A5101606355- Sugarcane Cultivation and Processing
- Biofuel production and bioconversion
- Natural Products and Biological Research
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Rice Cultivation and Yield Improvement
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Bioenergy crop production and management
- Spectroscopy and Chemometric Analyses
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Plant and animal studies
- Genetics and Plant Breeding
- Power Systems and Renewable Energy
- Cuban History and Society
- Banana Cultivation and Research
- Studies on Chitinases and Chitosanases
- Plant pathogens and resistance mechanisms
Fujian Agriculture and Forestry University
2016-2025
Guangxi University
2014-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Guangdong Provincial Center for Disease Control and Prevention
2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2014-2024
Fanjingshan National Nature Reserve
2009
Abstract Sugarcane is a vital crop with significant economic and industrial value. However, the cultivated sugarcane’s ultra-complex genome still needs to be resolved due its high ploidy extensive recombination between two subgenomes. Here, we generate chromosomal-scale, haplotype-resolved assembly for hybrid sugarcane cultivar ZZ1. This contains 10.4 Gb genomic sequences 68,509 annotated genes defined alleles in sub-genomes distributed 99 original 15 recombined chromosomes. RNA-seq data...
Abstract Background APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play essential roles in plant growth, development, metabolism, and responses to biotic abiotic stresses. However, few studies concerning AP2/ERF genes sugarcane which are the most critical sugar energy crops worldwide. Results A total of 218 were identified Saccharum spontaneum genome. Phylogenetic analysis showed that these could be divided into four groups, including 43 AP2s, 160 ERFs...
Drought is the main abiotic stress that constrains sugarcane growth and production. To understand molecular mechanisms govern drought stress, we performed a comprehensive comparative analysis of physiological changes transcriptome dynamics related to highly drought-resistant (ROC22, cultivated genotype) weakly (Badila, wild sugarcane, in time-course experiment (0 h, 4 8 16 h 32 h). Physiological examination reviewed ROC22, which shows superior tolerance relative Badila, has high performance...
Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop haplotype-specific fluorescence situ hybridization (FISH) technique that allows visualization COs directly on metaphase chromosomes. Oligonucleotides (oligos) specific to chromosome 10 maize inbreds B73 Mo17, respectively, are synthesized labeled as FISH probes. The parental recombinant x Mo17 F1 hybrids F2 progenies can be...
Karyotypes provide key cytogenetic information on the phylogenetic relationships and evolutionary origins in related eukaryotic species. Despite our knowledge of chromosome numbers sugarcane its wild relatives, composition evolution among species Saccharum complex have been elusive owing to polyploidy large chromosomes these Oligonucleotide-based painting has become a powerful tool studies especially for plant with chromosomes. We developed oligo-based probes all 10 officinarum (2n = 8x 80)....
Sugarcane (Saccharum spp. hybrid) is frequently affected by seasonal drought, which causes substantial declines in quality and yield. To understand the drought resistance mechanisms of S. officinarum, main species modern sugarcane, at a molecular level, we carried out comparative analysis transcriptome metabolome profiling sugarcane variety Badila under stress (DS). Compared with control group (CG) plants, plants exposed to DS had 13,744 (6663 up-regulated 7081 down-regulated) differentially...
Erianthus arundinaceus is a valuable source of agronomic traits for sugarcane improvement such as ratoonability, biomass, vigor, tolerance to drought and water logging, well resistance pests disease. To investigate the introgression E. genome into sugarcane, five intergeneric F1 hybrids between S. officinarum 13 their BC1 progeny were studied using genomic in situ hybridization (GISH) technique. In doing so, we assessed chromosome composition transmission these plants. All aneuploidy,...
The study of specialized interactions between species is crucial to our understanding processes in evolutionary ecology due their profound effect on life cycles and diversification. Obligate pollination by a single wasp rare Orchidaceae except with sexually deceptive flowers that are pollinated exclusively male insects. object this was document the food-deceptive Coelogyne fimbriata, female wasps. Field observations experiments were conducted two populations C. fimbriata. Floral phenology...
Erianthus arundinaceus (E. arundinaceus) has many desirable agronomic traits for sugarcane improvement, such as high biomass, vigor, rationing ability, tolerance to drought, and water logging, well resistance pests disease. To investigate the introgression of E. genome into in higher generations, intergeneric BC2 BC3 progeny generated between Saccharum spp. were studied using genomic situ hybridization (GISH) technique. The results showed that generations resulted from n + chromosome...
