A5011473898- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- RNA modifications and cancer
- Biochemical and Molecular Research
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- Immune cells in cancer
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Cancer, Hypoxia, and Metabolism
- Regulation of Appetite and Obesity
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Immune Cell Function and Interaction
Yale University
2014-2020
Shanghai Jiao Tong University
2011-2016
University of Bonn
2011
Brookhaven National Laboratory
2011
Abstract Male Sterile2 (MS2) is predicted to encode a fatty acid reductase required for pollen wall development in Arabidopsis (Arabidopsis thaliana). Transient expression of MS2 tobacco (Nicotiana benthamiana) leaves resulted the accumulation significant levels C16 and C18 alcohols. Expression fused with green fluorescent protein revealed that an amino-terminal transit peptide targets plastids. The plastidial localization biologically important because genetic complementation ms2 homozygous...
Many intracellular proteins are reversibly modified by O-linked GlcNAc (O-GlcNAc), a post-translational modification that dynamically regulates fundamental cellular processes in response to diverse environmental cues. Accumulating evidence indicates both excess and deficiency of protein O-GlcNAcylation can have deleterious effects on the cell, suggesting maintenance O-GlcNAc homeostasis is essential for proper function. However, mechanisms through which maintained physiologic state altered...
Abstract Excessive visceral fat accumulation is a primary risk factor for metabolically unhealthy obesity and related diseases. The highly susceptible to the availability of external nutrients. Nutrient flux into hexosamine biosynthetic pathway leads protein posttranslational modification by O -linked β-N-acetylglucosamine ( -GlcNAc) moieties. -GlcNAc transferase (OGT) responsible addition GlcNAc moieties target proteins. Here, we report that inducible deletion adipose OGT causes rapid loss...
Significance Overnutrition leads to metabolic disorders including obesity and diabetes. Studies have shown that enhanced inflammation is an essential player in the progression of diseases. However, how immune cells sense nutritional status contribute whole-body metabolism are largely unknown. Protein O -linked β- N -acetylglucosamine ( -GlcNAc) modification thought be a sensor modulates cell signaling. Here, we show overnutrition stimulates -GlcNAc signaling macrophages. suppresses...
Palatable foods (fat and sweet) induce hyperphagia, facilitate the development of obesity. Whether how overnutrition increases appetite through adipose-to-brain axis is unclear. O-linked beta-D-N-acetylglucosamine (O-GlcNAc) transferase (OGT) couples nutrient cues to O-GlcNAcylation intracellular proteins at serine/threonine residues. Chronic dysregulation O-GlcNAc signaling contributes metabolic diseases. Here we show that adipocyte OGT essential for high fat diet-induced but dispensable...
Abstract Rare codons generally arrest translation due to rarity of their cognate tRNAs. This property rare can be utilized regulate protein expression. In this study, a linear relationship was found between expression levels genes and copy numbers inserted within them. Based on discovery, we constructed molecular device in Escherichia coli using the codon AGG, its tRNA (tRNA Arg (CCU)), modified Asp (GUC → CCU), truncated aspartyl-tRNA synthetase (TDRS) switch reporter or off as well...