A5085137854- Prenatal Screening and Diagnostics
- Antioxidant Activity and Oxidative Stress
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Lipoproteins and Cardiovascular Health
- Cardiovascular Health and Disease Prevention
- Parvovirus B19 Infection Studies
- Nitric Oxide and Endothelin Effects
- Blood groups and transfusion
- Fetal and Pediatric Neurological Disorders
- Medical and Biological Sciences
- Oral and Maxillofacial Pathology
- Salivary Gland Tumors Diagnosis and Treatment
- Advanced Glycation End Products research
- Pregnancy and preeclampsia studies
- Cancer Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Tryptophan and brain disorders
- Diet, Metabolism, and Disease
- dental development and anomalies
- Oral Health Pathology and Treatment
- Oral and gingival health research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ethics and Legal Issues in Pediatric Healthcare
- Stress Responses and Cortisol
University Medical Center Utrecht
2012-2025
Wilhelmina Children's Hospital
2011-2025
Utrecht University
2012-2025
Amsterdam University Medical Centers
2024
University of Guelph
2019
Amsterdam UMC Location Vrije Universiteit Amsterdam
2007-2017
S.P.E.C.I.E.S.
2017
Procter & Gamble (Germany)
2017
University Medical Center
2008-2016
University Hospital and Clinics
2008-2016
Reduced bioavailability of endogenous nitric oxide (NO) is a central pathophysiological event in hypertension and other cardiovascular diseases. Recently, it was demonstrated that inorganic nitrate from dietary sources converted vivo to form nitrite, NO, bioactive nitrogen oxides. We tested the hypothesis supplementation may have therapeutic effects model renal disease. Sprague–Dawley rats subjected unilateral nephrectomy chronic high-salt diet 3 weeks age developed hypertension, cardiac...
Summary objective Sex differences are found in most components of the insulin resistance syndrome and associated cardiovascular risk profile. These attributed to sex‐specific sex steroid profiles, but effects steroids on individual remain incompletely understood. design Prospective, intervention study. subjects In 37 young (age range 16–36 years), nonobese [body mass index (BMI) < 29], transsexual subjects, ethinyl oestradiol (100 µg/day) + cyproterone acetate mg/day) administration were...
Abstract. Objectives. The UK Prospective Diabetes Study (UKPDS) showed that treatment with metformin decreases macrovascular morbidity and mortality independent of glycaemic control. We hypothesized may achieve this by improving endothelial function chronic, low‐grade inflammation. Data on issue are scarce we therefore tested, in the setting a randomized, placebo‐controlled trial, whether can affect Design. Hyperinsulinaemia Outcome its Metabolic Effects (HOME) trial is double‐blind which...
The reactive advanced glycation end product (AGE) precursor methylglyoxal (MGO) and MGO-derived AGEs are associated with diabetic vascular complications also an increase in oxidative stress. Glyoxalase-I (GLO-I) transgenic rats were used to explore whether overexpression of this MGO detoxifying enzyme reduces levels stress a rat model diabetes. Rats made streptozotocin, after 12 weeks, plasma multiple tissues isolated for analysis AGEs, carbonyl stress, GLO-I activity was significantly...
To evaluate the diagnostic performance of noninvasive fetal blood group genotyping.Descriptive analysis.Dutch national reference laboratory for pregnancies complicated by alloimmunisation.All consecutive alloimmunised pregnant women whom genotyping rhesus D, c, E or K in maternal plasma was performed from 2003 up to 2010.The test results each individual assay were collected. Real-time polymerase chain reaction RHD exon 5 and 7, specific allele RHCE KEL gene. A stringent algorithm applied. In...
Background. During haemodialysis (HD), polymorphonuclear cells (PMNs) and platelets are activated release various granule products, including myeloperoxidase (MPO) platelet factor 4 (PF4). MPO triggers the generation of reactive oxygen species, leading to irreversible protein, carbohydrate lipid modification. PF4 probably also contributes oxidative stress. As previously shown, HD-induced PMN degranulation is almost completely abolished during citrate anticoagulation, most due its calcium...
