A5030731365- Breast Cancer Treatment Studies
- Cancer-related Molecular Pathways
- Advanced Radiotherapy Techniques
- Breast Lesions and Carcinomas
- Glioma Diagnosis and Treatment
- Virus-based gene therapy research
- Breast Implant and Reconstruction
- Cancer Research and Treatments
- Management of metastatic bone disease
- Brain Metastases and Treatment
- Genetic Mapping and Diversity in Plants and Animals
- Nonmelanoma Skin Cancer Studies
- Developmental Biology and Gene Regulation
- Mitochondrial Function and Pathology
- Meningioma and schwannoma management
- Head and Neck Cancer Studies
- Immune Cell Function and Interaction
- Congenital heart defects research
- Adipose Tissue and Metabolism
- Histone Deacetylase Inhibitors Research
- interferon and immune responses
- Cancer and Skin Lesions
- Bone Tumor Diagnosis and Treatments
- Cardiomyopathy and Myosin Studies
- Effects of Radiation Exposure
Wake Forest University
2013-2024
Atrium Health Wake Forest Baptist
2012-2024
University of Colorado Denver
2018-2023
Arizona Oncology
2016-2019
Boston Children's Museum
2018
Boston Children's Hospital
2018
Johns Hopkins University
2018
University of Miami
2018
Sylvester Comprehensive Cancer Center
2018
Society of Surgical Oncology
2017
Studies in human systemic lupus erythematosus (SLE) suggest a possible role for histone deacetylases (HDACs) skewed gene expression and disease pathogenesis. We used the MRL-lpr/lpr murine model of to demonstrate that HDACs play key heightened levels both Th1 Th2 cytokine contribute disease. The availability specific HDAC inhibitors (HDIs) such as trichostatin A (TSA) suberonylanilide hydroxamic acid (SAHA) permits study regulation. Our results indicate HDIs downregulate IL-12, IFN-gamma,...
ABSTRACT Members of the basic helix-loop-helix (bHLH) family transcription factors have been shown to regulate growth and differentiation numerous cell types. Cell-typespecific bHLH proteins typically form heterodimers with ubiquitous proteins, such as E12, bind a DNA consensus sequence known an E-box. We used yeast two-hybrid system screen mouse embryo cDNA libraries for cDNAs encoding novel cell-type-specific that dimerize E12. One isolated encoded protein, called scleraxis. During...
Spine metastasis can be treated with high-dose radiation therapy advanced delivery technology for long-term tumor and pain control.
In the mouse embryo, splanchnic mesodermal cells of anterior heart field (AHF) migrate from pharynx to contribute early myocardium outflow tract (OT) and right ventricle (RV). Recent studies have attempted distinguish AHF other precardiac populations, determine genetic molecular mechanisms that regulate its development. Here, we used an Fgf8lacZ allele demonstrate Fgf8is expressed within developing AHF. addition, use both a hypomorphic Fgf8 (Fgf8neo) Cre-mediated gene ablation show is...
Cardiac and skeletal muscle critically depend on mitochondrial energy metabolism for their normal function. Recently, we showed that apoptosis-inducing factor (AIF), a protein implicated in programmed cell death, plays role respiration. However, the vivo consequences of AIF-regulated respiration resulting from loss-of-function mutation Aif are not known. Here, report tissue-specific deletion mouse. Mice which has been inactivated specifically cardiac exhibit impaired activity expression...
Abstract Inheritance of mitochondrial DNA (mtDNA) in Holstein cattle was characterized by pedigree analysis nucleotide sequence variation. mtDNA purified from leukocytes 174 individuals representing 35 independent maternal lineages, and analyzed for variation characterization restriction fragment length polymorphism direct determination. These data revealed 11 lineages which some seemingly were homoplasmic the reference at 364, whereas those other a variant this position. Both alternative...
