Intestinal barrier function is important for preserving health, as a compromised allows antigen entry and can induce inflammatory diseases. Probiotic bacteria play role in enhancing intestinal function; however, the mechanisms are not fully understood. Existing studies have focused on ability of probiotics to prevent alterations tight junctions disease models, been restricted few junction bridging proteins. No previously investigated effect probiotic healthy epithelial cell genes involved...

The traditional Mediterranean diet is thought to represent a healthy lifestyle; especially given the incidence of several cancers including colorectal cancer lower in countries compared Northern Europe. Olive oil, central component diet, believed beneficially affect numerous biological processes. We used phenols extracted from virgin olive oil on series vitro systems that model important stages colon carcinogenesis. effect extract DNA damage induced by hydrogen peroxide was measured HT29...

Background: There is a probable association between consumption of fruit and vegetables reduced risk cancer, particularly cancer the digestive tract.This anti-cancer activity has been attributed in part to anti-oxidants present these foods.Raspberries particular are rich source anti-oxidant compounds, such as polyphenols, anthocyanins ellagitannins.Methods: A "colon-available" raspberry extract (CARE) was prepared that contained phytochemicals surviving digestion procedure mimicked...

Abstract In recent years, the use of anthraquinone laxatives, in particular senna, has been associated with damage to intestinal epithelial layer and an increased risk developing colorectal cancer. this study, we evaluated cytotoxicity rhein, active metabolite on human colon adenocarcinoma cells (Caco‐2) its effect cell proliferation. Cytotoxicity studies were performed using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), neutral red (NR) trans‐epithelial electrical...

Abstract Abstract: Although it is known to be a rich source of the putative anti-cancer chemicals isothiocyanates, watercress has not been extensively studied for its cancer preventing properties. The aim this study was investigate potential chemoprotective effects crude extract toward three important stages in carcinogenic process, namely initiation, proliferation, and metastasis (invasion) using established vitro models. HT29 cells were used protective on DNA damage cell cycle. genotoxic...

The soybean-derived protease inhibitor, Bowman-Birk inhibitor (BBI), is currently showing great promise as a novel cancer chemopreventive agent. In contrast to the wealth of research conducted on this compound, anticancer effects inhibitors isolated from other leguminous sources have received limited attention. current study, 7 concentrates (PICs) were various (including soybean) and characterized. PICs proliferation breast prostate cells investigated in vitro. Chickpea PIC significantly...

Inflammatory bowel disease (IBD) is a chronic relapsing disease. Genetic predisposition to the reduces an individual’s capacity respond appropriately environmental challenges in intestine leading inappropriate inflammation. IBD patients often modify their diet mitigate or reduce severity of Turmeric (Curcuma longa L., Zingiberaceae) has historically been used Chinese, Hindu, and Ayurvedic medicine over several centuries treat inflammatory disorders. To understand how turmeric may influence...

The decreased cancer risk associated with consumption of olive oil may be due to the presence phenolics which can modulate pathways including apoptosis and invasion that are relevant carcinogenesis. We have previously shown a virgin extract (OVP) inhibited HT115 colon cells in vitro. In current study we assessed vitro effects OVP (25 μg mL−1) on cell migration, spreading integrin expression. Furthermore, anti-metastatic activity – at dose equivalent 25 mg per kg day for 2, 8 or 10 weeks was...

The ability to predictably engineer the composition of bowel microbial communities (microbiota) using dietary components is important because reported associations altered microbiota with medical conditions. In a synecological study, weanling conventional Sprague-Dawley rats (21 days old) were fed basal diet (BD) or supplemented resistant starch (RS) at 5%, 2.5%, 1.25% for 28 days. Pyrosequencing 16S rRNA genes and temporal temperature gradient electrophoresis (TTGE) profiles in colonic...

The mammalian lignan, enterolactone, has been shown to reduce the proliferation of earlier stages prostate cancer at physiological concentrations in vitro. However, efficacy later disease occurs difficult achieve through dietary modification. We have therefore investigated what concentration(s) enterolactone can restrict multiple using an vitro model system disease. determined that 20 μM significantly restricted mid and late stage models These effects were strongly associated with changes...

Ecological data suggest a long-term diet high in plant material rich biologically active compounds, such as the lignans, can significantly influence development of prostate cancer over lifetime an individual. The capacity pure mammalian lignan, enterolactone (ENL), to proliferation LNCaP human cell line was investigated function density, metabolic activity, expression and secretion specific antigen (PSA), cycle profile, genes involved progression cancer. Treatment with subcytotoxic...

Scope There is evidence that a mammalian lignan, enterolactone ( ENL ), decreases the proliferation rate of prostate cancer cells, although previous studies have used concentrations difficult to achieve through dietary modification. We therefore investigated anti‐proliferative effects in an vitro model tumourigenesis at reported occur range male populations. Methods and results The 0.1 1 μM on three markers viability (metabolic activity, growth kinetics, cell cycle progression) were assessed...

Se is an essential micronutrient for human health, and fluctuations in levels the potential cellular dysfunction associated with it may increase risk disease. Although has been shown to influence several biological pathways important little known about effect of on expression microRNA (miRNA) molecules regulating these pathways. To explore role Se-sensitive miRNA linked colon cancer, we profiled 800 CaCo-2 adenocarcinoma cell line response a low-Se (72 h at <40 nm) environment using nCounter...

Lactobacillus fermentum is found in fermented foods and thought to be harmless. In vivo clinical studies indicate that some L. strains have beneficial properties, particularly for gastrointestinal health. However, AGR1487 decreases trans-epithelial electrical resistance (TEER), a measure of intestinal barrier integrity. The hypothesis was the expression cell tight junction genes proteins, thereby reducing Transcriptomic proteomic analyses Caco-2 cells (model human epithelial cells) treated...

The use of deer velvet antler (DVA) as a potent traditional medicine ingredient goes back for over 2000 years in Asia. Increasingly, though, DVA is being included high protein functional food convenient, ready to consume products Korea and China. As such, it potential source endogenous bioactive peptides 'cryptides', i.e. enzymatically released by proteases, processing and/or gastrointestinal digestion. Fermentation an example step known release from proteins. In this study, we aimed...

Abstract Type 2 diabetes mellitus is a major health concern worldwide; however, the molecular mechanism underlying its development poorly understood. The hormone amylin postulated to be involved, as human forms amyloid in pancreases of diabetic patients, and oligomers have been shown cytotoxic β-cells. As rodent non-amyloidogenic, mice expressing developed investigate this hypothesis. However, it not possible differentiate effects overexpression from β-cell loss these models. We transgenic...

Intestinal health problems are common and therefore smart foods that enhance intestinal wellness or reduce/ prevent these in demand. In order to develop new for take advantage of the developing knowledge regarding interactions between humans beyond simply nutrition, vitro vivo models disease required. used screen large numbers foods, identify candidates with desirable properties, hypotheses about mechanisms action beneficial food components. These candidate can then be tested efficacy...