A5061227090- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- Biosensors and Analytical Detection
- Polymer Surface Interaction Studies
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Advanced Sensor and Energy Harvesting Materials
- Hydrogels: synthesis, properties, applications
- RNA Interference and Gene Delivery
- Supramolecular Self-Assembly in Materials
- Microfluidic and Capillary Electrophoresis Applications
- Cancer Immunotherapy and Biomarkers
- Polydiacetylene-based materials and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Advanced Nanomaterials in Catalysis
- Molecular Sensors and Ion Detection
- SARS-CoV-2 detection and testing
- Advanced Materials and Mechanics
- Micro and Nano Robotics
- Graphene and Nanomaterials Applications
Ocean University of China
2023-2025
Hong Kong Polytechnic University
2021-2024
Qingdao National Laboratory for Marine Science and Technology
2024
Chinese University of Hong Kong
2016-2022
Shatin Hospital
2017
State Council of the People's Republic of China
2017
Abstract 3D culture of cells in designer biomaterial matrices provides a biomimetic cellular microenvironment and can yield critical insights into behaviours not available from conventional 2D cultures. Hydrogels with dynamic properties, achieved by incorporating either degradable structural components or reversible crosslinks, enable efficient cell adaptation the matrix support associated functions. Herein we demonstrate that given similar equilibrium binding constants, hydrogels containing...
Abstract Immunotherapy has revolutionized oncology remarkably and gained great improvements in cancer therapy. However, tumor immunotherapy still encounters serious challenges, especially certain tumors barely respond to immunotherapy. The lack of immunogenicity subsequent insufficient antitumor immune activation is a pivotal reason. Here, general introduction the strengthening strategies for enhanced are reviewed. Specifically, nanotechnology nowadays playing important roles increasing...
Abstract Surface‐enhanced Raman scattering (SERS) imaging has emerged as a promising tool for guided cancer diagnosis and synergistic therapies, such combined chemotherapy photothermal therapy (chemo‐PTT). Yet, existing therapeutic agents often suffer from low SERS sensitivity, insufficient conversion, or/and limited drug loading capacity. Herein, multifunctional theragnostic nanoplatform consisting of mesoporous silica‐coated gold nanostar with cyclic Arg‐Gly‐Asp (RGD)‐coated nanocluster...
Abstract With the advancement of medical and digital technologies, smart skin adhesive patches have emerged as a key player for complex purposes. In particular, with integrated electronics created an excellent platform monitoring health conditions intelligent medication. However, efficient design is still challenging it requires strong combination network structure, adhesion, physical properties, biocompatibility. To assimilated device, one must deep knowledge various patches. This article...
Hydrogels have emerged in various biomedical applications, including tissue engineering and medical devices, due to their ability imitate the natural extracellular matrix (ECM) of tissues. However, conventional static hydrogels lack dynamically respond changes surroundings withstand robust biophysical microenvironment trigger on-demand functionality such as drug release mechanical change. In contrast, multifunctional dynamic can adapt external stimuli drawn great attention recent studies. It...
Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment cell-based therapies. Nevertheless, limitations conventional inorganic PA contrast agents narrow range excitation wavelength first near-infrared (NIR-I) window hamper applications living subjects. Herein, we report design synthesis a second (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for cells. Comparison studies biological tissue show that NIR-II...
Cells sense and respond to the surrounding microenvironment through binding of membranous integrin ligands such as Arg-Gly-Asp (RGD) peptide. Previous studies show that RGD tether properties on substrate influence cell adhesion spreading, but few have reported strategies control mobility via a physical noncontact approach. Herein, we demonstrate novel strategy tune magnetic force. We conjugate monolayer RGD-bearing nanoparticles (MNPs) glass flexible coiled poly(ethylene glycol) linker large...
Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) improved efficiency. We further investigate impact of dynamic conformational change SCNGs on cellular interactions from to bulk scale. First, supramolecular unfoldable efficiently deliver siRNAs into stem cells as a drug carrier in conformation-dependent manner. Furthermore, conformation changes enable...
CRISPR-Cas12a has been integrated with nanomaterial-based optical techniques, such as surface-enhanced Raman scattering (SERS), to formulate a powerful amplification-free nucleic acid detection system. However, nanomaterials impose steric hindrance limit the accessibility of narrow gaps (SERS hot spots) among nanoparticles (NPs) for producing significant change in signals after detection.
