A5074315138- Erythrocyte Function and Pathophysiology
- Connexins and lens biology
- Hemoglobinopathies and Related Disorders
- Heat shock proteins research
- Advanced Proteomics Techniques and Applications
- Reproductive tract infections research
- Traumatic Ocular and Foreign Body Injuries
- Mosquito-borne diseases and control
- Neonatal Health and Biochemistry
- Heme Oxygenase-1 and Carbon Monoxide
- Retinal Development and Disorders
- Malaria Research and Control
- Ophthalmology and Eye Disorders
- Neuroblastoma Research and Treatments
- Hemoglobin structure and function
- Protein Structure and Dynamics
- Cancer, Hypoxia, and Metabolism
- COVID-19 Clinical Research Studies
- Bacterial Identification and Susceptibility Testing
- Blood groups and transfusion
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Drug-Induced Ocular Toxicity
- Facial Trauma and Fracture Management
- Corneal surgery and disorders
University of Palermo
2025
University of Trento
2022
Associazione Istituzione Libera Università Nuorese
2022
ATS Sardegna (Italy)
2022
University of Sassari
1997-2010
Institute of Ophthalmology
2009-2010
University of Turin
2003-2007
Universidad de Sevilla
1999-2001
Delft University of Technology
2001
Università Cattolica del Sacro Cuore
1999
Oxidative events involving band 3 (Anion Exchanger 1) have been associated with RBC (red blood cell) removal through binding of NAbs (naturally occurring antibodies); however, the underlying mechanism has only partially characterized. In addition to inducing direct membrane protein oxidative modification, treatment specifically triggers phosphorylation tyrosine residues. The present study reports that diamide, a thiol group oxidant, induces disulfide cross-linking poorly glycosylated and...
<h3>AIMS</h3> To identify and determine antibiotic susceptibility of coagulase negative staphylococci (CoNS) isolated from patients with chronic blepharitis, purulent conjunctivitis, suppurative keratitis. <h3>METHODS</h3> A retrospective review all culture positive cases keratitis between July 1995 December 1996 was performed. Cases in which CoNS were the sole isolates analysed. Species identification performed by using a commercially available standardised biochemical test system....
To investigate a possible correlation between final visual acuity and the presence at baseline of various systemic local (orbital/ocular) signs in patients affected by indirect traumatic optic neuropathy.35 cases neuropathy were examined retrospectively 13 variables tested. Univariate analysis with "no recovery acuity" as primary outcome was performed. Relative risk (RR) 95% confidence intervals (CI) calculated. Fisher's exact test used for two to differences proportions.Four showed...
High counts of circulating microparticles, originated from the membrane abnormal erythrocytes, have been associated with increased thrombotic risk in hemolytic disorders. Our studies indicate that thalassemia intermedia patients number microparticles correlates capability thalassemic erythrocytes to release microparticles. The are characteristically loaded hemichromes formed by denatured α-chains. This finding was substantiated positive correlation observed between amount measured their and...
Abstract Neuroblastoma (NB) and Ewing’s sarcoma (ES) represent the most common extracranial solid tumors of childhood. Heat shock proteins (HSP) are elevated in cancer cells their over‐expression was correlated to drug‐resistance. In this work we identified HSP by a sensitive proteomic analysis NB ES cell lines, then, studied response doxorubicin. Some were constitutively more expressed than cells. Doxorubicin‐stimulated only Quercetin found inhibit expression depleting heat factor 1 (HSF1)...
Apolipoproteins are very heterogeneous protein family, implicated in plasma lipoprotein structural stabilization, lipid metabolism, inflammation, or immunity. Obtaining detailed information on apolipoprotein composition and structure may contribute to elucidating roles atherogenesis developing new therapeutic strategies for the treatment of lipoprotein-associated disorders. This study aimed at a comprehensive method characterizing component VLDL, LDL, HDL fractions from patients undergoing...
Abstract Phosphorylation of erythrocyte membrane proteins has been previously documented following infection and intracellular growth the malarial parasite, Plasmodium falciparum in red cells. Much this data dealt with phosphorylation serine residues. In study, we report detailed characterization tyrosine residues cell by P . Western blot analysis using anti‐phosphotyrosine anti‐phosphoserine antibodies 2‐DE conjunction double channel laser‐induced infrared fluorescence enabled accurate...
Background While G6PD deficiency is one of the major causes acute hemolytic anemia, membrane changes leading to red cell lysis have not been extensively studied. New findings concerning mechanisms deficient destruction may facilitate our understanding large individual variations in susceptibility pro-oxidant compounds and aid prediction activity new drugs. Methodology/Principal Findings Our results show that treatment cells with diamide (0.25 mM) or divicine (0.5 causes: (1) an increase...
Proteomics provides a powerful approach for screening alterations in protein expression and post-translational modification associated with particular human diseases. In this study, the analysis of was focused on malignant melanoma order to determine candidate genes involved tumour progression. The proteomes cultured melanocytes cell lines from primary metastatic lesions one patient were profiled using two-dimensional electrophoresis (2-DE) mass spectrometry. Differentially expressed...
Experimental data show that opiates interfere with calcium influx in the cell and some calcium-channel blockers are analgesic. We therefore studied effect of calcium-receptor blocker nifedipine on analgesic morphine rat, using tail-flick responses, humans, measurements intensity postoperative pain. In both experimental animals humans significantly (P less than 0.001) increased independently any metabolism morphine. Respiratory cardiovascular functions were not changed by nifedipine. The...
We have identified and sequenced a cDNA clone coding for Trichomonas vaginalis alpha-actinin. Analysis of the obtained sequence revealed that 2,857-nucleotide-long contained an open reading frame encoding 849 amino acids which showed consistent homology with alpha-actinins different species. Such was particularly significant in regions been reported to represent actin-binding Ca2+-binding domains other alpha-actinins. The deduced protein also characterized by presence divergent central...
Sickle cell disease (SCD) is an autosomal recessive genetic red disorder characterized by the production of a defective form hemoglobin, hemoglobin-S, that worldwide-distributed. The acute clinical manifestations SCD are related to hemoglobin cyclic-polymerization and generation rigid, dense blood cells (RBCs). We studied RBCs membrane proteome from human sickle RBCs, fractioned according density compared normal RBCs. 2-DE followed MS analysis was carried out. identified 65 proteins...
Protein tyrosine phosphatases (PTPs) are crucial components of cellular signal transduction pathways. Here, we report that red blood cells (RBCs) from mice lacking PTPepsilon (Ptpre(-/-)) exhibit (i) abnormal morphology; (ii) increased Ca(2+)-activated-K(+) channel activity, which was partially blocked by the Src family kinases (SFKs) inhibitor PP1; and (iii) market perturbation RBC membrane (Tyr-) phosphoproteome, indicating an alteration Using signaling network computational analysis...
Doxorubicin is commonly considered to exert its anti-tumor activity by triggering apoptosis of cancer cells through DNA damage. Recent reports have shown that elicits a marked heat shock response, and either inhibition or silencing proteins enhance the apoptotic effect in neuroblastoma cells. In order investigate whether may also act protein modification, we performed proteomic analysis ubiquitinated proteins. Here show nanomolar treatment caused: (a) dose-dependent over-ubiquitination...
Background Neuroblastic tumors account for 9–10% of pediatric and neuroblastoma (NB) is the first cause death in pre-school age children. NB classified four stages, depending on extent spreading. A fifth type NB, so-called stage 4S (S special), includes patients with metastatic but an overall survival that approximates 75% at five years. In most these cases, tumor regresses spontaneously regression probably associated delayed neuroblast cell differentiation. Methodology/Principal Findings...