A5082988605- Endoplasmic Reticulum Stress and Disease
- Cytokine Signaling Pathways and Interactions
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Antioxidant Activity and Oxidative Stress
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Toxic Organic Pollutants Impact
- RNA Interference and Gene Delivery
- Pomegranate: compositions and health benefits
- Cancer Mechanisms and Therapy
- Phytochemicals and Antioxidant Activities
- RNA regulation and disease
- Nuclear Structure and Function
- Vitamin C and Antioxidants Research
- Natural Antidiabetic Agents Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genomics, phytochemicals, and oxidative stress
- RNA Research and Splicing
- Galectins and Cancer Biology
- Ubiquitin and proteasome pathways
Sapienza University of Rome
2016-2025
Istituto Pasteur
2004-2019
Biochemical Society
2009
Institute of Molecular Biology and Pathology
1986-2007
To study the role of oligosaccharides on properties glycoproteins, five glycoproteins (yeast external invertase, bovine serum fetuin, glucoamylase from Aspergillus niger, and chicken egg white ovotransferrin avidin) previously established glycan patterns were purified to homogeneity deglycosylated with endo- exo-glycosidases in native conditions. Thermal stability conformational changes measured by high-resolution differential scanning microcalorimetry circular dicroism spectroscopy before...
Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which are antioxidant and antidiabetic. In the present study, an ethyl acetate extract, obtained by Soxhlet from Dente di Cavallo DC2 pomegranate (PGE) characterized contain 4% w/w ellagic acid, has been evaluated for its hypoglycemic, antiglycation, antioxidative cytoprotective in order provide possible evidence future nutraceutical applications. The α-amylase α-glucosidase enzyme inhibition,...
Prostate cancer (PCa) is a multifactorial disease characterized by the aberrant activity of different regulatory pathways. STAT3 protein mediates some these pathways and its activation implicated in modulation several metabolic enzymes. A bioinformatic analysis indicated binding site upstream region SHMT2 gene. We demonstrated that LNCaP, PCa cells’ expression upregulated JAK2/STAT3 canonical pathway upon IL-6 stimulation. Activation SHTM2 leads to decrease serine levels, pushing PKM2...
Abstract Protein disulfide isomerase ERp57 is localized predominantly in the endoplasmic reticulum, but also present cytosol and, according to preliminary evidence, nucleus of avian cells. Conclusive evidence its nuclear localization and interaction with DNA vivo mammalian cells provided here on basis DNA–protein cross‐linking experiments performed two different agents viable HeLa 3T3 Nuclear could be detected by immunofluorescence cells, where it showed an intracellular distribution clearly...
Abstract The protein ERp57/GRP58 is a member of the disulfide isomerase family and also glucose‐regulated protein, which, together with other GRPs, induced by variety cellular stress conditions. mainly located in endoplasmic reticulum (ER), but has been found cytoplasm nucleus, where it can bind DNA. In order to identify possible correlation between stress‐response nuclear location ERp57/GRP58, its binding sites on DNA HeLa cells have searched chromatin immunoprecipitation cloning...
Abstract In patients with rheumatoid arthritis (RA) a decrease in the terminal galactose content of N‐linked glycans Fc region agalactosyl immunoglobulin G (IgG) (G0) occurs. The aim this study was to evaluate, for first time, effect infliximab, new monoclonal antibody treatment RA, on phenomenon. A total 19 active RA were treated intravenous infliximab (3 mg/kg) combination methotrexate (MTX) (10–20 mg). IgG purified from their serum by caprylic acid. Analysis glycosylation performed lectin...
STAT3 is an oncoprotein overexpressed in different types of tumors, including prostate cancer (PCa), and its activity modulated by a variety post-translational modifications (PTMs). Prostate represents the most common diagnosed men, each phase tumor progression displays specific cellular conditions: inflammation predominant tumor's early stage, whereas oxidative stress typical clinically advanced PCa. The aim this research to assess correspondence between stimulus-specificity PTMs definite...
Cholangiocarcinoma (CCA) is an aggressive group of biliary tract cancers, characterized by late diagnosis, low effective chemotherapies, multidrug resistance, and poor outcomes. In the attempt to identify new therapeutic strategies for CCA, we studied antiproliferative activity a combination between doxorubicin natural sesquiterpene β-caryophyllene in cholangiocarcinoma Mz-ChA-1 cells nonmalignant H69 cholangiocytes, under both long-term metronomic schedules. The modulation STAT3 signaling,...
Organochlorine pesticides (OCPs) belong to a heterogeneous class of organic compounds blacklisted by the Stockholm Convention in 2009 due their harmful impact on human health. Among OCPs, β-hexachlorocyclohexane (β-HCH) is one most widespread and, at same time, poorly studied environmental contaminant. Due its physicochemical properties, β-HCH hazardous all HCH isomers; therefore, clarifying mechanisms underlying molecular action could provide further elements draw biochemical profile this...
A combination of anticancer drugs and chemosensitizing agents has been approached as a promising strategy to potentiate chemotherapy reduce toxicity in aggressive chemoresistant cancers, like hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), pancreatic ductal adenocarcinoma (PDAC). In the present study, ability caryophyllane sesquiterpenes sorafenib efficacy was studied HCC, CCA, PDAC cell models, focusing on modulation STAT3 signaling ABC transporters; tolerability studies normal...
Abstract In the melanoma M14 cell line, we found that antimetastatic protein NM23/nucleoside diphosphate kinase binds to promoters of oncogene cMYC and P53, a gene often mutated in human cancer (Cervoni et al. [2006] J. Cell. Biochem. 98:421–428). further study, find now IFI16, transcriptional repressor, both G‐rich fragment also NM23/NDPK. These fragments possess non‐B DNA structures. Moreover, by sequential chromatin immunoprecipitation (re‐ChIP) show two proteins (IFI16 NM23/NDPK) are...