A5086017994- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cancer Cells and Metastasis
- Polyomavirus and related diseases
- vaccines and immunoinformatics approaches
- Reproductive System and Pregnancy
- T-cell and B-cell Immunology
- Viral-associated cancers and disorders
- RNA Interference and Gene Delivery
- Plant Virus Research Studies
- Peptidase Inhibition and Analysis
- Cytomegalovirus and herpesvirus research
- Cancer-related gene regulation
- Computational Drug Discovery Methods
- Neonatal Respiratory Health Research
- Hematopoietic Stem Cell Transplantation
- Genital Health and Disease
- Retinoids in leukemia and cellular processes
- Circular RNAs in diseases
Universidad Nacional Autónoma de México
2002-2025
Universidad de Zaragoza
2006-2024
Mayo Clinic
1999
WinnMed
1999
In recent years, immunomodulatory mechanisms of mesenchymal stem/stromal cells (MSCs) from bone marrow and other "classic" sources have been described. However, the phenotypic functional properties tumor MSCs are poorly understood. The aim this study was to analyze immunosuppressive capacity cervical cancer-derived (CeCa-MSCs) on effector T lymphocytes through purinergic pathway.We determined expression activity membrane-associated ectonucleotidases CD39 CD73 CeCa-MSCs normal tissue-derived...
Abstract Background Even though two prophylactic vaccines against HPV are currently licensed, infections by the virus continue to be a major health problem mainly in developing countries. The cost of limits wide-scale application poor A promising strategy for producing affordable and efficient involves expression recombinant immunogens plants. Several genes have been expressed plants, including L1, which can self-assemble into virus-like particles. plant-based, dual prophylactic/therapeutic...
The presence of TGF-β in the tumor microenvironment cervical cancer (CC) is important for progression. In this study, we analyzed effect on expression ectonucleotidases CD39 and CD73, which are involved generation adenosine (Ado), CC cells extracellular vesicles (EVs) secreted by these cells. Treatment HeLa CaSki 72 h with recombinant human increased CD73 20 30% 40 100%, respectively. addition SB505124, an inhibitor TGF-β1 receptor, or GW4869, exosome formation release, reduced release both...
DNA hypermethylation and histone deacetylation are epigenetic events that contribute to the absence or downregulated expression of different components tumor recognition complex. These affect processing presentation antigenic peptides CTLs by HLA class-I molecules. In this work evaluated effect hypomethylating agent hydralazine deacetylase inhibitor valproic acid, on molecules antigen-specific immune cervical cancer cells. Cell lines C33A (HPV-), CaSki (HPV-16+) MS751 (HPV-18+) were treated...
Mesenchymal stromal cells (MSCs) have been isolated from different tumors and it has suggested that they support tumor growth through immunosuppression processes favor cell evasion the immune system. To date, however, presence of MSCs in cervical cancer (CeCa) their possible role remains unknown. Herein we report on tissue, both normal conditions (NCx-MSCs) CeCa (CeCa-MSCs), described several biological properties such cells. Our study showed similar patterns surface antigen expression, but...
The aetiological relationship between human papillomavirus (HPV) infection and cervical cancer (CC) is widely accepted. Our goal was to determine the prevalence of HPV types in Mexican women attending at Institute for Social Security from different areas Mexico.DNAs 2,956 samples were subjected genotyping: 1,020 with normal cytology, 931 low-grade squamous intraepithelial lesions (LGSIL), 481 high grade HGSIL 524 CC.Overall 67.1%. A total 40 found; HPV16 detected 39.4% HPV-positive followed...
The present study provides evidence showing that adenosine (Ado) increases the expression of programmed death ligand 1 (PD-L1) in cervical cancer (CeCa) cells by interacting with A2AR/A2BR and TGF-β1 acts an autocrine manner to induce PD-L1 expression, enhancing immunosuppressive effects CeCa on activated T lymphocytes (ATLs) CD8+ cytotoxic (CTLs) specific for antigenic peptides derived from E6 E7 proteins HPV-16. Interestingly, addition antagonists ZM241385 MRS1754, which are A2AR A2BR,...
Abstract Mesenchymal stromal cells (MSCs) together with malignant present in the tumor microenvironment (TME), participate suppression of antitumor immune response through production immunosuppressive factors, such as transforming growth factor beta 1 (TGF‐β1). In previous studies, we reported that adenosine (Ado), generated by adenosinergic activity cervical cancer (CeCa) cells, induces TGF‐β1 interacting A 2A R/A 2B R. study, provide evidence Ado MSCs derived from CeCa tumors (CeCa‐MSCs)...
