A5019790515- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Urinary Bladder and Prostate Research
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Pediatric Urology and Nephrology Studies
- Ferroptosis and cancer prognosis
- Liver Diseases and Immunity
- Urological Disorders and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Multiple and Secondary Primary Cancers
- Lipid metabolism and disorders
- Cancer Mechanisms and Therapy
- Planarian Biology and Electrostimulation
- Oral Health Pathology and Treatment
- Genomics, phytochemicals, and oxidative stress
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immune cells in cancer
- Air Quality and Health Impacts
- Inflammasome and immune disorders
- Genital Health and Disease
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Cholinesterase and Neurodegenerative Diseases
- Extracellular vesicles in disease
Jiaxing University
2020-2024
First Hospital of Jiaxing
2000-2021
Ningxia Medical University
2021
Chongqing Technology and Business University
2017
Guangdong Medical College
2008-2009
Abstract MAFG-AS1 is an oncogenic lncRNA in multiple types of cancer. However, its role bladder cancer (BC) remains unclear. The present study aimed to investigate the function BC. BC and paired non-tumor tissues were collected. Two cell lines HT01197 HT-1376 used. Dual luciferase activity assay, RT-qPCR, western blot, CCK-8, transwell invasion wound healing assay performed. We found that was significantly up-regulated predicted a poor survival rate. interacted with miR-125b-5p. expression...
Gemcitabine (GEM) is one of the first choice drugs for treating bladder cancer. In this study, we loaded M1 macrophage-derived exosomes (M1-Exo) with GEM by ultrasonication technique to derive an M1-Exo-GEM drug delivery system, and then explored its effects on After inducing polarization macrophages in vitro, ultracentrifugation was performed obtain M1-Exo, followed construction via technique. Mouse cancer MB49 cells were chosen study. CCK-8, PI staining flow cytometry (FCM) assays employed...
Abstract We explored the role and mechanism of cordycepin (COR) in inhibiting kidney injury. A mouse model injury was established using cisplatin (CDDP), function, histopathology, ferroptosis indices mice were detected after intervening with COR. The targets COR‐ferroptosis‐kidney analyzed by network pharmacology, based on which association between glycogen synthase kinase‐3 beta (GSK‐3β) COR determined. HK‐2 cells cultured vitro treated separately inducers erastin CDDP. After intervention,...
Abstract Background Population-based analysis for the short-term non-bladder cancer related mortality among patients with non-metastatic bladder is currently lacking. The objective of current study was to assess and quantify cause death after diagnosis. Methods custom Surveillance, Epidemiology, End Results (SEER) dataset standardized ratios (SMRs) utilized identify 24,074 who were diagnosed nonmetastatic (M0) from 2014 2015. SMRs causes calculated. Risk factors cancer-specific mortality,...
We reveal the mechanism of action whereby ambient PM2.5 promotes kidney injury. Using C57BL/6 mice, effects exposure on acute injury (AKI) were investigated, including renal function changes, expression inflammatory cytokines, histopathological as well activation nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3(NLRP3). The after NLRP3 inhibition explored using inhibitor (MCC950) knockout mice. response macrophages investigated at cellular level....
Abstract To explain the effect and mechanism of cordycepin (COR) in resisting acute kidney injury (AKI). Network pharmacology was employed to analyze correlations between COR, AKI, pyroptosis, as well action target COR. A mouse model AKI established by ischemia reperfusion (IRI), after treatment with renal function, tissue inflammatory cytokine levels, pyroptosis‐related signals were detected mice. In in‐vitro experiments, damage macrophages caused oxygen‐glucose deprivation model,...
To explore the role of CTHRC1 in regulating proliferation and apoptosis papillary thyroid cancer cells.Papillary TPC-1 cells were transfected with a small interfering RNA (siRNA) targeting CTHRC1, scrambled sequence as negative control. The changes cell assessed using counting kit-8 (CCK-8) flow cytometry AV/PI double staining, respectively. expression c-caspase-3, c-PARP1 phosphorylation ERK1/2 examined Western blotting.Transfection siRNA significantly decreased mRNA protein levels TCP-1 (P...
Objective To investigate the role of MMPs expression in invasion and metastasis bladder cancer. Methods 50 patients with cancer were selected as observation group. 30 healthy people control ELISA method was used to detect serum contents MMP-2 MMP-9. The distribution MMP-9 detected by immunohistochemical SP method. results two groups compared. Results The levels between lymph node group had statistically significant differences(t=7.532, 6.358, all P<0.05), differences without...
Long noncoding RNAs play an important regulatory role in the development and progression of tumors. Our study found that LINC00478 was upregulated clear cell renal carcinoma (ccRCC), so we made in-depth exploration into its mechanism. In Caki-2 cells, established oe-LINC00478 line overexpressing LINC00478, underexpressing sh-LINC00478 by short hairpin RNA silencing. The abilities invasion metastasis were significantly enhanced, proliferative potential also improved. cellular expressions...
Objective To describe and evaluate the technique outcome of transperitoneal left laporoscopic pyeloplasty with transmesocolic approach to ureteropelvic junction(UPJ). Meth-ods Twelve patients UPJ obstruction were enrolled. After entering peritoneal cavity, a win-dow was made into descending mesocolon in an avascular area no colon reflection. Ureter dissected through this window stented dismembered done. The operative time, blood loss, time resumption oral intake complications, hospital stay...
Objective To investigate the effect of nuclear factor (NF)-κBp65 antisense oligonucleotide (ASODN) on NF-κB activity and expression interleukin(IL)-6 in hepatic stellate cells (HSC). Methods The HSC were separated from rats cultured. toxicity NF-κBp65 ASODN detected by Trypan blue exclusion staining was determined EMSA. expressions IL-6 mRNA protein meaured RT-PCR ELISA, respectively. Results In vitro, no observed at concentrations 0. 001 to 1.0 μmol/L. increased after stimulating with tumor...
A 48-year-old male was suffered from slowly progressive dysuria over twenty years. Although multiple urethral dilatation treated, the effect unsatisfactory. He diagnosed with penile lichen sclerosus related to stricture due lichenification of glans penis, stenosis meatus, and long anterior shown by urethrography. Finally, patient underwent an enlarged urethroplasty lingual mucosal graft (17 cm in length), obtained a good outcome. During two-year postoperative follow-up, maintained...