Noradrenaline and serotonin are known to control arginine-vasopressin (AVP) oxytocin (OT) secretion in the systemic circulation. The aim of current study was investigate whether these monoamines also able influence AVP OT expression paraventricular (PVN) supraoptic nuclei (SON). To test this hypothesis, we used Tg8 transgenic mice KO for monoamine oxidase-A gene, which present high levels noradrenaline brain. were evaluated at peptide mRNA by immunohistochemistry, enzyme immunoassay, <i>in...

Experimental allergic encephalomyelitis (EAE) was induced in SJL/J mice by adoptive transfer of MBP-reactive T cells order to investigate the role astrocytes pathology. GFAP protein and mRNA expression (analyzed using semi-quantitative Western blot RT-PCR techniques) were upregulated spinal cord mice, which had developed a complete paralysis hind- fore-limbs tail (grade 4 EAE), thus establishing that reactive gliosis occurred under these experimental conditions. Within same samples similar...

MAGED1, NECDIN and MAGEL2 are members of the MAGE gene family. The latter two these genes have been involved in Prader–Willi syndrome (PWS), which includes hyperphagia, repetitive compulsive behaviors, cognitive impairment. Here, we show that Maged1-deficient mice develop progressive obesity associated with hyperphagia reduced motor activity. Loss Maged1 also results a complex behavioral social interactions memory, deficient sexual behavior, as well increased anxiety self-grooming. Oxytocin...

Bisphenol A (BPA) is a widespread estrogenic compound. We investigated the effects of maternal exposure to BPA at reference doses on sexual behavior and neuroendocrine functions female offspring in C57BL/6J mice. The dams were orally exposed vehicle alone or vehicle-containing equivalent no observed adverse effect level (5 mg/kg body weight per day) tolerable daily intake (TDI, 0.05 from gestational day 15 until weaning. Developmental increased lordosis quotient naive females TDI dose only....

Background: Courtship behavior plays a critical role in attracting females and reproduction success. However, the effects of exposure to ubiquitous contaminant di(2-ethylhexyl) phthalate (DEHP) on these behaviors and, particular, courtship vocalizations have not been examined. Objective: The adult DEHP mating gonadotropic axis neural mechanisms involved DEHP-induced were analyzed male mice. Methods: Adult C57BL/6J males orally exposed (0, 0.5, 5, 50μg/kg/d) for 4 wk. Olfactory preference,...

Ovarian oestradiol is essential for pubertal maturation and adult physiology of the female reproductive axis. It acts at central peripheral sites through two main oestrogen receptors (ER) α β. Here we investigate role ERβ on effects oestradiol, by generating a mouse line specifically lacking gene in neuronal glial cells. Central deletion delays age vaginal opening first oestrous reduces uterine weight without affecting body growth. Analysis factors necessary progression shows reduced levels...

Estrogen receptor (ER) α is involved in several estrogen-modulated neural and peripheral functions. To determine its role the expression of female male reproductive behavior, a mouse line lacking ERα nervous system was generated. Mutant females did not exhibit sexual behavior despite normal olfactory preference, had reduced number progesterone receptor-immunoreactive neurons ventromedial hypothalamus. males displayed moderately impaired unaffected fertility, evidences altered organization...

Estradiol derived from neural aromatization of testosterone plays a key role in the organization and activation structures underlying male behaviors. This study evaluated contribution estrogen receptor (ER) β estradiol-induced modulation social mood-related behaviors by using mice lacking ERβ gene nervous system. Mutant males exhibited reduced interaction with same-sex congeners impaired aggressive behavior. They also displayed increased locomotor activity, or unaffected anxiety-state level...

Abstract An increasing number of neurologic diseases is associated with autoimmunity. The immune effectors contributing to the pathogenesis such are often unclear. To explore whether self-reactive CD8 T cells could attack CNS neurons in vivo, we generated a mouse model which influenza virus hemagglutinin (HA) expressed specifically neurons. Transfer cytotoxic anti-HA induced an acute but reversible encephalomyelitis HA-expressing recipient mice. Unexpectedly, diabetes insipidus developed...

