A5049287519- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Radiomics and Machine Learning in Medical Imaging
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Pancreatic function and diabetes
- Kruppel-like factors research
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- Zebrafish Biomedical Research Applications
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Nutritional Studies and Diet
- Cancer, Hypoxia, and Metabolism
- Selenium in Biological Systems
- Chemokine receptors and signaling
- Planarian Biology and Electrostimulation
- Plant and Biological Electrophysiology Studies
- Protein Degradation and Inhibitors
- Marine Invertebrate Physiology and Ecology
- Folate and B Vitamins Research
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
Michigan State University
2025
Wayne State University
2025
Henry Ford Hospital
2023-2025
Jinggangshan University
2020-2024
Henry Ford Health System
2024
Beth Israel Deaconess Medical Center
2016-2024
Michigan United
2024
Cancer Research Institute
2017-2024
Nanjing Normal University
2023
Tianjin Medical University
2020-2023
We used gene targeting techniques to produce mice lacking the invariant chain associated with major histocompatibility complex (MHC) class II molecules. Cells from these show a dramatic reduction in surface II, resulting both defective association of alpha and beta chains markedly decreased post-Golgi transport. The few alpha/beta heterodimers reaching cell behave as if empty or occupied by an easily displaced peptide, display distinct structure. Mutant spleen cells are their ability present...
Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms three-dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form multicellular spheres with a hollow central lumen. However, mechanophysical properties associated are poorly understood. We performed multidimensional live-cell imaging analysis track morphogenetic process starting single development multicellular, spherical structure composed...
Patient-derived organoid models are proving to be a powerful platform for both basic and translational studies. Here we conduct methodical analysis of pancreatic ductal adenocarcinoma (PDAC) tumor drug response in paired patient-derived xenograft (PDX) PDX-derived (PXO) grown under WNT-free culture conditions. We report specific relationship between area the curve value dose vivo growth, irrespective treatment. In addition, analyzed glycome PDX PXO demonstrate that PXOs recapitulate glycan...
Targeting carbonic anhydrase IX acidifies intracellular pH, disrupts redox homeostasis, and creates vulnerability to ferroptosis.
The exocrine pancreas, consisting of ducts and acini, is the site origin pancreatitis pancreatic ductal adenocarcinoma (PDAC). Our understanding genesis progression human diseases, including PDAC, limited because challenges in maintaining acinar cells culture. Here we report induction pluripotent stem toward organoids that recapitulate properties neonatal pancreas. Expression PDAC-associated oncogene GNASR201C induces cystic growth more effectively than organoids, whereas KRASG12D effective...
Abstract Purpose: Pancreatic ductal adenocarcinoma (PDAC) remains a significant health issue. For most patients, there are no options for targeted therapy, and existing treatments limited by toxicity. The HOPE trial (Harnessing Organoids PErsonalized Therapy) was pilot feasibility aiming to prospectively generate patient-derived organoids (PDO) from patients with PDAC test their drug sensitivity correlation clinical outcomes. Experimental Design: PDOs were established heterogeneous...
Microbial sulfur metabolism plays crucial roles in various food and alcoholic beverage fermentations. 3-(Methylthio)-1-propanol dimethyl disulfide are important compounds fermented foods beverages. Here, we studied the dynamics of these two during spontaneous Chinese liquor fermentation. The reached maximum concentration at day 10 production rate 3. Metatranscriptomic analysis days 3 revealed a total 354 metabolically active microorganisms. Saccharomyces Lactobacillus were identified as core...
Purpose: Since drug responses vary between patients, it is crucial to develop pre-clinical or co-clinical strategies that forecast patient response. In this study, we tested whether RNA-based therapeutics were suitable for personalized medicine by using patient-derived-organoid (PDO) and patient-derived-xenograft (PDX) models.Experimental Design: We performed microRNA (miRNA) profiling of PDX samples determine the status miRNA deregulation in individual pancreatic ductal adenocarcinoma...
Long non-coding RNA (lncRNA) and exosomes are involved in the pathological process of Alzheimer's disease (AD), changes which usually first observed entorhinal cortex hippocampus. The aim present study was to determine whether measurement plasma exosomal lncRNA combined with image data hippocampus could be used as a biomarker AD. A total 72 patients AD 62 controls were recruited, expression levels several lncRNAs assessed. Of recruited participants, 22 26 received brain 3D‑BRAVO sequence...
Background Tumor-specific cytotoxic T cells and cell receptors are effective tools for cancer immunotherapy. Most efforts to identify them rely on known antigens or lymphocytes that have infiltrated into the tumor bed. Approaches empirically tumor-targeting by exploiting all expressed surfaces not well developed most carcinomas, including pancreatic cancer. Methods Autologous organoids were stimulated with from patients’ peripheral blood 2 weeks generate organoid-primed (opT) cells. opT...
Cytokinin oxidase/dehydrogenases (CKXs) play a critical role in the irreversible degradation of cytokinins, thereby regulating plant growth and development. Brassica napus is one most widely cultivated oilseed crops worldwide. With completion whole-genome sequencing B. napus, genome-wide identification expression analysis BnCKX gene family has become technically feasible. In this study, we identified 23 genes analyzed their phylogenetic relationships, structures, conserved motifs, protein...
The study identifies an opportunity to discover a PKA-independent pathway downstream of oncogene GNAS for managing IPMN lesions and their progression PDAC.
Metastasis development is the leading cause of cancer-related mortality in pancreatic ductal adenocarcinoma (PDAC) and yet, few preclinical systems to recapitulate its full spreading process are available. Thus, modeling tumor progression metastasis urgently needed. In this work, we describe generation highly metastatic PDAC patient-derived xenograft (PDX) mouse models subsequent single-cell RNA-sequencing (RNA-seq) circulating cells (CTC), isolated by human HLA sorting, identify altered...
Diet and chronic inflammation might play a major role in the pathogenesis of mild cognitive impairment (MCI). In addition, peripheral blood leukocyte telomere length (LTL) mitochondrial DNA copy number (mtDNAcn) mediate relationship between MCI risk. The purpose present study is to evaluate whether inflammatory potential diet assessed by dietary index (DII), inflammation, LTL, mtDNAcn were associated with risk MCI.A population-based cohort was conducted total 2944 participants. During median...
Abstract Purpose: Pancreatic ductal adenocarcinoma (PDAC) patients with tumors enriched for the basal-like molecular subtype exhibit enhanced resistance to standard-of-care treatments and have significantly worse overall survival compared classic subtype–enriched tumors. It is important develop genomic resources, enabling identification of novel putative targets in a statistically rigorous manner. Experimental Design: We compiled single-cell RNA sequencing (scRNA-seq) atlas human pancreas...
<div>Abstract<p>Pancreatic cancer is the third leading cause of cancer-related death in United States. Black or African American patients have a higher incidence pancreatic compared with other racial groups. It unclear whether distinct molecular mechanisms are involved development different To identify tumor features that distinctly associated race and White ductal adenocarcinoma (the main subtype cancer), we analyzed deidentified patient records, including sequencing data...
<p>Characteristics of patients included in this study. Numbers the brackets represent percentages designated groups</p>