<h3>Background</h3> Patients with chronic kidney disease have an increased risk of cardiovascular disease. Oxidative stress has been proposed to play a role in the development among these patients. <h3>Methods</h3> We conducted randomized, double-blind trial 93 patients (Cockcroft-Gault equation: creatinine clearance, 38 ± 15 [mean SD] mL/min per 1.73 m<sup>2</sup>[0.63 0.25 mL/s m<sup>2</sup>]) investigate effect treatment strategy designed primarily achieve stepwise oxidative reduction on...
In Brief OBJECTIVE: To determine the diagnostic accuracy of noninvasive fetal sex determination in maternal plasma. METHODS: All consecutive patients for whom plasma was performed our laboratory from 2003 up to 2009 were included study. Real-time polymerase chain reaction SRY gene and multicopy DYS14 marker sequence. A stringent algorithm applied. case a positive result both Y chromosome–specific assays, male-bearing pregnancy reported. negative result, presence DNA ascertained through use...
Vanishing white matter disease is a genetic leukoencephalopathy caused by mutations in eukaryotic translation initiation factor 2B. Patients experience slowly progressive neurological deterioration with episodes of rapid clinical worsening triggered stress.The may occur at any age and leads to early death.Characteristic neuropathological findings include cystic degeneration the feeble, if any, reactive gliosis, dysmorphic astrocytes paucity myelin despite an increase oligodendrocytic...
<b>Objective</b> To determine the accuracy of non-invasive fetal testing for <i>RHD</i> gene in week 27 pregnancy as part an antenatal screening programme to restrict anti-D immunoglobulin use women carrying a child positive <i>RHD</i>. <b>Design</b> Prospectively monitoring compared with serological cord blood typing on introduction test. Fetal was performed duplex real time quantitative polymerase chain reaction, cell-free DNA isolated from 1 mL maternal plasma The study period...
Please cite this paper as: Scheffer P, Ait Soussan A, Verhagen O, Page-Christiaens G, Oepkes D, de Haas M, van der Schoot C. Noninvasive fetal genotyping of human platelet antigen-1a. BJOG 2011;118:1392–1395. We describe a reliable noninvasive antigen (HPA)-1a assay on real-time polymerase chain reaction (PCR) platform using cell-free DNA isolated from maternal blood. Nonspecific amplification is overcome by pre-PCR digestion the with Msp1 restriction enzyme. HPA-1a offers safe method for...
Background Endothelial dysfunction (ED), low-grade inflammation (LGI) and oxidative stress (OxS) may be involved in the pathobiology of depression. Previous studies on association these processes depression have yielded contradictory results. We therefore investigated comprehensively, a population-based cohort study, between ED, LGI OxS one hand depressive symptoms other. Method used data from Hoorn Study determined biomarkers ED [flow-mediated dilatation (FMD), von Willebrand factor,...
Background Oxidative stress has been implicated in the pathophysiology of major depressive disorder (MDD) and anxiety disorders may be influenced by antidepressant use. This study investigated association oxidative stress, measured plasma levels F2-isoprostanes 8-hydroxy-2′-deoxyguanosine (8-OHdG) reflecting lipid DNA damage respectively, with MDD, use a large cohort. Method Data was derived from Netherlands Study Depression Anxiety including patients current ( N = 1619) or remitted 610) MDD...
Background. Oxidative stress is increasingly important in health research. Therefore, it necessary to understand which factors determine basal oxidative stress. This study examines the associations of various determinants with markers DNA and lipid damage: 8-hydroxy-2'-deoxyguanosine (8-OHdG) F2-isoprostanes. Methods. Data are from Netherlands Study Depression Anxiety; 1117 subjects (18-65 years) without a current psychiatric diagnosis. Multivariable regression analyses were conducted plasma...
Noninvasive prenatal testing by cell-free DNA analysis is offered to pregnant women worldwide screen for fetal aneuploidies. In noninvasive testing, the fraction of in maternal circulation measured as a quality control parameter. Given that originates from placenta, might also reflect placental health and pregnancy adaptation.
Plasma levels of liver transaminases, including alanine aminotransferase (ALT), are elevated in most cases nonalcoholic fatty disease (NAFLD). Elevated ALT associated with insulin resistance, and subjects NAFLD have features the metabolic syndrome that confer high-risk cardiovascular disease. Alanine predicts development type 2 diabetes (DM2) syndrome. However, role overt DM2 has yet not been explored.In a cross-sectional study, 64 normotriglyceridaemic were studied regard to relation...