A5082085969- Epigenetics and DNA Methylation
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- Glioma Diagnosis and Treatment
- Celiac Disease Research and Management
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Metabolism and Genetic Disorders
- Histone Deacetylase Inhibitors Research
- Nutritional Studies and Diet
- Diet and metabolism studies
- RNA modifications and cancer
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolites in Food Biotechnology
- Inflammatory Bowel Disease
- Adolescent and Pediatric Healthcare
- Birth, Development, and Health
- Lysosomal Storage Disorders Research
- Sexual Differentiation and Disorders
- Genetic Syndromes and Imprinting
- Probiotics and Fermented Foods
- Microscopic Colitis
- Sperm and Testicular Function
- Genetics and Neurodevelopmental Disorders
- Obesity, Physical Activity, Diet
University of Naples Federico II
2013-2024
Ceinge Biotecnologie Avanzate (Italy)
2020-2024
California Liver Research Institute
2024
Columbia University Irving Medical Center
2023
Federico II University Hospital
2013-2022
Azienda Socio Sanitaria Territoriale Santi Paolo e Carlo
2022
Ospedale San Carlo
2022
Institute for Experimental Endocrinology and Oncology
2017-2019
National Research Council
2017-2019
Proliferation and/or depletion of clusters specific bacteria regulate intestinal functions and may interfere with neuro-immune communication behavior in patients autism spectrum disorder (ASD). Consistently, qualitative quantitative alteration bacterial metabolites functionally affect ASD pathophysiology. Up to date, age-restricted cohort studies, that potentially help identify microbial signatures ASD, are lacking. We investigated the gut microbiota (GM) structure fecal short chain fatty...
Abstract Alterations of microbiota-gut-brain axis have been invoked in the pathogenesis autism spectrum disorders (ASD). Mouse models could represent an excellent tool to understand how gut dysbiosis and related alterations may contribute autistic phenotype. In this study we paralleled microbiota (GM) profiles, behavioral characteristics, intestinal integrity immunological features colon tissues BTBR T + tf/J (BTBR) inbred mice, a well established animal model ASD. Sex differences, up date...
A large number of studies have highlighted the important role gut microbiota in pathophysiology neurological disorders, suggesting that its manipulation might serve as a treatment strategy. We hypothesized participates absence seizure development and maintenance WAG/Rij rat model tested this hypothesis by evaluating potential intestinal alterations model, well measuring impact using fecal transplantation (FMT).Initially, composition histology rats (a well-recognized genetic epilepsy) were...
Periodontitis is one of the most common oral inflammatory diseases, and results in connective tissue degradation gradual tooth loss. It manifests with formation periodontal pockets, which anaerobic Gram‑negative bacteria proliferate rapidly. Consequently, alteration subgingival microbiota considered primary etiologic agent periodontitis. Previous studies have reported that smokers are at increased risk disease, both prevalence severity, indicating smoking a factor for onset progression...
The spatio-temporal regulation of genes involved in the synthesis and degradation D-serine D-aspartate such as serine racemase (SR), D-amino acid oxidase (DAO), G72 (DDO), play pivotal roles determining correct levels these acids human brain. Here we provide a comprehensive analysis mRNA expression DNA methylation status post-mortem samples from hippocampus, dorsolateral prefrontal cortex, cerebellum patients with schizophrenia non-psychiatric controls. was performed at an ultradeep level,...
The bidirectional microbiota-gut-brain axis has raised increasing interest over the past years in context of health and disease, but there is a lack information on molecular mechanisms underlying this connection. We hypothesized that change microbiota composition may affect brain epigenetics leading to long-lasting effects specific gene regulation. To test hypothesis, we used Zebrafish (Danio Rerio) as model system. As previously shown, treatment with high doses probiotics can modulate...
Abstract Accumulating evidence suggests that modifications of gut function and microbiota composition might play a pivotal role in the pathophysiology several cardiovascular diseases, including heart failure (HF). In this study we systematically analysed composition, intestinal barrier integrity, serum cytokines endotoxin levels C57BL/6 mice undergoing pressure overload by transverse aortic constriction (TAC) for 1 4 weeks. Compared to sham-operated animals, TAC induced prompt strong...
Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on accessibility DNA methylation regions critical for such as enhancers promoters. These epigenetic modifications required mammalian development essential establishment maintenance cellular identity. was once thought to be a permanent repressive mark, but systematic analyses in various genomic contexts have revealed more dynamic regulation than previously thought. In fact, both active...
Programmed epigenetic modifications occurring at early postnatal brain developmental stages may have a long-lasting impact on function and complex behavior throughout life. Notably, it is now emerging that several genes undergo perinatal changes in DNA methylation are associated with neuropsychiatric disorders. In this context, we envisaged during the period potentially drive essential regulating levels of critical neuromodulators such as D-serine D-aspartate. Dysfunction fine regulation...
Background: We have recently reported the downregulation of Metallophosphoesterase-domain-containing protein 2 (MPPED2) gene and its cognate long non-coding RNA, MPPED2-AS1, in papillary thyroid carcinomas. Functional studies supported a tumor suppressor role both these genes carcinogenesis. then decided to investigate their breast Methods: In order verify MPPED2 expression, 45 human carcinoma samples been investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Then, has...
DNA methylation is a heritable epigenetic mark that plays key role in regulating gene expression. Mathematical modeling has been extensively applied to unravel the regulatory mechanisms of this process. In study, we aimed investigate by performing high-depth analysis particular loci, and subsequent experimental results. particular, performed an in-deep profiling two genomic loci surrounding transcription start site D-Aspartate Oxidase D-Serine genes different samples (n = 51). We found...
Unresectable neuroendocrine neoplasms (NENs) often poorly respond to standard therapeutic approaches. Alkylating agents, in particular temozolomide, commonly used treat high-grade brain tumors including glioblastomas, have recently been tested advanced or metastatic NENs, where they showed promising response rates. In prediction of temozolomide is based on the assessment methylation status MGMT gene, as its product, O6-methylguanine-DNA methyltransferase, may counteract damaging effects...
The gut-brain axis involves several bidirectional pathway communications including microbiome, bacterial metabolites, neurotransmitters as well immune system and is perturbed both in brain gastrointestinal disorders. Consistently, microbiota-gut-brain has been found altered autism spectrum disorder (ASD). We reasoned that such alterations occurring ASD may impact on methylation signatures of human host fecal DNA (HFD) possibly the types cells shed stools from intestinal tract giving origin...
Recessive X-linked disorders may occasionally evolve in clinical manifestations of variable severity also female carriers. For some such diseases, the frequency symptoms' appearance during women's life be particularly relevant. This phenomenon has been largely attributed to potential skewness X-inactivation process leading phenotypes. Nonetheless, many cases, no correlation with unbalance was demonstrated. However, methods for analyzing have mainly limited Human Androgen Receptor methylation...
Abstract FXYD1 is a key protein controlling ion channel transport. exerts its function by regulating Na + /K -ATPase activity, mainly in brain and cardiac tissues. Alterations of the expression level cause diastolic dysfunction arrhythmias heart decreased neuronal dendritic tree spine formation brain. Moreover, FXYD1, target MeCP2, plays crucial role pathogenesis Rett syndrome, neurodevelopmental disorder. Thus, amount must be strictly controlled tissue specific manner and, likely, during...
Molecular differentiation between benign (follicular thyroid adenoma (FTA)) and malignant carcinoma (FTC)) neoplasms is challenging. Here, we explored the genome-wide DNA methylation profile of FTA (n.10) FTC (n.11) compared to normal (NT) (n.7) tissues. featured 3564 differentially methylated CpGs (DMCpG), most (84%) them hypermethylated, with respect controls. At principal component analysis (PCA), occupied an intermediate position tissue. A large fraction (n. 2385) FTC-associated DMCpG...
DNA methylation is a stable epigenetic modification, extremely polymorphic and driven by stochastic deterministic events. Most of the current techniques used to analyse methylated sequences identify cytosines (mCpGs) at single-nucleotide level compute average CpGs in population molecules. Stable epialleles, i.e. CpG strings with same sequence containing discrete linear succession phased methylated/non-methylated molecule, cannot be identified due heterogeneity 5'-3' ends Moreover, these are...