Neuronal pyroptosis and neuroinflammation with excess microglial activation are widely involved in the early pathological process of ischemic stroke. Repetitive transcranial magnetic stimulation (rTMS), as a non-invasive neuromodulatory technique, has recently been reported to be anti-inflammatory regulate function. However, few studies have elucidated role mechanism rTMS underlying regulating neuronal polarization.We evaluated motor function middle cerebral artery occlusion/reperfusion...

Abstract Metabolic reprogramming refers to the ability of a cell alter its metabolism in response different stimuli and forms pressure. It helps cells resist external stress provides them with new functions. Skin wound healing involves metabolic nutrients, such as glucose, lipids, amino acids, which play vital roles proliferation, differentiation, migration multiple types. During glucose process wounds, transporters key enzymes cause elevated metabolite levels. Glucose-mediated oxidative...

Abstract Background Adipose mesenchymal stem cell-derived exosomes (ADSC-Exos) have great potential in the field of tissue repair and regenerative medicine, particularly cases refractory diabetic wounds. Interestingly, autophagy plays a role wound healing, recent research has demonstrated that are closely associated with intracellular biogenesis molecular signaling mechanisms. Therefore, this study aimed to investigate whether ADSC-Exos promote wounds by regulating provide new method...

Nanoparticle-based therapeutics represent potential strategies for treating atherosclerosis; however, the complex plaque microenvironment poses a barrier nanoparticles to target dysfunctional cells. Here, we report reactive oxygen species (ROS)-responsive and size-reducible nanoassemblies, formed by multivalent host-guest interactions between β-cyclodextrins (β-CD)-anchored discoidal recombinant high-density lipoprotein (NP3ST) hyaluronic acid-ferrocene (HA-Fc) conjugates. The HA-Fc/NP3ST...

Gpbar1 (TGR5), a membrane-bound bile acid receptor, is well-known for its roles in regulation of energy homeostasis and glucose metabolism. Here, we show that mice lacking TGR5 were much more susceptible to lipopolysaccharide (LPS)-induced acute gastric inflammation than wild-type (WT) negative regulator through antagonizing NF-κB signaling pathway. We found the treatment ligands 23(S)-mCDCA GPBARA (3-(2-Chlorophenyl)-N-(4-chlorophenyl)-N,5-dimethylisoxazole-4-carboxamide) suppressed gene...

The gut microbiota has been strongly linked to gastrointestinal cancer, but the relationship between and esophageal cancer (EC) is still not fully understood. We conducted a 2-sample Mendelian randomization (MR) study unveil potential impact of intestinal microorganisms on EC in East Asian populations. In order delve deeper into causal EC, we MR analysis, utilizing 211 single nucleotide polymorphisms associated with microbiota, sourced from largest genome-wide association for our analysis....

Objective This study aims to assess the mesenchymal-epithelial transition factor’s (c-MET) prognostic value in oesophageal carcinoma (ESCA) through a meta-analysis and bioinformatics. Methods We analysed c-MET expression ESCA tissues using data from The Cancer Genome Atlas (TCGA) conducted evaluate its association with clinicopathological factors survival outcomes. included studies reporting hazard ratios (HRs) odds (ORs) for metastatic Results analysis revealed elevated ESCA, which was...

Primary gastric cancer (PGC) is the fourth most common malignant human and second leading cause of death worldwide.The majority subjects PGC diagnosed at a late stage, resulting in poor prognosis therapeutic outcome, largely attributable to dissemination tumor cells into circulation as circulating (CTCs) their formation distal tumor.Curcumin an active ingredient from rhizome plant Curcuma longa.Here, we assessed whether treatment with Curcumin may reduce incidence metastatic liver mice...

Abstract Background Protein inhibitor of activated STATs (PIAS) has pleiotropic biological effects, such as protein post-translational modification, transcriptional coregulation and gene editing. It is reported that PIAS family genes are also correlated with immune cells infiltration in cancers highlights their unnoticed role tumor progression. However, the relationship expression prognosis, cell infiltration, microenvironment, immunotherapy pan-cancer been rarely reported. Methods The...

Enriched environment (EE) can protect the brain against damages caused by an ischemic stroke; however, underlying mechanism remains elusive. Autophagy and mitochondria quality control are instrumental in pathogenesis of stroke. In this study, we investigated whether how autophagy contribute to protective effect EE acute phase cerebral ischemia-reperfusion injury.We exposed transient middle artery occlusion (tMCAO) mice or standard condition (SC) for 7 days then studied them neurological...

Stroke seriously affects human health. Many studies have shown that enriched environment (EE) can promote functional recovery after stroke, but the intrinsic mechanisms remain unclear. In order to study internal of EE involved in ischemic stroke and which mechanism plays a leading role limb function cerebral infarction, key proteins potentially neuronal protection synaptic remodeling penumbra been investigated. this study, adult C57BL/6 mice permanent middle artery occlusion (pMCAO) were...

Numerous studies have demonstrated that immune cell infiltration is a significant predictor in the prognosis of those with breast cancer. This study aimed to develop prognostic model for undifferentiated cancer using immune-related markers. Differentially expressed genes (DEGs) and factors were identified from The Cancer Genome Atlas (TCGA) database. immune-associated filtered GeneCards Least absolute shrinkage selection operator (LASSO) Cox proportional hazards regression employed select...

Although skeletal muscle is the main effector organ largely accounting for disability after stroke, considerably less attention paid to secondary abnormalities of stroke-related loss. It necessary explore mechanism atrophy stroke and further develop effective rehabilitation strategy. Here, we evaluated effects high-intensity interval (HIIT) versus moderate-intensity aerobic training (MOD) on physical function, mass, gene expression profile muscle. After model middle cerebral artery occlusion...

Human umbilical cord mesenchymal stem/stromal cells (hUMSCs) hold promise for treating acute liver failure (ALF). Here, we investigated the therapeutic effect of hUMSCs overexpressing hepatocyte nuclear factor 4α (HNF4α), a transcription important maintaining identity and hepatic functions, in ALF, compared with without overexpression HNF4α (CON‐hUMSCs). The were administered into mice via tail vein 24 h before exposure to lipopolysaccharide/ d ‐galactosamine (LPS/ ‐GalN) 6 by...

Genistein is a natural isoflavone with pharmacological or potentially anti-tumor properties. However, the resistance of cancer cells to genistein remains major obstacle. This study focused on mechanism implicated in pancreatic (PC) and action. First, key molecules signaling pathways related PC were explored using bioinformatics tools. DEP domain containing MTOR interacting protein (DEPTOR), typical inhibitor mammalian target rapamycin (mTOR) signaling, was predicted be poorly expressed...

Abstract Background Pancreatic adenocarcinoma (PAAD) is a nonimmunogenic tumor, and very little known about the relationship between host immune response patient survival. We aimed to develop an prognostic model (IPM) analyze its relevance tumor profiles of patients with PAAD. Methods investigated differentially expressed genes normal tissues in TCGA PAAD cohort. Immune-related were screened from highly variably weighted gene correlation network analysis (WGCNA) construct IPM. Then,...