A5010417891- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Pharmaceutical and Antibiotic Environmental Impacts
- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Diet, Metabolism, and Disease
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Genomics, phytochemicals, and oxidative stress
- Liver Disease Diagnosis and Treatment
- Genetic Mapping and Diversity in Plants and Animals
- Plant responses to water stress
- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Nitrogen and Sulfur Effects on Brassica
- Advanced Technologies in Various Fields
- Water Quality Monitoring and Analysis
- Bioactive Compounds and Antitumor Agents
- Bioinformatics and Genomic Networks
- Plant biochemistry and biosynthesis
- Circular RNAs in diseases
- Virus-based gene therapy research
- Biological and pharmacological studies of plants
Huazhong Agricultural University
2017-2025
Kunming University of Science and Technology
2022-2023
Nanjing Agricultural University
2021-2022
Northeast Agricultural University
2022
Air Force Medical University
2020
UCLA Medical Center
2019
Harbor–UCLA Medical Center
2019
Princeton University
2012-2013
Howard Hughes Medical Institute
2010-2012
Abstract Different horticultural types of lettuce exhibit tremendous morphological variation. However, the molecular basis for domestication and divergence among different remains unknown. Here, we report RNA sequencing 240 accessions sampled from major wild relatives, generating 1.1 million single-nucleotide polymorphisms (SNPs). Demographic modeling indicates that there was a single event lettuce. We identify list regions as putative selective sweeps occurred during divergence,...
Many questions about the genetic basis of complex traits remain unanswered. This is in part due to low statistical power traditional mapping studies. We used a statistically powerful approach, extreme QTL (X-QTL), identify resistance 13 chemicals all 6 pairwise crosses four ecologically and genetically diverse yeast strains, we detected total more than 800 loci. found that number loci each experiment was primarily function trait (explaining 46% variance) rather cross (11%), suggesting level...
Significance Heading, which facilitates harvest and contributes to taste shelf life, is an important trait in some vegetable crops. However, litter known about its genetic molecular mechanism. Here, we report the cloning of a key regulator ( LsKN1 ) heading lettuce, homologous knotted 1 KN1 gene maize. The upregulated by insertion CACTA-like transposon. Its enhanced expression suppressed LsAS1 consequently altered leaf polarity led phenotype.
Lettuce is a globally important leafy vegetable that exhibits diverse horticultural types and strong population structure, which complicates genetic analyses. To address this challenge, we develop the first multi-parent, advanced generation inter-cross (MAGIC) for lettuce using 16 founder lines. Whole-genome sequencing of lines 381 inbred progeny reveal minimal enabling informative genome-wide association studies (GWAS). GWAS MAGIC identifies numerous loci associated with key agricultural...
Lettuce (Lactuca sativa) is one of the most important vegetable crops in world. As a leafy vegetable, polymorphism lettuce leaves from dark to pale green an trait. However, genetic and molecular mechanisms underlying such variations remain poorly understood. In this study, major locus controlling dark- pale-green was identified using genome-wide association studies (GWAS). This then fine mapped interval 5375 bp on chromosome 4 segregating population containing 2480 progeny. Only gene,...
Abstract Whole-genome sequencing, particularly in fungi, has progressed at a tremendous rate. More difficult, however, is experimental testing of the inferences about gene function that can be drawn from comparative sequence analysis alone. We present genome-wide functional characterization sequenced but experimentally understudied budding yeast, Saccharomyces bayanus var. uvarum (henceforth referred to as S. bayanus), allowing us map changes over 20 million years separate this organism...
Abstract The mechanisms underlying leafy heads in vegetables are poorly understood. Here, we cloned a quantitative trait locus (QTL) controlling lettuce (Lactuca sativa). QTL encodes transcription factor, SAWTOOTH 1 (LsSAW1), which has BEL1-like homeodomain and is homolog of Arabidopsis thaliana. A 1-bp deletion Lssaw1 contributes to the development heads. Laser-capture microdissection RNA-sequencing showed that LsSAW1 regulates leaf dorsiventrality loss-of-function downregulates expression...
