A5012501258- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Reproductive Physiology in Livestock
- Immune Response and Inflammation
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Reproductive tract infections research
- Hepatitis B Virus Studies
- Genetic and phenotypic traits in livestock
- Immune Cell Function and Interaction
- Syphilis Diagnosis and Treatment
- Effects of Environmental Stressors on Livestock
- Gut microbiota and health
- Respiratory viral infections research
- T-cell and B-cell Immunology
- Thyroid Disorders and Treatments
- Liver Disease Diagnosis and Treatment
- Viral Infections and Immunology Research
- Barrier Structure and Function Studies
- Reproductive Biology and Fertility
- Immunotherapy and Immune Responses
- Nutrition, Genetics, and Disease
- HIV-related health complications and treatments
- Animal Behavior and Welfare Studies
Purdue University West Lafayette
2020-2025
University of Saskatchewan
2014-2021
Purdue University Northwest
2021
Vancouver Infectious Diseases Centre
2016
University of Alberta
2008-2012
Hospital São Paulo
2007
Hospital Israelita Albert Einstein
2007
Tampere University
1998
Universidade de São Paulo
1993
Real Hospital Português
1991
The associations between six genetic polymorphisms in the hepatic lipase (HL) gene (LIPC) and variation postheparin HL activity fasting serum lipoproteins were evaluated 395 male Finnish coronary heart disease patients with HDL cholesterol concentrations </= 1.1 mmol/liter. LIPC promoter polymorphism at position -514 was highly significantly associated (P = 0.0000008), mean activities of 20.4, 17.5, 13.2 mumol free fatty acid/ml per hour subjects having C/C, C/T, T/T genotypes, respectively....
Reactive arthritis, an autoimmune disorder, occurs following gastrointestinal infection with invasive enteric pathogens, such as Salmonella enterica. Curli, extracellular, bacterial amyloid cross beta-sheet structure can trigger inflammatory responses by stimulating pattern recognition receptors. Here we show that S. Typhimurium produces curli amyloids in the cecum and colon of mice after natural oral infection, both acute chronic models. Production was associated increase anti-dsDNA...
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) can be transmitted across the maternal-fetal-interface from an infected gilt to her fetuses. Although fetal infection status disease outcomes vary, mechanisms are not completely understood. The objective was assess targeted placental structural transporter-related gene expression patterns. At day 85 of gestation pregnant pigs were challenged with PRRSV, at 12 days post maternal sows fetuses sacrificed, tissue...
Oral progestagen treatment extends the weaning-to-estrus interval (WEI) in weaned sows. Particularly lower parity sows, this allows recovery from lactational catabolism and improves sow productivity. However, optimal duration of contemporary dam-line sows is unclear. Therefore, (n = 749) over consecutive 3-wk periods June July classified as 2 3 (P2–3); 4, 5, 6 (P4–6); or 7 higher (P7+) were organized into breeding groups using 1 strategies: 1) oral for d before 12 after weaning (M14; n 249);...
To understand the fetal immune response to porcine reproductive and respiratory virus-2 (PRRSV) evaluate association with viability, pregnant gilts were challenged on gestation day 85 euthanized 21 days post infection. Based preservation status viral load in serum thymus, fetuses classified as either uninfected-viable (UNIF), high viable (HV-VIA), or meconium stained (HV-MEC) compared age matched control (CON) derived from mock infected gilts. Gene expression of IFNB, IFNG, CCL2, CCL5,...
Binding of ligand to B-cell membrane immunoglobulin (mIg) can lead activation a number distinct biologic responses, including altered expression genes encoding c-fos, c-myc, and Ia, as well proliferation immunologic tolerance. Tolerance could reflect functional uncoupling receptors from systems that generate intracellular second messengers (i.e., receptor desensitization). To better understand the molecular basis immune regulation, we examined ability mIg function signal transducer after...
We intended to assess how exposure of piglets deoxynivalenol (DON)-contaminated feed impacted their growth, immune response and gut development. Piglets were fed traditional Phase I, II III diets with the control group receiving 0.20–0.40 ppm DON (referred as Control group) treatment much higher level DON-contaminated wheat (3.30–3.80 ppm; referred group). Feeding a diet had no impact on average daily intake (ADFI) (p < 0.08) or gain (ADG) > 0.10) but it did significantly reduce body...
Newborn piglets are immunologically naïve and must receive passive immunity via colostrum within 24 hours to survive. Mechanisms by which the newborn piglet gut facilitates uptake of colostral cells, antibodies, proteins may include FcRn pIgR receptor‐mediated endocytosis paracellular transport between tight junctions (TJs). In present study, gene (FCGRT) was minimally expressed in 6‐week‐old jejunum but it at significantly higher levels ileum piglets. highly animals only neonatal gut....
