A5000035191- Endometrial and Cervical Cancer Treatments
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Adipokines, Inflammation, and Metabolic Diseases
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Uterine Myomas and Treatments
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Circular RNAs in diseases
- Genetic factors in colorectal cancer
- Estrogen and related hormone effects
- Atherosclerosis and Cardiovascular Diseases
Haukeland University Hospital
2021-2025
University of Bergen
2020-2025
A major hurdle in translational endometrial cancer (EC) research is the lack of robust preclinical models that capture both inter- and intra-tumor heterogeneity. This has hampered development new treatment strategies for people with EC.EC organoids were derived from resected patient tumor tissue expanded a chemically defined medium. Established EC orthotopically implanted into female NSG mice. Patient corresponding characterized by morphological evaluation, biomarker gene expression whole...
The presence of cancer stem cells is linked to aggressive disease and higher risk recurrence, multiple markers have been proposed detect cells. However, a detailed evaluation the expression patterns prognostic value relevant for endometrial lacking. As organoid models are suggested be enriched in cells, such may prove valuable define tissue-specific To address this, imaging mass cytometry multiplex single-cell analyses were performed on an patient series including both tumor biopsies...
The endometrial cancer mismatch repair (MMR) deficient subgroup is defined by loss of MSH6, MSH2, PMS2 or MLH1. We compare MMR status in paired preoperative and operative samples investigate the prognostic impact differential protein expression levels.Tumour lesions from 1058 patients were immunohistochemically stained for was evaluated as intact to determine status, staining index evaluate potential expression. Gene data a local (n = 235) TCGA 524) cohorts used validation.We identified...
Abstract Background Precise preoperative risk classification of endometrial cancer is crucial for treatment decisions. Existing clinical markers often fail to accurately predict lymph node metastasis and recurrence risk. Loss vimentin expression has emerged as a potential marker predicting in low-risk patients. We assessed whether biopsies predicts poor prognosis large multicentre cohort. Methods Vimentin was evaluated using immunohistochemistry 1483 patients diagnosed with across 14...
Obesity is a disease characterized by chronic low-grade systemic inflammation and has been causally linked to the development of 13 cancer types. Several studies have undertaken determine whether tumors evolving in obese environments adapt differential interactions with immune cells this can be connected outcome. Most these limited single-cell lines tumor models analysis cell populations. Given multicellular complexity system its dysregulation obesity, we applied high-dimensional suspension...
Abstract Obesity is a disease characterized by chronic low-grade systemic inflammation and has been causally linked to the development of 13 cancer types. Several studies have undertaken determine if tumors evolving in obese environments adapt differential interactions with immune cells this can be connected outcome. Most these limited single cell lines tumor models analysis populations. Given multicellular complexity system its dysregulation obesity, we applied high-dimensional suspension...
<h3>Introduction/Background</h3> We recently identified low vimentin as a marker of recurrence in low-stage endometrial cancers (Lien et al. eBioMedicine, Volume 92, 2023). Such markers are currently lacking clinical use and could improve treatment for patients with undetected high-risk recurrence. aimed to validate the robustness poor prognosis cancer. <h3>Methodology</h3> Imaging mass cytometry was used examine single-cell expression 23 proteins 36 primary FIGO IB cancers, which 17...
Abstract Patients with localized endometrial carcinomas (FIGO IA and IB) have a 5-year relative survival rate of &gt; 90%. Still, some patients an expected good prognosis experience recurrence disease which dramatically reduces rates. Currently, we lack the knowledge to predict will get recurrence. We used Imaging Mass Cytometry examine 27 proteins in tissue microarrays 36 primary FIGO IB carcinomas, 17 later recurred. High dimensional images were segmented extract single-cell...
<h3>Objectives</h3> Patient-derived cancer organoids have quickly developed as valuable tools for drug testing they better represent the genetic background of patient cohort. We recently published a protocol establishing EC from all types and grades (Berg et al, Nat Comms Med 2021). here present data prospectively collected biobank including molecular subclasses. Models been extensively profiled evaluated response, supporting high heterogeneity in EC. <h3>Methods</h3> Organoids were derived...