Vascular precursor cells with angiogenic potentials are important for tissue repair, which is impaired in diabetes mellitus. MicroRNAs recently discovered key regulators of gene expression, but their role vascular cell-mediated angiogenesis mellitus unknown. We tested the hypothesis that microRNA miR-27b rescues bone marrow-derived cell (BMAC) function vitro and vivo type 2 diabetic mice.BMACs from adult male db/db normal littermate db/+ mice were used. expression was decreased BMACs. mimic...

Abstract Pyroptosis participates in the formation and development of atherosclerosis (As) by promoting inflammatory factor release is closely related to stability atherosclerotic plaque. MicroRNAs can regulate expression target genes at posttranscriptional level. Previous studies have shown that miR‐125a‐5p increases hyperlipidemic–hyperglycemic conditions involved apoptosis, but its specific role pyroptosis As remains unclear. We propose may be implicated oxidized low‐density lipoprotein...

Abstract Trimethylamine N‐oxide (TMAO) is produced from the phosphatidylcholine metabolism of gut flora and acts as a risk factor cardiovascular disease. However, underlying mechanisms for its proatherogenic action remain unclear. This study aimed to observe effect TMAO on endothelial cell pyroptosis explore mechanisms. Our results showed that promoted progression atherosclerotic lesions in apolipoprotein E‐deficient (apoE −/− ) mice fed high‐fat diet. Pyroptosis succinate dehydrogenase...

The aim of this study was to investigate the effect chromosomal polymorphic variations on outcome IVF and embryo transfer (IVF–embryo transfer) treatment for infertile couples. During period from October 2006 December 2009, 1978 couples who had received their first IVF–embryo cycle in our hospital were selected retrospective study, frequency calculated. From these, 1671 divided into three groups: 1402 with normal chromosomes (Group 1/control group), 82 only females 2) 187 males 3). clinical...

Fibroblast growth factor 21 (FGF21) is a hormone-like member of the FGF family that associated with cell death in atherosclerosis. However, its underlying mechanisms remain unclear. In this study, effect FGF21 on endothelial pyroptosis and potential were investigated. Results showed inhibits oxidized low-density lipoprotein (ox-LDL)-induced related molecular expression human umbilical vein cells (HUVECs). Mitochondrial function was damaged by ox-LDL restored FGF21. A mechanism proved...

The present study aimed to explore the role and mechanisms of proprotein convertase subtilisin/kexin type 9 (PCSK9) in oxidized low‑density lipoprotein (oxLDL)‑induced pyroptosis vascular endothelial cells. For this purpose, human umbilical vein cells (HUVECs) were incubated with oxLDL (100 µg/ml) for 24 h induce pyroptosis, which was detected using PI/hoechst33342 double staining. expression pyroptosis‑associated molecules measured by western blot analysis RT‑qPCR. Reactive oxygen species...

<i>Objectives:</i> Berberine (BR) has a beneficial effect on endothelial function by increasing nitric oxide (NO), as NO plays pivotal role in the regulation of progenitor cell (EPC) mobilization and function. The aim present study was to investigate whether BR-induced upregulation circulating EPCs is related production healthy subjects. <i>Methods:</i> Twenty volunteers were recruited received 400 mg BR 3 times day for 30 days. We assessed number EPC colony-forming...

Trimethylamine-N-oxide (TMAO) has been proven to be a new proatherogenic compound for promoting inflammation and endothelial dysfunction. Hepatocyte-derived exosomes (Exos), including those derived from hepatocytes, play pivotal role in the regulation of function. As TMAO is produced liver, hepatocytes may potential target TMAO. However, it not yet clear whether can directly stimulate produce Exos mediate detrimental effects on vascular cells (VECs).Hepatocytes treated with (TMAO-Exos) were...

Atherosclerosis (AS) is a chronic inflammatory disease that involves cell death and endothelial dysfunction. Melatonin an endocrine hormone with anti-inflammatory anti-AS effects. However, the underlying molecular mechanisms for effects of melatonin are unknown. A previous study has shown pyroptosis plays detrimental role in development AS, therefore, this was designed to investigate anti-pyroptotic potential atherosclerotic endothelium. Our results show attenuated expression genes related...

Abstract Aim This study aimed to investigate the effect of high glucose (HG) level on proliferation, migration and invasion trophoblasts determine role placental growth factor (PLGF) in process. Methods HTR8‐S/Vneo was treated with different concentrations d ‐glucose (0, 10, 15, 20, 25 30 μM) at times 6, 12 24 h). qRT‐PCR Western blot analyses were used measure PLGF expression. The protein measured by immunofluorescence. Cell proliferation assessed CCK‐8 analysis. Wound healing transwell...

Abstract Introduction Hyperglycemia is closely related to trophoblast dysfunction during pregnancy and results in suppressed invasion, migration, pro‐inflammatory cell death of trophoblasts. a dependent risk factor for gestational hypertension accompanied by decreased placental growth (PLGF), which important maternal fetal development. However, there currently lack evidence support whether PLGF can alleviate caused high blood sugar. Here, we aim clarify the effect hyperglycemia on determine...

Summary Polymorphisms of transferrin (Tf), pre‐albumin (Pa), haemopexin (Hpx), ceruloplasmin (Cp) and amylase (Am) Duroc pigs Hunan indigenous were investigated using horizontal starch gel electrophoresis. Allele frequencies Durocs determined in 2004 compared with presented the paper by Baker L.N. (1968) Serum protein variation Hampshire pigs. Vox Sanguinis 15 , 154–8. The number serum alleles decreased over time allele aggregated across certain alleles, including Tf B Pa A Hpx 3 Cp Am ....

Background A functional -94 insertion/deletion polymorphism (rs28362491) in the promoter of NFKB1 gene was reported to influence expression and confer susceptibility different types cancer. This study aims determine whether is associated with risk bladder

Objective To observe the expression and transcription of MART-1 in human uveal melanoma cell lines 92-1, 92-2, Ocm3, Me1285, as well possible effect methylation on its expression. Methods The Ocm3 Mel285 were cultured routinely tested for at protein mRNA level by FACS analysis, Western blot RT-PCR respectively. Methylation status promoter region all checked Southern blots DNA digested with sensitive restriction enzymes. Results As observed analysis blot, 92-2 positive while...