Our goal was to develop a robust tagging method that can be used track bacterial strains in vivo To address this challenge, we adapted two existing systems: modular plasmid-based reporter system (pCS26) has been for high-throughput gene expression studies Salmonella and Escherichia coli Tn7 transposition. We generated kanamycin- chloramphenicol-resistant versions of pCS26 with luciferase, green fluorescent protein (GFP), mCherry reporters under the control σ(70)-dependent promoters provide...
The impact of late gestation PRRSV-2 infection is highly variable within a litter, with subset fetuses displaying varying degrees compromise following while others remain viable despite significant systemic viral load. To understand the underlying cause this variation, we examined susceptibility, distribution and non-lymphoid tissues. Samples brain, heart, kidney, liver, lung, skeletal muscle were obtained from pregnant gilts at day 86, presence CD163+ cells each tissue evaluated via...
Abstract Background Hypothyroidism is a common endocrine disruption observed in utero that adversely affects fetal growth and maturation leading to long-term impacts on health; however, the exact molecular mechanisms by which these deleterious effects occur are unknown. We hypothesize hypothyroidism during late gestation will disrupt cell cycle regulation tissue-specific manner. To evaluate this, eight pregnant gilts were dosed with either methimazole or an equivalent negative control days...
The biochemical basis of Ca2+ mobilization after anti-Ig binding to B cell Ag-R has been further characterized by flow cytometric analysis indo-1-loaded cells. ability distinguish intracellular release from extracellular influx using an calcium depletion-repletion approach allowed us study the relationship between these sources. Studies involving manipulation gradient across plasma membrane indicate that a significant portion response is preserved even when normal inwardly directed reversed....
The domestic pig is an important livestock species and there strong interest in the factors that affect development of viable embryos offspring this species. A limited understanding molecular mechanisms involved early embryonic has inhibited our ability to fully elucidate these factors. Next generation deep sequencing microarray technologies are powerful tools for delineation pathways developing embryo. Here we present a porcine-embryo-specific platform created from large expressed sequence...
Abstract The MAM domain‐containing 4 ( MAMDC 4) protein is associated with the unique endocytotic mechanism observed in intestine of mammals during immediate postnatal period. Transcriptional expression was substantially upregulated at birth both piglet jejunum and ileum its decreases after birth. found localized specifically to apical region luminal epithelium, however, lost day 10 15 ileum, respectively, not “fetal” enterocyte replacement. Although spatial variation subcellular...
Expression of panels candidate genes controlling myogenesis, angiogenesis and gender-specific imprinting development were analysed in embryonic, placental endometrial tissues recovered at Day 30 gestation from a subset primiparous sows that either feed restricted (Restrict; n = 17) or fed to appetite (Control; 15) during the last week previous lactation. Embryos also sex typed investigate gender bias response treatments. Average embryonic weight was lower Restrict compared with Control...
A pregnant gilt infected with porcine reproductive and respiratory syndrome virus (PRRSV) can transmit the to her fetuses across maternal-fetal-interface resulting in varying disease outcomes. However, mechanisms leading variation fetal outcome response PRRSV infection are not fully understood. Our objective was assess targeted immune-related gene expression patterns pathways placenta thymus elucidate molecular involved resistance/tolerance susceptibility of PRRSV2 infection. Fetuses were...
To better understand the host response to porcine reproductive and respiratory virus-2 (PRRSV2) we evaluated circulating thyroid hormone associated gene expression in a late gestation challenge model. Pregnant gilts were inoculated at day 85 fetal samples collected either 12 or 21 days post-infection (dpi). A subset of fetuses was selected for analysis based on viability viral load categorized as uninfected-viable (UNIF), high viable (HV-VIA) meconium stained (HV-MEC) compared with...
Porcine reproductive and respiratory syndrome virus (PRRSV) infection during late gestation negatively affects fetal development. The objective of this study was to identify the organs most severely impacted following infection, evaluate relationship between response phenotypes. RNA extracted from heart, liver, lung, thymus, kidney, spleen, loin muscle, collected viral challenge pregnant gilts. Initially, gene expression for three cell cycle promoters (CDK1, CDK2, CDK4) one inhibitor...
Porcine reproductive and respiratory syndrome virus (PRRSV) in late gestation causes a profound suppression of circulating maternal fetal thyroid hormone during critical window development. To understand this relationship, we evaluated metabolism at the maternal-fetal interface within tissues, along with metabolite levels serum. Fetuses were classified using an established model based on viral load serum thymus, preservation status, including uninfected (UNIF), high-viral viable (HV-VIA),...