simultaneously targeted QD-TTA1 (605nm, light green), QD-AS1411 (655nm, red), and QD-MUC-1 (705nm, violet). Detailed facts importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by...
An imaging system that can be used to evaluate the expression levels of microRNAs during neuronal development provide noninvasive information for investigating a variety biological phenomena related (miRNAs, miRs). Herein, novel platform monitor intracellular miR124a differentiation is reported using rhodamine-coated cobalt ferrite magnetic fluorescent (MF) nanoparticles linked quenching molecular containing an binding sequence (MF-miR124a beacon). During differentiation, in vitro...
We report smart nanoprobe, hyaluronic acid (HA)-based nanocontainers containing miR-34a beacons (bHNCs), for the intracellular recognition of levels in metastatic breast cancer cells, which is distinct from imaging biomarkers such cell membrane receptors as HER2. In this study, we demonstrate that a nanoscale vesicle couples targeting endocytic route, CD44, and molecular probe enables efficient detection specific miRNAs. Furthermore, bHNCs showed no cytotoxicity high stability due to...
NIRF imaging of carbon nanodots derived from tire soot was clearly visualized in glioma vitro and vivo.
Using the excellent emission properties of carbon nanodots, we have developed an aptamer-conjugated imaging probe for targeting cancers.
Abstract Introduction Mesenchymal stem cells (MSCs) have therapeutic potential for the repair of myocardial injury. The efficacy MSC therapy regeneration mainly depends on survival after transplantation into infarcted heart. In transplanted regions, reactive oxygen species (ROS) can cause cell death, and this process caspase activation autophagosome formation. Methods A Software TargetScan was utilized to search microRNAs (miRNAs) that target caspase-3 mRNA. Six candidate miRNAs including...
MicroRNAs (miRNAs) are small, noncoding RNA molecules that control expression of target genes. The abnormally expressed miRNAs function as oncogenes or tumor suppressors in human cancer. To evaluate the abundant gene regulation miR-221 papillary thyroid carcinoma (PTC), we performed microarray analysis and developed a <i>Gaussia</i> luciferase (Gluc) reporter system regulated by miR-221. <b>Methods:</b> Total RNAs were isolated from pre-miR-221–treated normal cells (HT-ori3)...
Abstract Semiconductor quantum dots (QDs), which have broad absorption with narrow emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and arginine–glycine–aspartic acid integrin α v β 3 ) in cancer cells is reported. Simultaneous of cellular distribution nucleolin enables easy monitoring separate targets normal healthy cells. These results suggest feasibility a concurrent...
Abstract The combination of nanotechnology with molecular imaging has great potential for the development diagnostics and therapeutics, multimodal enables versatile applications from cell tracking in animals to clinical applications. Herein, we report a nanoparticle system that is capable concurrent fluorescence, bioluminescence, bioluminescence resonance energy transfer (BRET), positron emission tomography (PET) magnetic (MR) vivo. A cobalt–ferrite surrounded by rhodamine (MF) was...
Nanomaterials have been widely evaluated for potential use as efficient delivery carriers cancer diagnosis and therapy. To translate these nanomaterials to the clinic, their safety needs be verified, particularly in terms of genotoxicity cytotoxicity. We investigated changes gene expression profiles influenced by silica-coated cobalt ferrite magnetic-fluorescence nanoparticles silica-free magnetic-core vivo vitro.(68)Ga-labeled produced synthesis...
Chemically modified nucleotides have been developed and applied into SELEX procedure to find a novel type of aptamers fit with targets interest. In this study, we directly performed chemical modification 5-(<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>N</mml:mi></mml:math>-benzylcarboxyamide)-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msup><mml:mn>2</mml:mn><mml:mo>′</mml:mo></mml:msup></mml:math>-deoxyuridine (called 5-BzdU) in the AS1411 aptamer,...
Abstract Competition for cellular iron (Fe) is a vital component of the interaction between host and pathogen. Most bacteria have an obligate requirement Fe to sustain infection, growth, survival in host. To obtain required many secrete chelators (siderophores). This study was undertaken test whether bacterial siderophore, deferoxamine (DFO), could trigger inflammatory signals human intestinal epithelial cells as single stimulus. Incubation HT-29 with DFO increased expression IL-8 mRNA, well...
Generally, the 3'-end of duplex microRNA (miR) precursor (pre-miR) is known to be stable in vivo and serve as a mature form miR. However, both (miR9) 5'-end (miR9*) brain-specific miR9 have been shown function biologically brain development. In this study, real-time PCR analysis vitro/in bioluminescent imaging demonstrated that upstream region primary miR9-1 (pri-miR9-1) can used monitor highly expressed pattern endogenous pri-miR9-1 during neurogenesis, Luciferase reporter gene image...
MicroRNAs (miRNAs, miRs) are recognized as regulators of gene expression related to cellular development and diseases. In this study, we developed a carbon nanodot (C-dot)-based miR124a molecular beacon (miR124a CMB). The C-dots were purified from candle soot (cC-dots) by thermal oxidation. double-stranded DNA oligonucleotide containing binding site black hole quencher 1 sensing oligo) was further conjugated with the cC-dots form CMB. P19 cells incubated CMB sense during neurogenesis....