A5003141603- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Inhalation and Respiratory Drug Delivery
- Airway Management and Intubation Techniques
- Cardiac Arrest and Resuscitation
- Child and Adolescent Psychosocial and Emotional Development
- Asthma and respiratory diseases
- Air Quality and Health Impacts
- Pleural and Pulmonary Diseases
- Neuroscience of respiration and sleep
- Aquatic Ecosystems and Phytoplankton Dynamics
- Mental Health Research Topics
- Mechanical Circulatory Support Devices
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Heart Rate Variability and Autonomic Control
- Psychosomatic Disorders and Their Treatments
- Marine Toxins and Detection Methods
- Occupational and environmental lung diseases
- Occupational exposure and asthma
- Intensive Care Unit Cognitive Disorders
- Non-Invasive Vital Sign Monitoring
- Advanced Sensor and Energy Harvesting Materials
- Respiratory and Cough-Related Research
Petronas (Malaysia)
2024-2025
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2008-2022
Universidade Federal do Rio de Janeiro
2013-2022
Czech Academy of Sciences, Institute of Biophysics
2001-2022
Instituto de Biofísica Carlos Chagas Filho
2000-2020
Fundação Carlos Chagas
1987-2016
Universidade de São Paulo
1989-2009
University of Insubria
2009
Fundação de Amparo à Pesquisa do Estado de São Paulo
2009
Ospedale di Cattinara
2005-2009
In normal lungs, local changes in pleural pressure (P(pl)) are generalized over the whole surface. However, a patient with injured we observed (using electrical impedance tomography) pendelluft phenomenon (movement of air within lung from nondependent to dependent regions without change tidal volume) that was caused by spontaneous breathing during mechanical ventilation.To test hypotheses lungs negative P(pl) generated diaphragm contraction has localized effects (in regions) not uniformly...
Air pollution is associated with morbidity and mortality induced by respiratory diseases. However, the mechanisms therein involved are not yet fully clarified. Thus, we tested hypothesis that a single acute exposure to low doses of fine particulate matter (PM2.5) may induce functional histological lung changes unchain inflammatory oxidative stress processes. PM2.5 was collected from urban area São Paulo city during 24 h underwent analysis for elements polycyclic aromatic hydrocarbon...
To test whether pulmonary and extrapulmonary acute lung injury (ALI) of identical mechanical compromise would express diverse morphological patterns immunological pathways. For this purpose, a model (p) (exp) ALI with similar functional changes was developed morphology (light electron microscopy), cytokines levels, neutrophilic infiltration in the bronchoalveolar lavage fluid (BALF), elastic collagen fiber content alveolar septa, neutrophil apoptosis parenchyma were analyzed. BALB/c mice...
During bronchoscopy hypoxemia is commonly found and oxygen supply can be delivered by interfaces fed with high gas flows. Recently, the high-flow nasal cannula (HFNC) has been introduced for therapy in adults, but they have not used so far during adults. Forty-five patients were randomly assigned to 3 groups receiving oxygen: 40 L/min through a Venturi mask (V40, N = 15), (N40, 60 (N60, 15) bronchoscopy. Gas exchange circulatory variables sampled before (FiO(2) 0.21), at end of 0.5),...
This study was undertaken to test whether there is structural remodeling of lung parenchyma that could lead tissue mechanical changes at an early phase varying degrees acute injury (ALI). Tissue resistance (R), dynamic elastance (E), and hysteresivity ( η ) were analyzed during sinusoidal oscillations rat parenchymal strips 24 h after intraperitoneal injection saline (C) or paraquat (P [10, 15, 25, 30 mg/kg]). These also stained in order quantify the amount collagen three types elastic...
The effects of inspiratory flow rate and inflation volume on pulmonary mechanics were investigated in six anesthetized-paralyzed cats ventilated by constant-flow inflation. Pulmonary assessed using the technique rapid airway occlusion during which allows measurement intrinsic resistance (RLmin) overall "pulmonary resistance" (RLmax), includes additional pressure losses due to time constant inequalities within lung and/or stress adaptation. We observed that, at fixed volume, 1) RLmin fitted...
In six spontaneously breathing anesthetized subjects [halothane approximately 1 maximum anesthetic concentration (MAC), 70% N2O-30% O2], we measured flow (V), volume and tracheal pressure (Ptr). With airway occluded at end-inspiration tidal (VT), Ptr when the relaxed respiratory muscles. Dividing by VT, total system elastance (Ers) was obtained. subject still relaxed, occlusion released to obtain V-V relationship during ensuing expiration. Under these conditions, expiratory driving is V X...