Mesenchymal stromal cells (MSCs) in the tumor microenvironment (TME) participate together with to suppress antitumor effector through production of immunosuppressive factors, such as transforming growth factor-beta 1 (TGF-β1). Furthermore, TGF-β1 can induce 5'-nucleotidase (CD73) expression various cell types; this functional activity is associated adenosine (Ado), which an nucleoside. In study, we provide evidence that coculture MSCs derived from cervical tumors (CeCa-MSC) CeCa increases...
Recently, a link between the biological activity of CD73 and tumorigenicity in solid tumors has been proposed. We previously reported that generation adenosine (Ado) by cervical cancer (CC) cells induces transforming growth factor-beta 1 (TGF-β1) production to maintain expression. In present study, we analyzed participation TGF-β1 expression development protumoral characteristics CaSki CC cultured as tumorspheres (CaSki-T) monolayers (CaSki-M). Compared with those CaSki-M cells, Ado ability...
The development of cervical cancer (CeCa) is associated with high-risk human papilloma virus (HR-HPV) infections, mainly HPV-16, which present in more than 50% cases. presence immunosuppressive factors the early stages disease also strongly linked to CeCa progression. In this context, it unknown whether ectonucleotidases CD39 and CD73, are involved production adenosine (Ado) that suppresses specific antitumor immune response, precursor lesions CeCa. pilot study, we analyzed CD73 their...
Interleukin 2 (IL-2) has been used for the treatment of different types cancer that express IL-2 receptor (IL-2R). However, effect on cervical cells is unknown. IL-2R present in normal immune system but not healthy cervix. We report expressed cells. decreases cell proliferation via transient arrest G1 phase, which does result apoptosis or senescence. upregulates expression p53 and p21 downregulates cyclin D. In addition, we resistance to treatments induce HeLa INBL When arrested were treated...
Recently, a link between the biological activity of CD73 in solid tumors and multidrug resistance protein (MRP) has been proposed. Cisplatin (CP) is most widely used anticancer agent to treat advanced recurrent cervical cancer (CC). However, protein-1 (MRP1) overexpressed approximately 85% these strongly associated with cisplatin (CPR). In this study, we examine involvement interaction adenosine (ADO) its receptors (ARs) MRP1 expression CC cells. We found that ADO positively modulates cells...
A nonapeptide (16L1) was derived from the human papillomavirus type 16 (HPV-16) major capsid protein and tested for detection of potential cross-reactive serum IgG cervical IgA antibodies in low- high-risk HPV-associated low-grade squamous intraepithelial lesions (LSIL) cancer patients by ELISA. The response similar women with low-risk LSIL controls (P=0.1). In contrast, more than 90 % were seropositive. Although tumours all positive presence HPV DNA, level seropositivity decreased...
Human dental tissue mesenchymal stem cells (DT-MSCs) constitute an attractive alternative to bone marrow-derived (BM-MSCs) for potential clinical applications because of their accessibility and anti-inflammatory capacity. We previously demonstrated that DT-MSCs from pulp (DP-MSCs), periodontal ligaments (PDL-MSCs), gingival (G-MSCs) show immunosuppressive effects similar those BM, but date, the DT-MSC-mediated immunoregulation T lymphocytes through purinergic pathway remains unknown. In...
The presence of IgG antibodies to HPV-16 L1-virus like particles (VLPs) in serum has been reported as a result persistent exposure the virus and marker disease progression. However, detection VLP-specific sera does not always indicate malignant lesion positive results may also be due nonmalignant viral infection. Furthermore, lesions are associated with an increased antibody titer for E6 E7 proteins. aim this study was develop ELISA using novel chimeric virus-like particle (cVLP) encoding L1...
Persistent infection with high-risk human papillomavirus (HR-HPV) is the main factor in development of cervical cancer (CC). The presence immunosuppressive factors plays an important role this type cancer. To determine whether CD39 and CD73, which participate production adenosine (Ado), are involved progression CC, we compared concentrations hydrolytic activity these ectonucleotidases platelet-free plasma (PFP) samples between patients low-grade squamous intraepithelial lesions (LSILs) (...