A5090332076- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Asthma and respiratory diseases
- RNA modifications and cancer
- Food Allergy and Anaphylaxis Research
- Reproductive System and Pregnancy
- Tumors and Oncological Cases
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Allergic Rhinitis and Sensitization
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
Jackson Laboratory
2019-2024
Cross-linking of high-affinity immunoglobulin E (IgE) results in the life-threatening allergic reaction anaphylaxis. Yet cellular mechanisms that induce B cells to produce IgE response allergens remain poorly understood. T follicular helper (TFH) direct affinity and isotype antibodies produced by cells. Although TFH cell-derived interleukin-4 (IL-4) is necessary for production, it not sufficient. We report a rare population IL-13-producing present mice humans with allergens, but when...
Immune cell activation assays have been widely used for immune monitoring and understanding disease mechanisms. However, these are typically limited in scope. A holistic study of circulating responses to different activators is lacking. Here we developed a cost-effective high-throughput multiplexed single-cell RNA-seq combined with epitope tagging (CITE-seq) determine how classic T cells (anti-CD3 coupled anti-CD28) or monocytes (LPS) alter the composition transcriptional profiles peripheral...
Although epithelial-mesenchymal transition (EMT) is a common feature of fibrotic lung disease, its role in fibrogenesis controversial. Recently, aberrant basaloid cells were identified tissue as novel epithelial cell type displaying partial EMT phenotype. The developmental origin these remains unknown. To elucidate the development from bronchial epithelium, we mapped EMT-induced transcriptional changes at population and single-cell levels. Human grown submerged or air-liquid interface (ALI)...
The genome is pervasively transcribed to produce a vast array of non-coding RNAs (ncRNAs). Long (lncRNAs) are transcripts >200 nucleotides and best known for their ability regulate gene expression. Enhancer (eRNAs) subclass lncRNAs that synthesized from enhancer regions have also been shown coordinate biological function significance most eRNAs remain be determined. Epithelial mesenchymal transition (EMT) ubiquitous cellular process occurs during migration, homeostasis, fibrosis,...
Abstract Osteosarcoma is the most common malignant bone tumor in children, characterized by a high degree of genomic instability, resulting copy-number alterations and rearrangements without disease-defining recurrent mutations. Clinical trials based on molecular characterization have failed to find new effective therapies or improve outcomes over last 40 years. To better understand immune microenvironment osteosarcoma, we performed single-cell RNA sequencing six biopsy samples, combined...
Identifying host genetic factors modulating immune checkpoint inhibitor (ICI) efficacy has been experimentally challenging because of variations in both and tumor genomes, differences the microbiome, patient life exposures. Utilizing Collaborative Cross (CC) multi-parent mouse resource population, we developed an approach that fixes genomic configuration while varying genetics. With this approach, discovered response to anti-PD-1 (aPD1) immunotherapy was significantly heritable four distinct...
Abstract DOCK8 deficient patients have elevated food specific IgE and severe allergy. In Dock8-/- mice, we showed that T cell intrinsic restrains the development of IL-13 expressing follicular helper (Tfh13) cells drive anaphylactic production. Oral exposure to results in tolerance, yet loss (T-Dock8-/-) is sufficient Tfh13 differentiation peanut IgE. How prevents unknown. human cells, promotes STAT3 phosphorylation, an inhibitor GATA3. We show mouse activity STAT3-/- differentiate into...
Abstract Immune cell activation assays have been widely used for immune monitoring and understanding disease mechanisms. However, these are typically limited in scope. A holistic study of circulating responses to different activators is lacking. Here we developed a cost-effective high-throughput multiplexed single-cell RNA-seq combined with epitope tagging (CITE-seq) determine how classic T cells (anti-CD3 coupled anti-CD28) or monocytes (LPS) alter the composition transcriptional profiles...
The purpose of this protocol is to produce single nuclei from frozen human tissues for downstream assaying with the 10x Genomics Multiome assay. This has been demonstrated using Human placenta tissue as well Glioblastoma. modified Sigma Aldrich Nuclei Isolation Kit: EZ Prep and Chromium Demonstrated Single Cell ATAC + Gene Expression Sequencing (CG000365).
The purpose of this protocol is to produce single nuclei from frozen human tissues for downstream assaying with the 10x Genomics Multiome assay. This has been demonstrated using Human placenta tissue as well Glioblastoma. modified Sigma Aldrich Nuclei Isolation Kit: EZ Prep and Chromium Demonstrated Single Cell ATAC + Gene Expression Sequencing (CG000365).
The purpose of this protocol is to produce single nuclei from frozen human tissues for downstream assaying with the 10x Genomics Multiome assay. This has been demonstrated using Human placenta tissue as well Glioblastoma. modified Sigma Aldrich Nuclei Isolation Kit: EZ Prep and Chromium Demonstrated Single Cell ATAC + Gene Expression Sequencing (CG000365).
The purpose of this protocol is to produce single nuclei from frozen human tissues for downstream assaying with the 10x Genomics Multiome assay. This has been demonstrated using Human placenta tissue as well Glioblastoma. modified Sigma Aldrich Nuclei Isolation Kit: EZ Prep and Chromium Demonstrated Single Cell ATAC + Gene Expression Sequencing (CG000365).
Described here is the workflow used by JAX Single Cell Biology lab to generate single cell transcriptomic libraries from human placenta samples collected at UCSD HuBMAP Female Reproductive Tissue Mapping Center.
The purpose of this protocol is to produce single nuclei from frozen human tissues for downstream assaying with the 10x Genomics Multiome assay. This has been demonstrated using Human placenta tissue as well Glioblastoma. modified Sigma Aldrich Nuclei Isolation Kit: EZ Prep and Chromium Demonstrated Single Cell ATAC + Gene Expression Sequencing (CG000365).
The purpose of this protocol is to produce single nuclei from frozen human tissues for downstream assaying with the 10x Genomics Multiome assay. This has been demonstrated using Human placenta tissue as well Glioblastoma. modified Sigma Aldrich Nuclei Isolation Kit: EZ Prep and Chromium Demonstrated Single Cell ATAC + Gene Expression Sequencing (CG000365).