A5002933216- Leptospirosis research and findings
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Viral Infections and Vectors
- Advanced Nanomaterials in Catalysis
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Veterinary medicine and infectious diseases
- RNA Interference and Gene Delivery
- Cardiac Valve Diseases and Treatments
- Mycobacterium research and diagnosis
- Ferroptosis and cancer prognosis
- IL-33, ST2, and ILC Pathways
- Bacterial Infections and Vaccines
- Cell death mechanisms and regulation
- Mechanical Failure Analysis and Simulation
- Epigenetics and DNA Methylation
- Microbial Metabolism and Applications
- Gene expression and cancer classification
- Asthma and respiratory diseases
- Molecular Sensors and Ion Detection
- T-cell and B-cell Immunology
- Biomarkers in Disease Mechanisms
- Wnt/β-catenin signaling in development and cancer
Zhejiang University
2014-2025
Pharmacyclics (United States)
2008-2023
The University of Texas at Austin
2023
Children's Hospital of Zhejiang University
2022
First Affiliated Hospital Zhejiang University
2013-2019
Center for Innovation
2019
Indiana University School of Medicine
2017-2018
Indiana University – Purdue University Indianapolis
2017-2018
Huazhong Agricultural University
2017
Huazhong University of Science and Technology
2015
Leptospirosis is a global zoonotic disease. Transmission of Leptospira from animals to humans occurs through contact with water contaminated leptospire-containing urine infected animals. However, the molecular basis for invasiveness and transmission leptospirosis remains unknown. Activity interrogans strain Lai colA gene product (ColA) hydrolyze different collagenic substrates was determined by spectrophotometry. Expression secretion ColA during infection were detected reverse-transcription...
Background Leptospira interrogans is the major causative agent of leptospirosis. Phagocytosis plays important roles in innate immune responses to L. infection, and can evade killing phagocytes. However, little known about adaptation during this process. Methodology/Principal Findings To better understand interaction pathogenic immunity, we employed microarray comparative genomics analyzing macrophage-derived cells. During process, altered expressions many genes involved carbohydrate lipid...
Abstract Several water-solubilized versions of the zinc ionophore 1-hydroxypyridine-2-thione (ZnHPT), synthesized as part present study, have been found both to increase intracellular concentrations free and produce an antiproliferative activity in exponential phase A549 human lung cancer cultures. Gene expression profiles cultures treated with one these water-soluble ionophores, PCI-5002, reveal activation stress response pathways under control metal-responsive transcription factor 1...
To identify the major infiltrating phagocytes during leptospirosis and examine killing mechanism used by host to eliminate Leptospira interrogans.Major in Leptospira-infected C3H/HeJ mice were detected immunohistochemistry. Chemokines vascular endothelial cell adhesion molecules (VECAMs) of patients microarray Leptospira-phagocytosing -killing abilities human or mouse macrophages neutrophils, roles intracellular ROS, NO [Ca2+]i Leptospira-killing process evaluated confocal microscopy...
Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade organs after to disease aggravation, but mechanism of leptospiral excretion spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial epithelial cells fibroblasts caveolae/integrin-β1-PI3K/FAK-mediated...
Pathogenic Leptospira species, the causative agents of leptospirosis, have been shown to induce macrophage apoptosis through caspase-independent, mitochondrion-related inducing factor (AIF) and endonuclease G (EndoG), but signalling pathway leading AIF/EndoG-based remains unknown. Here we show that infection interrogans caused a rapid increase in reactive oxygen species (ROS), DNA damage, intranuclear foci 53BP1 phosphorylation H2AX (two DNAdamage indicators) wild-type p53-containing mouse...
Abstract Background Targeting oncogenic histone modification by deacetylase inhibitors (HDACis) demonstrates promising prospects in clinical cancer treatment, whereas a notable proportion of patients cannot benefit from HDACi therapy. This study aims to explore how influences the tumor microenvironment, order identify potential targets for reversing resistance therapies. Methods Macrophage infiltration was compared between HDACi-responding and HDACi-nonresponding patients. The impact HDACis...
Leptospirosis is a global zoonotic infectious disease caused by pathogenic Leptospira species. Leptospire-induced macrophage apoptosis through the Fas/FasL-caspase-8/3 pathway plays an important role in survival and proliferation of pathogen hosts. Although release mitochondrial apoptosis-inducing factor (AIF) endonuclease G (EndoG) leptospire-infected macrophages has been described, mechanisms linking caspase mitochondrion-related host-cell not determined. Here, we demonstrated that...