Surface-enhanced Raman scattering (SERS)-based biosensors are promising tools for virus nucleic acid detection. However, it remains challenging SERS-based using a sandwiching strategy to detect long-chain acids such as nucleocapsid (N) gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because the extension coupling distance (CD) between two tethered metallic nanostructures weakens electric field and SERS signals. Herein, we report magnetic-responsive substrate consisting...
Abstract The ongoing outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) pandemic has posed significant challenges in early viral diagnosis. Hence, it is urgently desirable to develop a rapid, inexpensive, and sensitive method aid point‐of‐care SARS‐CoV‐2 detection. In this work, we report highly sequence‐specific biosensor based on nanocomposites with aggregation‐induced emission luminogens (AIEgen)‐labeled oligonucleotide probes graphene oxide nanosheets (AIEgen@GO)...
Macrophages play crucial roles in various immune-related responses, such as host defense, wound healing, disease progression, and tissue regeneration. perform distinct dynamic functions vivo, depending on their polarization states, the pro-inflammatory M1 phenotype pro-healing M2 phenotype. Remote manipulation of adhesion macrophages to implants subsequent vivo can be an attractive strategy control macrophage polarization-specific but has rarely been achieved. In this study, we grafted RGD...
Mussel-mimetic hydrogels possessing ultrahigh adhesion energy on wet biological tissues <italic>via</italic> enhancing both the interfacial and bulk cohesion are fabricated.
Remote, noninvasive, and reversible control over the nanoscale presentation of bioactive ligands, such as Arg-Gly-Asp (RGD) peptide, is highly desirable for temporally regulating cellular functions in vivo. Herein, we present a novel strategy physically uncaging RGD using magnetic field that allows safe deep tissue penetration. We developed heterodimeric nanoswitch consisting nanocage (MNC) coupled to an underlying RGD-coated gold nanoparticle (AuNP) via long flexible linker. Magnetically...
Macrophages are key immune cells that perform various physiological functions, such as the maintenance of homeostasis, host defense, disease progression, and tissue regeneration. adopt distinctly polarized phenotypes, pro-inflammatory M1 phenotype or anti-inflammatory (pro-healing) M2 phenotype, to execute disparate functions. The remotely controlled reversible uncaging bioactive ligands, Arg-Gly-Asp (RGD) peptide, is an appealing approach for temporally regulating adhesion resultant...
Cellular adhesion is regulated by the dynamic ligation process of surface receptors, such as integrin, to adhesive motifs, Arg-Gly-Asp (RGD). Remote control ligand presentation using external stimuli an appealing strategy for temporal regulation cell-implant interactions in vivo and was recently demonstrated photochemical reaction. However, limited tissue penetration light potentially hampers widespread applications this method vivo. Here, we present a modulating nanoscale oscillations...
Abstract Remote control of stem cell differentiation in vivo by stimuli‐responsive nanomaterials with the use tissue‐penetrative stimuli is an appealing strategy for versatile regulation therapy. In this study, upconversion nanotransducer (UCNT)‐based nanocomplex photolabile caging chondro‐inductive kartogenin (KGN) and/or either calcium chelator or supplier (caged calcium), and subsequent coupling integrin‐binding ligand via cyclodextrin‐adamantine supramolecular complexation utilized....
Material implants trigger host reactions generated by cells, such as macrophages, which display dynamic adhesion and polarization including M1 inflammatory state M2 anti-inflammatory state. Creating materials that enable diverse nanoscale of integrin-binding groups, RGD ligand, can unravel recruitment ligation integrin, modulate cellular activation. Here, we synthesized gold nanorods (GNRs) with various anisotropies (i.e., aspect ratios, ARs), but in similar surface areas, controlled their...
Biophysical cues of rigid tumor matrix play a critical role in cancer cell malignancy. We report that stiffly confined cells exhibit robust growth spheroids the stiff hydrogel exerts substantial confining stress on cells. The stressed condition activated Hsp (heat shock protein)–signal transducer and activator transcription 3 signaling via transient receptor potential vanilloid 4–phosphatidylinositol 3-kinase/Akt axis, thereby up-regulating expression stemness-related markers cells, whereas...
Monitoring T lymphocyte differentiation is essential for understanding cell fate regulation and advancing adoptive immunotherapy. However, current biomarker analysis methods necessitate lysis, leading to source depletion. Intracellular pH (pHi) can be affected by the presence of lactic acid (LA), a metabolic mediator activity such as glycolysis during activation; therefore, it potentially good state. In this work, dual emitting enhancement-based nanoprobe, namely, AIEgen@F127-AptCD8, was...