A5037963541- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Stress Responses and Cortisol
- COVID-19 Pandemic Impacts
- Neuroinflammation and Neurodegeneration Mechanisms
- Urinary Tract Infections Management
- African studies and sociopolitical issues
- Veterinary medicine and infectious diseases
- Enterobacteriaceae and Cronobacter Research
- Tryptophan and brain disorders
- Antibiotic Use and Resistance
National and Kapodistrian University of Athens
2015-2019
University General Hospital Attikon
2019
University of Thessaly
2013-2017
University of Antwerp
2015
We compared six colistin susceptibility testing (ST) methods on 61 carbapenem-nonsusceptible Klebsiella pneumoniae (n = 41) and Acinetobacter baumannii 20) clinical isolates with provisionally elevated MICs by routine ST. Colistin were determined broth microdilution (BMD), BMD 0.002% polysorbate 80 (P80) (BMD-P80), agar dilution (AD), Etest, Vitek2, MIC test strip (MTS). was used as the reference method for comparison. The EUCAST-recommended susceptible resistant breakpoints of ≤2 >2 μg/ml,...
Two colistin-susceptible/colistin-resistant (Col(s)/Col(r)) pairs of Acinetobacter baumannii strains assigned to international clone 2, which is prevalent worldwide, were sequentially recovered from two patients after prolonged colistin administration. Compared with the respective Col(s) isolates (Ab248 and Ab299, both having a MIC 0.5 μg/ml), Col(r) (Ab249 Ab347, MICs 128 32 μg/ml, respectively) significantly overexpressed pmrCAB genes, had single-amino-acid shifts in PmrB protein,...
Single-locus blaOXA-51-like sequence-based typing (SBT) was evaluated for its ability to determine correctly sequence types (STs) in Acinetobacter baumannii clinical isolates, comparison with the Pasteur's multilocus (MLST) reference method and 3-locus (3-LST). The comparative study performed 585 multidrug-resistant (MDR) A. isolates recovered from 21 hospitals located throughout Greece, Italy, Lebanon, Turkey. belonged nine clonal complexes (CCs) that correspond 12 distinct one singleton...
Colistin is commonly needed for the treatment of infections due to carbapenem-resistant Acinetobacter baumannii (CRAB) and determination its in vitro activity obviously important. However, accurate routine antimicrobial susceptibility testing (AST) colistin still challenging. The only acceptable method AST broth microdilution (BMD); disc gradient diffusion assays are inappropriate performance semi-automated systems has not been validated.In present study, two used were evaluated contemporary...
In two pairs of clinical colistin-susceptible/colistin-resistant (Cst(s)/Cst(r)) Acinetobacter baumannii strains, the Cst(r) strains showed significantly decreased biofilm formation in static and dynamic assays (P < 0.001) lower relative fitness 0.05) compared with those Cst(s) counterparts. The whole-genome sequencing comparison strain identified a mutation converting stop codon to lysine (*241K) LpsB (involved lipopolysaccharide [LPS] synthesis) one frameshift CarO loss 47,969-bp element...
Abstract Objectives Dependence on linezolid was recently described as significant growth acceleration of linezolid-resistant Staphylococcus epidermidis (LRSE) isolates upon exposure. We investigated the possible contribution dependence to LRSE dissemination in Greece. Methods Linezolid resistance rates were estimated six tertiary hospitals located throughout Greece between 2011 and 2013. Sixty-three randomly selected recovered these during this period studied. Growth curve analysis conducted...
Indoleamine 2,3-dioxygenase (IDO) suppresses adaptive immunity. It is known that IDO induces T-cell differentiation to regulatory T-cells (Treg) through tryptophan depletion and/or kynurenine pathway products. CD4+ effector require distinct metabolic programs in order support their function as compared Treg cells. Furthermore, glucose metabolism also affect B-cell survival and function. The effect of on lymphocytes was evaluated by using its inhibitor 1-methyl-DL-tryptophan (1-MT).Ten...
In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution resistance among clinical isolates A. baumannii group obtained from nine tertiary throughout Greece over 6 years (2010–2015). Identification and antimicrobial susceptibility testing were performed using Vitek 2 or Microscan walkaway automated systems. Between 2010 2015, to ampicillin/sulbactam increased 46.2 88.2 % (P=0.021),...
Linezolid-resistant Staphylococcus epidermidis (LRSE) and linezolid-dependent ST22 strains have been shown to predominate in tertiary care facilities all over Greece. We report herein the dissemination of but also ST2, ST5 ST168 LRSE clones four unrelated German hospitals.Fourteen clinical isolates recovered during 2012-14 from five distantly located hospitals were tested by for MIC determination broth microdilution Etest, PCR/sequencing cfr mutations 23S rRNA, rplC, rplD rplV genes, MLST,...