Abstract Lettuce (Lactuca sativa) is one of the most popular vegetables worldwide, and diverse leaf shapes, including wavy leaves, are important commercial traits. In this study, we examined genetics leaves using an F2 segregating population, identified a major QTL controlling leaves. The candidate region contained LsKN1, which has previously been shown to be indispensable for leafy heads in lettuce. Complementation tests knockout experiments verified function LsKN1 producing LsKN1∇ allele,...
Tamoxifen is commonly used to treat estrogen receptor-positive breast cancer and hepatocellular carcinoma. Phytoconstituents are considered candidates for chemopreventive drugs in treatment. However, it remains unknown what would happen if tamoxifen phytoconstituents were administrated simultaneously. We aimed observe the synergistic antitumor effects of naringenin/quercetin on human hepatic carcinoma explore potential underlying molecular mechanisms. The HepG2 cell line was as an vitro...
Abstract Background Isatidis Radix, the root of Isatis indigotica Fort. (Chinese woad) can produce a variety efficacious compound with medicinal properties. The tetraploid I. plants exhibit superior phenotypic traits, such as greater yield, higher bioactive compounds accumulation and enhanced stress tolerance. In this study, comparative transcriptomic metabolomic study on Radix autotetraploid its progenitor was performed. Results Through targeted metabolic profiling, 283 metabolites were...
Soil salt-alkalization is a common yet critical environmental stress factor for plant growth and development. Discovering exploiting genes associated with alkaline tolerance in maize ( Zea mays L.) helpful improving resistance. Here, an association panel consisting of 200 lines was used to identify the genetic loci responsible tolerance-related traits seedlings. A total nine single-nucleotide polymorphisms (SNPs) their candidate were found be significantly using genome-wide study (GWAS). An...
Background: Dendrobium officinale is a perennial epiphytic herb in Orchidaceae. Cultivated products are the main alternative for clinical application due to shortage of wild resources. However, phenotype and quality D. have changed post-artificial cultivation, environmental cues such as light, temperature, water, nutrition supply major influencing factors. This study aims unveil mechanisms beneath cultivation-induced variation by analyzing changes metabolome transcriptome seedlings treated...
We identified the loss of BoFLC gene as cause non-vernalization requirement in B. oleracea. Our developed codominant marker can be used for breeding program oleracea crops. Many species Brassicaceae family, including some Brassica crops, require vernalization to avoid pre-winter flowering. Vernalization is an unfavorable trait Chinese kale (Brassica var. chinensis Lei), a stem vegetable, and therefore it has been lost during its domestication/breeding process. To reveal genetics variation,...
Algae are ubiquitous in eutrophic surface waters and they play important roles the sunlight-driven transformation of environmental contaminants. The present study investigated their role photodegradation tetracycline. results showed that algal organic matter, both extracellular intracellular promote tetracycline photodegradation, but different ways performances. Extracellular matter exerts greater photolysis effectiveness than ones with logarithmic phase more effective from algae's declining...
As the fifth most common cancer and second leading cause of related deaths worldwide, hepatocellular carcinoma (HCC) is an aggressive tumor type with poor prognosis causing 250,000 to one million annually. Alcoholic steatohepatitis (ASH) non-alcoholic (NASH) are two major growing risk factors both may develop liver fibrosis or even HCC. However, compared rate patients ASH progressing HCC annually, it much lower in NASH patients. The present study clarify protein expression epigenetic...
Objective: To investigate the effect of Silent information regulator 1 (Sirt1) on expression profile long non-coding RNA (lncRNA) in macrophages upon lipopolysaccharide (LPS) treatment. Methods: Peritoneal (PM) were isolated from nine wild-type C57BL/6 male mice (wild-type group) and myeloid-specific Sirt1 knock-out (knock-out group). samples extracted stimulated with μg/ml LPS. Sequencing differentially expressed lncRNA screened after was quantified. The threshold set for up-and...