A5100417288- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Trace Elements in Health
- Cardiac Ischemia and Reperfusion
- Alcohol Consumption and Health Effects
- Organ Donation and Transplantation
- Adipose Tissue and Metabolism
- Renal Transplantation Outcomes and Treatments
- Pancreatitis Pathology and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Kruppel-like factors research
- Heme Oxygenase-1 and Carbon Monoxide
- Genomics, phytochemicals, and oxidative stress
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- HER2/EGFR in Cancer Research
- Alzheimer's disease research and treatments
- Acute Kidney Injury Research
- Sirtuins and Resveratrol in Medicine
- Liver physiology and pathology
- Drug Transport and Resistance Mechanisms
Wenzhou Medical University
2025
First Affiliated Hospital of Wenzhou Medical University
2025
Zhongnan Hospital of Wuhan University
2015-2024
Wuhan University
2015-2024
University of Pennsylvania
2019-2024
University of Electronic Science and Technology of China
2024
Children's Hospital of Philadelphia
2019-2024
Io Therapeutics (United States)
2024
Huazhong University of Science and Technology
2015-2018
Northwest A&F University
2012
Conducting polymers have emerged as frontrunners to be alternatives for nerve regeneration, showing a possibility of the application polyaniline (PANI) guidance conduit. In present work, cellulose hydrogel was used template in situ synthesize PANI via limited interfacial polymerization method, leading one conductive side polymer. sub-micrometer dendritic particles with mean diameter ∼300 nm consisting nanofibers and nanoparticles were uniformly assembled into matrix. The hydrophobic...
Abstract Accumulating evidence suggests that caspase-3 plays critical roles beyond apoptosis, serving pro-survival functions in malignant transformation and tumorigenesis. However, the mechanism of non-apoptotic action oncogenic remains unclear. In present study, we show is consistently activated induced by exogenous expression cocktail (c-Myc, p53DD, Oct-4, H-Ras) vitro as well mouse mammary tumor virus-polyomavirus middle T antigen (MMTV-PyMT) model breast cancer. Genetic ablation...
Renal transplantation is currently the most effective treatment for end-stage renal disease. However, chronic antibody-mediated rejection (cABMR) remains a serious obstacle long-term survival of patients with and problem to be solved. At present, role mechanism underlying immune factors such as T- B- cell subsets in cABMR after remain unclear. In this study, single-cell RNA sequencing (scRNA-seq) peripheral blood monocytes (PBMCs) from control subjects was performed define transcriptomic...
Abstract CDK5 activation promotes ischemic neuronal death in stroke, with the recognized mechanism being calpain-dependent p35 cleavage to p25. Here we reported that CDK5-Tyr15 phosphorylation by zinc induced brain injury. and were observed hippocampus of rats had been subjected middle cerebral artery occlusion, both which reversed pretreatment chelator; while calpain ischemia not reversed. Zinc incubation resulted activation, without increasing cultured cells. Site mutation experiment...
A previous study has demonstrated a progression in the nerve regeneration by polyaniline/cellulose (PANI/RC), although underlying mechanism was not elucidated. In present study, regenerated nerves were investigated, using histological techniques, functional assays and western blot analysis. The triceps surae muscle weight ratio percentages of sham, cellulose (RC) PANI/RC groups 38.88±4.76 76.32±7.11%, respectively. thickness myelin sheath for aforementioned as follows: 1.2±0.27; 0.49±0.21...
There is an urgent need to improve the quality of donor organs obtained after cardiac death. In present study, we examined potential mechanisms through which A20 protects renal cells against ischemia/reperfusion injury (IRI) following either hypothermic machine perfusion (HMP) or static cold storage (CS) kidneys in a rabbit model. The expression markers apoptosis, necroptosis and inflammation frozen kidney tissues were detected by western blot analysis, RT-qPCR ELISA. Compared with CS group,...
Hepatic ischemia/reperfusion injury (HIRI) is a complex pathophysiological process that often leads to poor clinical prognosis. Clinically, the effective means alleviate HIRI are limited. The aim of present study was investigate whether Alda‑1, an activator mitochondrial aldehyde dehydrogenase 2 (ALDH2), had protective effect on and mechanisms underlying this effect. Sprague‑Dawley rats were treated with Alda‑1 or Daidzin, ALDH2 inhibitor, 30 min before partial (70%) warm liver ischemia...
Hypothermic machine perfusion (MP) can reduce graft's injury after kidney transplantation; however, the mechanism has not been elucidated. In past decade, many studies showed that aldehyde dehydrogenase 2 (ALDH2) is a protease which inhibit cell apoptosis. Therefore, this study aims to explore whether ALDH2 takes part in reducing organ damage MP. Eighteen healthy male New Zealand rabbits (12 weeks old, weight 3.0 ± 0.3 kg) were randomly divided into three groups: normal group, MP and cold...
