A5101702761- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Spine and Intervertebral Disc Pathology
- Reproductive biology and impacts on aquatic species
- Musculoskeletal pain and rehabilitation
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Sexual Differentiation and Disorders
- Cervical and Thoracic Myelopathy
- Biological Activity of Diterpenoids and Biflavonoids
- Amoebic Infections and Treatments
- Turtle Biology and Conservation
- Shoulder Injury and Treatment
- Pain Management and Treatment
- Pluripotent Stem Cells Research
- Flavonoids in Medical Research
- Liver physiology and pathology
- Biological and pharmacological studies of plants
- Mitochondrial Function and Pathology
- Genomics, phytochemicals, and oxidative stress
- Ginkgo biloba and Cashew Applications
- Gender Studies in Language
- Sphingolipid Metabolism and Signaling
- Liver Disease Diagnosis and Treatment
- Tendon Structure and Treatment
- Biomedical Research and Pathophysiology
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
Nanjing Agricultural University
2022-2023
Chinese Academy of Fishery Sciences
2022-2023
Ministry of Agriculture and Rural Affairs
2022-2023
Hunan Normal University
2021
Beijing Center for Disease Prevention and Control
2021
Southwest Hospital
2016-2019
Army Medical University
2016-2018
Southwest University
2016
Background: Hexavalent chromium (Cr(VI)) overdose causes hepatocellular injuries by inducing mitochondrial damage and subsequent apoptosis in animals humans. Autophagy can selectively remove damaged organelles, especially impaired mitochondria, turn, protects against mitochondria-dependent cell death. The present study was designed to explore the effects of autophagy on Cr(VI)-induced hepatotoxicity. Methods: L-02 hepatocytes were incubated with different concentrations Cr(VI) for 24h...
Mesenchymal stem cell‐ (MSC‐) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it still challenge induce MSC differentiation in NP‐like cells when MSCs are implanted into NP. The purpose this study was construct poly(D,L‐lactide‐co‐glycolide) (PLGA) nanoparticles carriers TGF‐ β 3 controlled release and establish codelivery system dextran/gelatin hydrogel...
Introduction: Bone morphogenetic proteins (BMPs) play a crucial role in bone formation and differentiation. Recent RNA-Seq results suggest that BMPs may be involved the sex differentiation of P. sinensis, yet more relevant studies about sinensis are lacking. Methods: Herein, we identified BMP gene family members, analyzed phylogeny, collinear relationship, scaffold localization, structures, protein transcription factors dimorphic expression by using bioinformatic methods based on genomic...
Background/Aims: Matrix homeostasis within the disc nucleus pulposus (NP) tissue is important for function. Increasing evidence indicates that sex hormone can influence severity of degeneration. This study was aimed to role 17β-estradiol (E2) in NP matrix synthesis and its underlying mechanism. Methods: Rat cells were cultured with (10-5, 10-7 10-9 M) or without (control) E2 for48 hours. The estrogen receptor (ER)-β antagonist PHTPP ERβ agonist ERB 041 used investigate mediated by ERβ. p38...
Mesenchymal stem cell- (MSC-) based therapy is regarded as a promising tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it still challenge promote biosynthesis MSC meet requirement NP regeneration. The purpose this study was optimize compressive magnitude enhance extracellular matrix (ECM) deposition towards discogenesis MSCs. Thus, we constructed 3D culture model MSCs bear different magnitudes...
In aquaculture, the Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important species with remarkable gender dimorphism in its growth patterns. However, underlying molecular mechanisms of this phenomenon have not been elucidated well. Here, we conducted a whole-transcriptome analysis female and male gonads P. sinensis. Overall, 7833 DE mRNAs, 619 lncRNAs, 231 circRNAs, 520 miRNAs were identified. Some "star genes" associated sex differentiation containing dmrt1, sox9,...
There was a controversy about surgery approach of thoracic and lumbar tuberculosis (TB) treatment. The aim this study to compare the microbiology outcomes drainage liquid clinical posterior anterior in treatment TB. A total 105 patients were enrolled prospective from February 2011 September 2015. Patients divided into two groups: group (51 patients, surgery) B (54 surgery). Intraoperative TB samples sent for Mycobacterium culture (MTBC). Drainage fluid postoperatively collected polymerase...
Long-term exposure to a mechanical load causes degenerative changes in the disc nucleus pulposus (NP) tissue. A previous study demonstrated that N-cadherin (N-CDH)-mediated signalling can preserve NP cell phenotype. However, N-CDH expression and resulting phenotype alteration cells under compression remain unclear. The present investigated effects of compressive duration on an
Background: Hexavalent chromium (Cr(VI)) is a common environmental pollutant. Cr(VI) exposure can lead to severe damage in the liver, but preventive measures diminish Cr(VI)-induced hepatotoxicity need further study. Acid sphingomyelinase (ASMase) responsible for production of ceramide via hydrolysis sphingomyelin. The present study was designed investigate effects desipramine (DES), as an ASMase inhibitor, on hepatotoxicity. Methods: L-02 hepatocytes were incubated with different...
In aquaculture, the traditional method for hatching Pelodiscus sinensis eggs needs further investigation. Here, we developed a medium-free incubation P. and evaluated its differences from vermiculite effects of light on it. We found that had no significant success, body weight, carapace length, width, plastron righting response time compared with those under incubation, irrespective thermostatic or fluctuating temperature conditions. Furthermore, treatment significantly shortened period...
Nanog is a homeodomain-containing transcription factor, and it plays vital role in maintaining the pluripotency of embryonic stem cells. Nanog's function has been well studied many species. However, there lack reporting on gene reptile. Here, we identified 1032 bp cDNA sequence Pelidiscus sinensis, known as PsNanog. PsNanog highly conserved HD domain shares high identity with that Chelonia mydas lowest Oryzias latipes. Similarly, presented tight cluster C. Nanog, but was far from those...
Cisplatin (DDP)‐based chemotherapy is a preferred treatment for broad spectrum of cancers, but the precise mechanisms its hepatotoxicity are not yet clear. Recently, role voltage‐dependent anion channel protein 1 (VDAC1) in mitochondrial activity and cell apoptosis has attracted much attention. Our aim was to investigate effects outer membrane VDAC1 oligomerization DDP‐induced hepatocyte apoptosis. L‐02 hepatocytes were divided into 4 groups: (a) control group, (b)...
The Barbodes hexagonolepis, as an important commercial species, had the limited resources on biology and genetics. We reported complete mitogenome of this got a whole length 16 587 bp (containing two rRNAs, 22 tRNAs, 13 mRNAs one D-loop region). rRNAs located between tRNAphe tRNAleu were separated by tRNAval. tRNAs was ranged from 66 (tRNAcys) to 76 (tRNAleu tRNAlys) in length. four overlap regions, three types start codons termination codons. region found tRNAPro tRNAPhe. To further explore...
Spats1 (spermatogenesis-associated, serinerich 1) has been characterized as a male-biased gene which acts an important role in the germ cell differentiation of mammals. Nevertheless, function Chinese soft-shelled turtle (P. sinensis) not yet reported. To initially explore expression P. sinensis and its response to sex steroid treatment, we cloned CDS for first time analyzed profile different tissues, including testes seasons. The cDNA fragment is 1201 base pairs (bp) length contains open...