To determine the maximum-tolerated dose (MTD) of radiation (RT) with concurrent temozolomide in patients newly diagnosed glioblastoma (GBM), to estimate their progression-free (PFS) and overall survival (OS), assess role (11)C methionine PET (MET-PET) imaging predicting recurrence.Intensity-modulated RT (IMRT) doses 66 81 Gy, assigned by time-to-event continual reassessment method, were delivered over 6 weeks daily (75 mg/m(2)) followed adjuvant cyclic (200 mg/m(2) d1-5 q28d ×6 cycles)....
Mastectomy is standard for recurrence of breast cancer after conservation therapy with whole irradiation. The emergence partial irradiation led to consideration its application reirradiation a second lumpectomy treatment in the ipsilateral breast.To assess effectiveness and adverse effects whether an acceptable alternative mastectomy.The NRG Oncology/Radiation Therapy Oncology Group 1014 trial phase 2, single-arm, prospective clinical 3-dimensional, conformal, external beam previous study...
In systemic lupus erythematosus (SLE), T helper cells exhibit increased and prolonged expression of cell-surface CD40 ligand (CD154), spontaneously overproduce interleukin-10 (IL-10), but underproduce interferon-gamma (IFN-γ). We tested the hypothesis that imbalance these gene products reflects skewed CD154 , IL-10 IFN- γ genes. Here, we demonstrate histone deacetylase inhibitor, trichostatin A, significantly down-regulated up-regulated in SLE cells. This reversal corrected aberrant...
The human epidermal growth factor receptor (EGFR) promoter is activated by both wild-type and tumorderived mutant p53.In this communication, we demonstrate that EGFR sequence requirements for transactivation p53 are different.Transient-expression assays with deletions identified a response element, 5-AGCTAGACGTCCGGGCAGCCCCCGGCG -3, from positions ؊265 to ؊239.Electrophoretic mobility shift analysis DNase I footprinting indicated binds specifically the element.Using circularly permuted DNA...
Radiotherapy (RT) is commonly used in the treatment of breast cancer and often, despite advances fractionated dosing schedules, produces undesirable skin toxicity. The purpose this study was to evaluate feasibility using a keratin-based topical cream, KeraStat® Cream (KC; KeraNetics, Inc., Winston Salem, NC, USA) manage symptoms radiation dermatitis (RD) patients undergoing RT.
AbstractThe wild-type p53 protein is a transcriptional activator implicated in the control of cellular growth-related gene expression. Here, using number different cell lines and transient-transfection–transcription assays, we demonstrate that at low levels, transactivates human proliferating nuclear antigen (PCNA) promoter. When expressed similar level, tumor-derived mutants did not transactivate PCNA We identified p53-binding site on promoter with which interacts sequence specifically....
ABSTRACT Scleraxis is a basic helix-loop-helix (bHLH) transcription factor shown previously to be expressed in developing chondrogenic cell lineages during embryogenesis. To investigate its function embryonic development, we produced scleraxis-null mice by gene targeting. Homozygous mutant embryos developed normally until the early egg cylinder stage (embryonic day 6.0), when they became growth-arrested and failed gastrulate. Consistent with this phenotype, scleraxis was found throughout...
Apoptosis-inducing factor (AIF) is an evolutionarily conserved, ubiquitously expressed flavoprotein with NADH oxidase activity that normally confined to mitochondria. In mammalian cells, AIF released from mitochondria in response apoptotic stimuli and translocates the nucleus where it thought bind DNA contribute chromatinolysis cell death a caspase-independent manner. Here we describe consequences of inactivating Aif early mouse embryo. Unexpectedly, found both apoptosis-dependent process...
Purpose This study examined an inflammatory biomarker, high-sensitivity C-reactive protein (hsCRP), in radiotherapy (RT)-induced early adverse skin reactions or toxicities breast cancer. Patients and Methods Between 2011 2013, 1,000 patients with cancer who underwent RT were evaluated prospectively for through the National Cancer Institute-funded Wake Forest University Community Clinical Oncology Program Research Base. Pre- post-RT plasma hsCRP levels Nursing Society toxicity criteria (0 to...