We studied the role of testosterone, mediated by androgen receptor (AR), in modulating temporal order memory for visual objects. For this purpose, we used male mice lacking AR specifically nervous system. Control and mutant males were gonadectomized at adulthood supplemented with equivalent amounts testosterone to normalize their hormonal levels. found that neural deletion selectively impaired processing information objects, without affecting classical object recognition or anxiety-like...

Abstract Nitric oxide (NO) is known to regulate the release of arginine‐vasopressin (AVP) and oxytocin (OT) by paraventricular nucleus (PVN) supraoptic (SON). The aim current study was identify in these nuclei NO‐producing neurons NO‐receptive cells mice. determination NO‐synthesizing performed double immunohistochemistry for neuronal form NO synthase (NOS), AVP or OT. Besides, we visualized detecting cyclic GMP (cGMP), major second messenger NO, on hypothalamus slices. Neuronal NOS...

Motoneurons in the spinal nucleus of bulbocavernosus (SNB) and their target (BC) levator ani (LA) muscles play a role male copulation fertility. Testosterone (T) induces sexual differentiation this SNB neuromuscular system during development maintains its activation adulthood. In rat, T-induced effects mostly involve androgen receptor (AR). However, central AR remains to be studied with pertinent genetic models. We addressed question by using specific motoneuron immunolabeling retrograde...

Sexual experience in male rodents, induced by a first exposure to receptive female, improves efficiency of following copulations. In mice, the mechanisms supporting this improvement are poorly understood. We characterized molecular modifications mouse hypothalamic medial preoptic area (mPOA), main integrative structure for sexual behaviour, after single mating event. This paradigm long-lasting behavioural improvements and mPOA morphological changes, evidenced dendritic spine maturation an...

Abstract Magnocellular neurons of the hypothalamic supraoptic nuclei (SON) are involved in synthesis and release two major neuropeptides: oxytocin (OT) arginine‐vassopressin (AVP). Neurochemical plasticity this system is induced by physiological conditions such as lactation, parturition dehydration, may be accompanied reversible structural affecting neurons, astrocytes extracellular matrix (ECM). The noradrenergic plays a critical role triggering chemical associated with plasticity. Matrix...

Free radicals, or reactive oxygen species (ROS), are highly byproducts of degradation. They well known for their cellular toxicity, but few studies have analyzed potential role in homeostatic processes. We investigated ROS production and function during the arginine vasopressin (AVP) hypothalamic response to hyperosmolarity. Six-week-old male C3H/HeJ mice were subjected salt loading 2 8 d. The osmotic axis was progressively activated reached a new steady-state status at d as demonstrated by...

DP71 is the major cerebral dystrophin isoform and exerts its multiple functions via dystrophin-associated protein complex (DAPC), also comprised of β-dystroglycan (β-DG) α1-syntrophin (α1-Syn). Since disruption leads to impairment in central control osmoregulatory axis, we investigated: 1) DAPC composition hypothalamic supraoptic nucleus (SON) paraventricular (PVN) Dp71-null mice; 2) expression activity neuronal nitric oxide synthase (nNOS), because it a potential partner functional index...

Over the years, advances in immunohistochemistry techniques have been a critical step detecting and mapping neuromodulatory substances central nervous system. The better quality specificity of primary antibodies, new staining procedures spectacular development imaging technologies allowed such progress. Very recently, methods permitting tissue transparency successfully used on brain tissues. In present study, we combined whole-mount immunostaining for tyrosine hydroxylase (TH), oxytocin...

Patients with Duchenne muscular dystrophy (DMD) and mdx mice, devoid of dystrophin proteins, show altered ionic homeostasis. To clarify dystrophin's involvement in the central control osmotic stimuli, we investigated effect disruption Dp71, major form brain, on hypothalamoneurohypophysis system (HNHS) osmoregulatory response. Dp71 Dp140 are principal DMD gene products supraoptic nucleus (SON) neurohypophysis (NH). They present astrocyte pituicyte end-feet, suggesting both intrinsic...