In six spontaneously breathing anesthetized cats (pentobarbital sodium, 35 mg/kg ip) airflow, changes in lung volume and tracheal pressure were measured. The airways occluded at end inspiration (VT). During the ensuing period of apnea (Breuer-Hering inflation reflex), animal relaxed respiratory muscles passive compliance system (Crs) was computed by dividing VT pressure. While still relaxed, reopened, during expiration volume-flow relationship linear, slope representing time constant system:...
The aim of this study is to test the hypothesis that recruitment maneuvers (RMs) might act differently in models pulmonary (p) and extrapulmonary (exp) acute lung injury (ALI) with similar transpulmonary pressure changes.Prospective, randomized, controlled experimental study.University research laboratory.Wistar rats were randomly divided into four groups. In control groups, sterile saline solution was intratracheally (0.1 mL, Cp) or intraperitoneally (1 Cexp) injected, whereas ALI animals...
Eugenol, a methoxyphenol component of clove oil, suppresses cyclooxygenase-2 expression, while eugenol dimers prevent nuclear factor-kappaB (NF-kappaB) activation and inflammatory cytokine expression in lipopolysaccharide-stimulated macrophages. Our aim was to examine the vivo anti-inflammatory effects eugenol. BALB/c mice were divided into four groups. Mice received saline [0.05 ml intratracheally (it), control (Ctrl) (Eug) groups] or Escherichia coli LPS (10 microg it, LPSEug groups)....
To test the effects of positive expiratory pressure on leakage fluid around cuffs different tracheal tubes, in mechanically ventilated patients and a benchtop model.Randomized clinical trial experimental vitro study.Intensive care unit university hospital.Forty recovering intensive were volume-controlled mode. Twenty randomly intubated with Hi-Lo tubes (HL group), whereas remaining 20 subjects SealGuard (SG group).Immediately after intubation cuff inflation 30 cm H2O, Evans blue was applied...
We investigated the role of Fas ligand in murine silicosis. Wild-type mice instilled with silica developed severe pulmonary inflammation, local production tumor necrosis factor (TNF)-α, and interstitial neutrophil macrophage infiltration lungs. Strikingly, ligand–deficient generalized lymphoproliferative disease mutant (gld) did not develop The gld had markedly reduced extravasation into bronchoalveolar space, show increased TNF-α production, nor inflammation. Bone marrow chimeras adoptive...
This study tested the hypotheses that chronic allergic inflammation induces not only bronchial but also lung parenchyma remodeling, and these histologic changes are associated with concurrent in respiratory mechanics. For this purpose, airway remodeling were evaluated by quantitative analysis of collagen elastin, immunohistochemistry (smooth-muscle actin expression, eosinophil, dendritic cell densities), electron microscopy. In vivo (airway resistance, viscoelastic pressure, static...
In vivo (lung resistive and viscoelastic pressures static elastance) in vitro (tissue resistance, elastance, hysteresivity) respiratory mechanics were analyzed 1 30 days after saline (control) or paraquat (P [10 25 mg/kg intraperitoneally]) injection rats. Additionally, P10 P25 treated with methylprednisolone (2 intravenously) at 6 hours acute lung injury (ALI) induction. Collagen elastic fibers quantified. Lung elastance higher than the control. Tissue resistance augmented from control to...
The aim of this study is to test the hypothesis that early changes in lung mechanics and amount type III collagen fiber do not predict evolution parenchyma remodeling pulmonary extrapulmonary acute injury (ALI). For purpose, we analyzed time course murine models ALI with similar degrees mechanical compromise at phase ALI. Lung histology (light electron microscopy), elastic fibers alveolar septa, expression matrix metalloproteinase-9, parameters (lung-resistive viscoelastic pressures, static...
This study tests the hypotheses that a recruitment maneuver per se yields and/or intensifies lung mechanical stress. Recruitment was applied to model of paraquat-induced acute injury (ALI) and healthy rats with (ATEL) or without (CTRL) previous atelectasis. done by using 40-cmH 2 O continuous positive airway pressure for 40 s. Rats were, then, ventilated 1 h at zero end-expiratory (ZEEP) (PEEP; 5 cmH O). Atelectasis generated inflating sphygmomanometer around thorax. Additional groups did...