Treg cells play a crucial role in immune tolerance, but mechanisms that induce are poorly understood. We here have described eosinophils lamina propria (LP) displayed high aldehyde dehydrogenase (ALDH) activity, rate-limiting step during all-trans retinoic acid (ATRA) synthesis, and expressed TGF-β1 mRNA levels of ATRA. Co-incubation assay confirmed LP induced the differentiation naïve T into cells. Differentiation promoted by were inhibited blocked either or Peripheral blood (PB) did not...
Leptospira interrogans is the major causative agent of leptospirosis, an emerging, globally spreading zoonotic infectious disease. The pathogen induces macrophage apoptosis, but molecular basis and mechanism remain unknown. In present study, we found that L. caused apoptosis phagocytosis-inhibited macrophages, product LB047 gene (Lep-OMP047) was unique protein captured by mouse human Fas proteins. recombinant expressed Lep-OMP047 (rLep-OMP047) strongly bound proteins with equilibrium...
The identification of prognostic biomarkers for cancer patients is essential research. These days, DNA methylation has been proved to be associated with prognosis. However, there are few methods which identify the markers based on data systematically, especially considering interaction among sites. In this paper, we first evaluated stabilities microRNA, mRNA, and in prognosis cancer. After that, a rank-based method was applied construct network. network, nodes largest degrees (10% all nodes)...
BackgroudLeptospira interrogans is the major causative agent of leptospirosis, a worldwide zoonotic disease. Hemorrhage typical pathological feature leptospirosis. Binding von Willebrand factor (vWF) to platelet glycoprotein-Ibα (GPIbα) crucial step in initiation aggregation. The products L. vwa-I and vwa-II genes contain vWF-A domains, but their ability induce hemorrhage has not been determined.MethodsHuman (Hu)-platelet- Hu-GPIbα-binding abilities recombinant proteins expressed by strain...
Leptospirosis caused by Leptospira is a zoonotic disease of global importance but it considered as an emerging or re-emerging infectious in many areas the world. Until now, mechanisms about pathogenesis and transmission remains poorly understood. As eukaryotic prokaryotic proteins can be denatured adverse environments chaperone-protease/peptidase complexes degrade these harmful proteins, we speculate that infection may also cause leptospiral protein denaturation, HslU HslV L. interrogans...
Nontypeable Haemophilus influenzae (NTHi) is one of the most common etiologies acute otitis media, rhinosinusitis, and pneumonia. Outer membrane proteins (OMPs) are main focus in new vaccine development against NTHi, as H. type b (Hib) does not cover noncapsulated NTHi. The OMPs P6 protein D promising candidate antigens for an NTHi vaccine, low antibody levels them serum may be correlated with infection caused by In current study, we measured titers P6, D, their T- B-cell combined peptide...
Abstract Quorum sensing (QS) is a mechanism that allows bacteria to regulate various physiological and biochemical functions by secreting, responding signaling molecules called autoinducers (AIs). In Vibrio species, QS plays crucial role in modulating different biological characteristics. can influence the formation of biofilms, which are communities encased protective matrix. It also controls flagella motility, ensuring spp. move efficiently response environmental cues. Additionally,...
Abstract Objective This investigation aimed to delineate the clinical manifestations associated with high‐altitude pulmonary edema (HAPE) and acute mountain sickness (AMS) in pediatric populations find risk factors of HAPE. Methods We conducted a retrospective analysis data from children under 18 years diagnosed HAPE AMS at an average altitude 3000 m. The between these two groups were compared. Results study encompassed 74 patients, 27 47 presentations included classic (55.3%), reentry...
Background Pathogenic species of Leptospira cause leptospirosis, a global zoonotic disease. Our previous work showed that leptospires survive and replicate in human macrophages but are killed murine macrophages. However, the mechanism responsible for different intracellular fates within hosts remains unclear. Results The present study demonstrates infection with interrogans caused significant up-regulation reactive oxygen (ROS) superoxide J774A.1 cells did so to lesser extent THP-1 cells....
Leptospira spp. comprise both pathogenic and free-living saprophytic species. Little is known about the environmental adaptation survival mechanisms of Leptospira. Alternative sigma factor, σ
Leptospirosis is a worldwide zoonosis caused by spirochetes from the genus Leptospira. In present study, visual and rapid method for detecting Leptospira interrogans was developed based on multiple cross-displacement amplification (MCDA) nanoparticle-based lateral flow biosensor (LFB). A set of 10 primers specifically designed to recognize regions lipL 41 gene L. interrogans. The MCDA reaction optimized at 64°C only 40 min, products were directly applied biosensor. entire process, including...