A5102021869- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Phytochemistry and biological activity of medicinal plants
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Pharmacological Effects of Natural Compounds
- Circular RNAs in diseases
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- Bioactive Compounds and Antitumor Agents
- Cancer-related gene regulation
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Advanced Breast Cancer Therapies
- Kruppel-like factors research
- Skin Protection and Aging
- Traditional Chinese Medicine Analysis
- Tumors and Oncological Cases
- PARP inhibition in cancer therapy
- Peptidase Inhibition and Analysis
- Natural Compound Pharmacology Studies
University of Electronic Science and Technology of China
2020-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2020
First Affiliated Hospital of Nanchang University
2019
Nanchang University
2019
Beijing Hospital
2011-2015
Shanghai Zhangjiang Laboratory
2013
University of Leeds
2010
Louisiana State University
2010
University of Michigan–Ann Arbor
1999-2002
Beta-Catenin has a critical role in E-cadherin-mediated cell-cell adhesion, and it also functions as downstream signaling molecule the wnt pathway. Mutations putative glycogen synthase kinase 3beta phosphorylation sites near beta-catenin amino terminus have been found some cancers cancer cell lines. The mutations render resistant to regulation by complex containing 3beta, adenomatous polyposis coli, axin proteins. As result, accumulates cytosol nucleus activates T-cell factor/ lymphoid...
The Drosophila seven in absentia (sina) gene was initially discovered because its inactivation leads to R7 photoreceptor defects. Recent data indicate that Sina binds the Sevenless pathway protein Phyllopod, and together they mediate degradation of Tramtrack, a transcriptional repressor cell fate. Independent studies have shown highly related mammalian homologues Siah-1 Siah-2 bind DCC (deleted colorectal cancer) promote proteolysis via ubiquitin-proteasome pathway. To determine roles Siahs...
The bell-shaped rate−pH curve coupled to production of base in the urea−urease reaction was utilized give feedback-driven behavior: an acid-to-base pH clock (a kinetic switch), bistability and hysteresis between acid/base state when initial adjusted by a strong acid, aperiodic oscillations weak acid open reactor. A simple model reproduced most experimental results provided insight into role self-buffering dynamics. This suggests new possibilities development biocompatible feedback couple...
Abstract Purpose To evaluate the efficacy and safety of pamiparib in patients with locally advanced or metastatic human epidermal growth factor receptor 2-negative (HER2−) breast cancer, deleterious suspected germline BRCA1/2 mutations (g m). Methods In this open-label, phase II, multicenter study China (NCT03575065), triple-negative cancer (TNBC cohort) hormone receptor-positive (HR+)/HER2− (HR+/HER2− ≤ 2 prior lines chemotherapy received 60 mg orally twice daily 28-day, continuous cycles....
Reducing the expression of endothelial cell adhesion molecules (ECAMs) is known to decrease inflammation-induced vascular complications. In this study, we explored whether rhein can reduce ECAMs in human umbilical vein cells (HUVECs) with or without lipopolysaccharide (LPS) stimulation. HUVECs were treated different concentrations 2.5 μg/ml LPS Cell viability was assayed using MTT method. Real-time PCR and Western blot analysis used measure transcription levels ECAMs, including intercellular...
Gambogic acid (GA) inhibits the proliferation of various human cancer cells. However, because its water insolubility, antitumor efficacy GA is limited. Objectives. To investigate activity gambogic lysinate (GAL) and mechanism. Methods. Inhibition cell was determined by MTT assay; intracellular ROS level detected staining cells with DCFH-DA; apoptosis flow cytometer mechanism GAL investigated Western blot. Results. inhibited MCF-7 IC 50 values 1.46 μ mol/L comparable (IC , 1.16 mol/L)....
KNDC1 (kinase noncatalytic C-lobe domain containing 1), a brain-specific Ras guanine nucleotide exchange factor, controls the negative regulation of neuronal dendrite growth. However, effect on cellular senescence remains to be elucidated. The present study investigated impact knockdown human endothelial cell and mechanisms underlying this effect. Human umbilical vein cells (HUVECs) cultured in vitro were used as model biological aging. Senescence‑associated β-galactosidase staining was...
