A5050381914- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Listeria monocytogenes in Food Safety
- Neurogenesis and neuroplasticity mechanisms
- Microbial Community Ecology and Physiology
- Microbial Natural Products and Biosynthesis
- Cancer Research and Treatments
- Streptococcal Infections and Treatments
- Signaling Pathways in Disease
- Consumer Perception and Purchasing Behavior
- Antifungal resistance and susceptibility
- Vibrio bacteria research studies
- Diverse Topics in Contemporary Research
- Cultural and Historical Studies
- Cancer therapeutics and mechanisms
- Salmonella and Campylobacter epidemiology
- Neuroscience and Neuropharmacology Research
University of Wisconsin–Madison
2020-2022
Oregon State University
2016-2021
Human induced pluripotent stem cells (hiPSCs) show great promise in the development of novel strategies to mitigate reproductive diseases and promote successful outcomes. Recently, a approach for fast efficient differentiation ovarian support (OSCs) generate versatile platform basic research clinical applications was demonstrated. This study details process application an OSC product, known as Fertilo, improve vitro maturation (IVM) human oocytes, method referred OSC-IVM. First,...
Pathogenic bacteria rely on protein phosphorylation to adapt quickly stress, including that imposed by the host during infection. Penicillin-binding and serine/threonine-associated (PASTA) kinases are signal transduction systems sense cell wall integrity modulate multiple facets of bacterial physiology in response envelope stress. The PASTA kinase cytosolic pathogen Listeria monocytogenes, PrkA, is required for stress responses, survival, virulence, yet its substrates downstream signaling...
Increased usage of daptomycin (DAP) for methicillin-resistant Staphylococcus aureus (MRSA) infections has led to emergence DAP-resistant (DAP-R) strains, resulting in treatment failures.
Daptomycin-nonsusceptible (DAP-NS) Staphylococcus aureus often exhibits gain-in-function mutations in the mprF gene (involved positive surface charge maintenance). Standard β-lactams, although relatively inactive against methicillin-resistant S. (MRSA), may prevent emergence of and DAP-NS. We determined if β-lactams might also impact DAP-NS isolates already possessing an mutation to revert them DAP-susceptible (DAP-S) phenotypes and, so, whether this is associated with specific...
Methicillin-resistant Staphylococcus aureus (MRSA) is a serious clinical threat due to innate virulence properties, high infection rates, and the ability develop resistance multiple antibiotics, including lipopeptide daptomycin (DAP). The acquisition of DAP (DAP-R) in MRSA has been linked with several characteristic alterations cell envelope.
The reversal of daptomycin resistance in MRSA to a daptomycin-susceptible phenotype following prolonged passage selected β-lactams occurs coincident with the accumulation multiple point mutations mprF gene. MprF regulates surface charge by modulating content and translocation positively charged cell membrane phospholipid, lysyl-phosphatidylglycerol (LPG). precise adaptations accompanying such β-lactam-induced perturbations are unknown. This study examined key metrics relevant antimicrobial...
Abstract From arid, high desert soil samples collected near Bend, Oregon, 19 unique bacteria were isolated. Each strain was identified by 16S rRNA gene sequencing, and their organic extracts tested for antibacterial antiproliferative activities. Noteworthy, six (30 %) exhibited strong inhibition resulting in less than 50 % cell proliferation more one cancer model, at 10 μg/mL. Principal component analysis (PCA) of LC/MS data revealed drastic differences the metabolic profiles found these...
Clinical treatment options for daptomycin (DAP)-resistant (DAP-R), methicillin-resistant Staphylococcus aureus (MRSA) infections are relatively limited. Current therapeutic strategies often take advantage of potential synergistic activity DAP plus β-lactams; however, the mechanisms underlying their combinatorial efficacy likely complex and remain incompletely understood.
ABSTRACT Pathogenic bacteria rely on protein phosphorylation to adapt quickly stress, including that imposed by the host during infection. Penicillin-binding and serine/threonine- associated (PASTA) kinases are signal transduction systems sense cell wall integrity modulate multiple facets of bacterial physiology in response envelope stress. The PASTA kinase cytosolic pathogen Listeria monocytogenes , PrkA, is required for stress responses, survival, virulence, yet its substrates downstream...
Over the time frame from around 1940 s to end of 2014, 175 small molecules were approved as anticancer agents. Of those, 49% either natural products or directly derived therefrom [1]. Thus, still represent a promising new source for continued drug development and discovery. Soil bacteria specifically are responsible number drugs used clinically today, such doxorubicin mitomycin c. Herein, 17 soil isolated Bend, Oregon tested cytotoxic activity against colon, breast, prostate cancer model...