Hypothermic oxygenated perfusion (HOPE) is a relatively new dynamic preservation procedure that has not been widely implemented in liver transplantation despite its advantages. Improved graft protection one such advantage offered by HOPE and attributed to multiple mechanisms, of which may be the modulation thioredoxin-interacting protein (TXNIP)/NOD-like receptor 3 (NLRP3) inflammasome pathway. The TXNIP/NLRP3 pathway plays critical role sterile inflammation under oxidative stress as result...
Hypothermic machine perfusion (HMP) has been known as an efficient way to improve kidney graft function, but the underlying mechanisms remain unclear. Here, we adopt a rabbit reperfusion mode investigate upstream of end-ischemic HMP kidneys from donors after cardiac death (DCD), with static cold storage (CS) control. Eighteen New Zealand healthy male rabbits (12 weeks old, weight 3.0 ± 0.2 kg) were randomly divided into three groups: group, CS and Normal group (n = 6). The left underwent...
This study aimed to explore the potential mechanisms of hypothermic machine perfusion (HMP)-a more efficient way preserve kidneys from donors after cardiac death than static cold storage (CS), then provide basis for further improving donor quality. Twelve healthy male New Zealand rabbits (12 weeks old, weighing 3.0 ± 0.3 kg) were randomly divided into two groups: HMP group and CS (n = 6). Rabbits' left kidney was subjected 35 min warm ischemic time by clamping renal pedicle 1 h reperfusion....
Severe hepatic ischemia reperfusion injury (IRI) can result in poor short- and long-term graft outcome after transplantation. The way to improve the viability of livers from donors circulatory death (DCD) is currently limited. aim present study was explore protective effect simvastatin on DCD investigate underlying mechanism.24 male rats randomly received or its vehicle. 30 min later, rat were exposed warm situ for min. Livers removed cold-stored UW solution 24 h, subsequently reperfused 60...
Hepatic ischemia/reperfusion injury (HIRI) serves a causative role in postoperative hepatocyte death; however, the mechanisms underlying HIRI remain unclear. Mitochondrial autophagy, with apoptosis, cell cycle distribution and DNA damage repair, may be regarded as regulatory factor post‑HIRI. Parkin, novel ubiquitin ligase, has been reported to increase mitochondrial autophagy decrease apoptosis. However, association between other factors is In present study, effects of Parkin on were...
Abstract The exact mechanism by which hypothermic machine perfusion ( HMP ) improves the graft quality in kidney transplantation of donation after cardiac death DCD remains unclear. aim this study was to investigate correlation between expression matrix metalloproteinase 9 MMP ‐9) and inflammatory reaction ischemia‐reperfusion I / R injury followed cold storage CS or model . N ew Z ealand white rabbit kidneys were subjected 35 min warm ischemia 1 h reperfusion, then preserved either...
Abstract The protective role of hypoxia‐inducible factor‐1 (HIF‐1) against liver ischemia‐reperfusion injury has been well proved. However its in donation and preservation from after cardiac death (DCD) is still unknown. objective this study was to test the hypothesis that pharmaceutical stabilization HIF‐1 DCD donors would result a better graft condition. Male SD rats (6 animals per group) were randomly given synthetic prolyl hydroxylase domain inhibitor FG‐4592 (Selleck, 6 mg/kg body...
Objective. Ischemia–reperfusion injury (IRI) is an important cause of delayed functional recovery after transplantation. This study aimed at investigating the molecular mechanism ALDH2 in a kidney ischemia–reperfusion model based on RNA-seq. Methods. We performed ALDH2-/- and WT mice evaluated function morphology using SCr, HE staining, TUNEL TEM. used RNA-seq to compare mRNA expression IR, then, we verified related pathways by PCR western blotting. In addition, activators inhibitors were...
BACKGROUND The recipient's pattern of hepatic veins (HVs) drainage into the inferior vena cava (IVC) (drainage pattern, for short) may influence outflow reconstruction and thus venous obstruction (HVOO) in piggyback liver transplantation (PBLT). However, no previous study has investigated this association. MATERIAL AND METHODS A retrospective analysis 202 PBLT (2000-2016) was conducted. Based on patterns, patients were divided Group (common trunk left middle HVs), B right C 3 HVs). Patients'...
Hypothermic machine perfusion (HMP) has been demonstrated to be a more effective method for preserving livers donated after circulatory death (DCD) than cold storage (CS); however, the underlying mechanisms remain unclear. The aim of present study was investigate protective effects HMP on rat DCD and possible role nuclear factor erythroid 2‑related 2 (Nrf2)/antioxidant response element (ARE) signaling pathway. A total 18 adult male rats were randomly divided into three groups: Control, CS...