Spontaneous type 2 diabetes mellitus (T2DM) macaques are valuable resources for our understanding the pathological mechanism of T2DM. Based on one month’s fasting blood glucose survey, we identified seven spontaneous T2DM and five impaired regulation (IGR) from 1408 captive individuals. FPG, HbA1c, FPI IR values were significant higher in IGR than controls. 16S rRNA sequencing fecal microbes showed significantly greater abundance Oribacterium, bacteria inhibiting production secondary bile...
Rhein lysinate (RHL), easily dissolved in water, is one of the anthraquinones, and has been shown to have anti-tumor activity different human cancer cell lines. In present study, we observed that RHL could cause vacuolar degeneration HeLa cells, which was not umbilical vein endothelial cells (HUVECs) other lines (SKOV-3 SK-BR-3). Therefore, purpose this study investigate effect rhein on cervix cells. The results indicated induce S-phase arrest (higher than 80 μM) also induced G2/M-phase a...
This study mainly explores how miR-183-5p pertains to breast cancer (BC) development. Functional assays were employed test impacts of in this cancer. Targeting between RGS2 and was examined with dual-luciferase assay, their interaction progression further unraveled. level noticeably high tissue/cells. Overexpressing could remarkably deteriorate progression. The regulatory gene levels markedly low BC, two genes we researched negatively correlated. It uncovered by rescue assay that...
In previous studies we observed that rhein lysinate (RHL), a salt of and lysine is easily dissolved in water, inhibited the growth tumor cells breast cancer, ovarian hepatocellular carcinoma cervical cancer. The present study aimed to investigate effects RHL on H460 A549 non-small cell lung cancer (NSCLC) using combination Taxol. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used determine inhibition effect drugs cells. Cell apoptosis analyzed by flow...
Overexpression of Sonic hedgehog (Shh) is associated with progression several cancers. The expression Shh in non-small cell lung cancer (NSCLC) has been reported inconsistent results. Lung adenocarcinoma (LAC) and squamous carcinoma (LSCC) are two major subtypes NSCLC, which have different genetic genotypes clinical therapeutic options. specimen patients NSCLC yet to be comprehensively determined according histological subtypes. level was 167 (56 LAC 111 LSCC patients) by immunohistochemical...
The current study aims to explore the changes of serum miR-587 level in patients with metabolic syndrome (MS) and analyze its clinical diagnostic value.The levels 50 hyperglycemia, hyperlipidemia, healthy controls were detected by RT-qPCR. value was ROC analy-sis correlation analysis MS. Dual luciferase reporter assay carried out determine possible target gene miR-587.The results RT-qPCR showed that relative content hyperglyce-mia hyperlipidemia 0.45 ± 0.30, 0.41 compared 1 0.87 people. area...
The current study aims to evaluate whether peripheral blood miR-324-5p could be used differentiate patients with metabolic disorders and healthy controls. Our data showed that levels were elevated in the of hyperglycemia or hyperlipidemia. In addition, expression was enhanced liver db/db mice. Further indicated overexpression reduced activation AKT/GSK pathway increased lipid accumulation, while inhibition activated decreased accumulation. A dual luciferase assay revealed Rho-associated...
The current study mainly evaluated whether peripheral blood miR-139 could be used as a biomarker to screen patients with metabolic disorders.The was collected hyperglycemia and high triglycerides (TG) combined total cholesterol (TC) well healthy control. Real time PCR carried out determine the relative level in disorders individuals. Receiver operating characteristic curve (ROC) analysis Spearman's correlation coefficient were evaluate possible application of potential for disorders. Dual...
Abstract Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and third leading cause of cancer-related deaths. Patient derived xenografts (PDX), generated by direct engraftment tumor samples from patients into immunocompromised mice can be used as an in vivo platform that provides opportunity to test proposed personalized medicine strategies. In this study, comprehensive molecular characterizations 28 Chinese HCC PDX models were done both whole genome sequencing (WGS)...
Abstract Patient-derived-xenograft (PDX) models have been widely used in the biomedical research. They recapitulate genomic diversity/heterogeneity of patient tumors, maintain a lot characteristics original tumors and showed much better correlation with clinical outcome comparing to traditional cell line derived xenograft models. However, relatively low throughput time-consuming nature PDX studies prevent them from being large scale screening or mechanistic studies. To address this,...