Summary Meiotic chromosome pairing between homoeologous chromosomes was reported in many nascent allopolyploids. Homoeologous is gradually eliminated and replaced by exclusive homologous well‐established allopolyploids, an evolutionary process referred to as the diploidization of A fundamental question allopolyploids whether what extent DNA sequence variation among contribute establishment pairing. We developed aneuploid tetraploid maize lines that contain three copies 10 derived from inbred...
A genetic diversity analysis and identification of plant germplasms varieties are important necessary for breeding. Deoxyribonucleotide (DNA) fingerprints based on genomic molecular markers play an role in accurate germplasm identification. In this study, Specific-Locus Amplified Fragment Sequencing (SLAF-seq) was conducted a sugarcane population with 103 cultivated wild accessions. total, 105,325 single nucleotide polymorphisms (SNPs) were called successfully to analyze components...
Sugarcane (Saccharum spp. hybrid) is an important crop for sugar and biofuels, often suffers from water shortages during growth. Currently, there limited knowledge concerning the molecular mechanism involved in sugarcane response to drought stress (DS) whether chitooligosaccharide could alleviate DS. Here, we carried out a combined transcriptome metabolome of three different treatment groups: control group (CG), DS group, + (COS). A total 12,275 (6404 up-regulated 5871 down-regulated)...
Interspecific hybridization is an effective strategy for germplasm innovation in sugarcane. Nobilization refers to the breeding theory of development and utilization wild germplasm. Saccharum spontaneum main donor resistance adaptive genes nobilization process. Chromosome transfer sugarcane complicated; thus, research different inheritance patterns can provide guidance optimal breeding. Through chromosome counting genomic situ hybridization, we found that six clones with 80 chromosomes were...
Abstract Sugarcane pokkah boeng disease (PBD), caused by a Fusarium species complex, is one of the most critical diseases causing significant economic loss sugarcane production in China. It important to standardize evaluation method for screening cultivars resistance PBD. Field trials were conducted investigate occurrence PBD different months and growth stages from 2018 2019 evaluate 169 genotypes 2022. mainly occurred early middle elongation at plant height approximately 1–2 m. The severe...
Sugarcane twisted leaf disease, caused by Phoma sp., was first reported in Guangxi, China, 2012, when more than 5% of sugarcane infected the field. Three single-spore isolates were recovered from symptomatic leaves. Sequences five fungal loci, 28S nrDNA (LSU), 18S (SSU), internal transcribed spacer regions 1 and 2 5.8S (ITS), β-tubulin (TUB), translation elongation factor alpha (TEF-α) amplified disease-associated isolates. The disease pathogen identified formally described as sorghina var....
The 45S ribosomal DNA (rDNA) units are separated by an intergenic spacer (IGS) containing the signals for transcription and processing of rRNAs. For first time, we sequenced analyzed entire IGS region from three original species within genus Saccharum, including S. spontaneum, robustum, officinarum in this study. We have compared organization Saccharum. these showed similar overall organizations comprised putative functional elements needed rRNA gene activity as well a non-transcribed (NTS),...
Abstract Background Sugarcane ( Saccharum officinarum L.) is an economically important crop with stalks as the harvest organs. Improvement in stalk quality deemed a promising strategy for enhancing sugarcane production. However, lack of efficient approaches systematic evaluation germplasm largely limits improvements quality. This study designed to develop near-infrared spectroscopy (NIRS) assay high-throughput phenotyping quality, thereby providing feasible solution precise germplasm....
The near-infrared spectroscopy (NIRS) has been used for efficient characterization and rapid assay of biomass saccharification in other energy plants, but its application sugarcane is not reported yet. current study collected a total 541 accessions to take an online NIRS assay. Among these collections, we observed large variations digestibility, particularly fermentable hexose sugar yield from fresh stalks, which were detected ranging 69.88 239.86 kg t-1 66.56–228.55 respectively. Using the...
The identification of germplasm resources is an important aspect sugarcane breeding. aim this study was to introduce a new method for identifying Saccharum spontaneum and its progeny. First, we cloned sequenced nuclear ribosomal DNA internal transcribed spacer (nrDNA-ITS) sequences from 20 germplasms. Analysis these nrDNA-ITS showed stable mutation at base 89. Primers (FO13, RO13, FI16, RI16) were then designed tetra-primer amplification refractory system (ARMS) PCR based on mutations 89 the...
Abstract Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus clones derived from these backcrosses to were used study the transmission of E . chromosomes by genomic in situ hybridization (GISH). True hybrid progenies precisely identified using PCR with a primer pair, AGRP52/53. The results showed that AGRP52/53 was an -specific pair could be as molecular marker assist breeding. EaHN92, 364 bp tandem repeat satellite DNA sequence, cloned clone HN92–105 AGRP52/53, localized...