A5026880214- Neuroendocrine regulation and behavior
- Sexual function and dysfunction studies
- Hypothalamic control of reproductive hormones
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neurobiology and Insect Physiology Research
- Animal Behavior and Reproduction
- Neural dynamics and brain function
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Adolescent Sexual and Reproductive Health
- Photoreceptor and optogenetics research
- Neuroscience of respiration and sleep
- Biochemical Analysis and Sensing Techniques
- Memory and Neural Mechanisms
- Regulation of Appetite and Obesity
- Sleep and Wakefulness Research
- Complex Systems and Decision Making
- Behavioral and Psychological Studies
- Image Processing Techniques and Applications
- Single-cell and spatial transcriptomics
- Sexual Differentiation and Disorders
University of Washington
2022-2025
Washington Center
2022-2024
University of Maryland, Baltimore
2020-2021
National Institute of Diabetes and Digestive and Kidney Diseases
2018-2019
National Institutes of Health
2018-2019
National Institute on Drug Abuse
2018-2019
Chemogenetic tools such as designer receptors exclusively activated by drugs (DREADDs) are routinely used to modulate neuronal and non-neuronal signaling activity in a relatively noninvasive manner. The first generation of DREADDs were templated from the human muscarinic acetylcholine receptor family insensitive endogenous agonist but instead clozapine-N-oxide (CNO). Despite undisputed success CNO an activator DREADDs, it has been known for some time that is subject low rate metabolic...
When presented with a choice, organisms need to assimilate internal information external stimuli and past experiences rapidly flexibly optimize decisions on moment-to-moment basis. We hypothesized that increasing hunger intensity would curb expression of social behaviors such as mating or territorial aggression; we further interactions, reciprocally, influence food consumption. assessed competition between these motivations from both perspectives mice within resident-intruder paradigm. found...
Male ejaculation acutely suppresses sexual motivation in male mice. In contrast, relatively little is known about how affects and behavior female How the brain responds to completion of mating also unclear. Here, by using self-paced assay, we first demonstrate that mice show decreased after experiencing ejaculation. By brain-wide analysis activity-dependent labeling, next pinpointed medial preoptic area as a region strongly activated during post-ejaculatory period. Furthermore, freely moving...
Summary Abstract Spatially integrated mechanisms of consciousness are unclear 1,2 . An approach to manipulate brainwide circuits regulating via synthetic central nervous system activation may pave the way for more precise transitions in and reveal underlying mechanisms. Toward this goal, we leverage anesthesia as a tool probe at cellular resolution within intact network. We perform chemogenetic capture 3,4 isoflurane anesthesia-activated circuitry mice —in parallel with electrocorticography...
Male ejaculation acutely suppresses sexual motivation in male mice. In contrast, relatively little is known about how affects and behavior female How the brain responds to completion of mating also unclear. Here, by using a self-paced assay, we first demonstrate that mice show decreased after experiencing ejaculation. By brain-wide analysis activity-dependent labeling, next pin-pointed medial preoptic area as region strongly activated during post-ejaculatory period. Furthermore, freely...
High-throughput volumetric fluorescent microscopy pipelines can spatially integrate whole-brain structure and function at the foundational level of single cells. However, conventional protein (FP) modifications used to discriminate cells possess limited efficacy or are detrimental cellular health. Here, we introduce a synthetic nondeleterious nuclear localization signal (NLS) tag strategy, called “Arginine-rich NLS” (ArgiNLS), that optimizes genetic labeling downstream image segmentation by...
Abstract Individuals suffering from substance use disorder often experience relapse events that are attributed to drug craving. Insular cortex (IC) function is implicated in processing drug‐predictive cues and thought be a critical substrate for craving, but the downstream neural circuit effectors of IC mediate reward poorly described. Here, we uncover functional connectivity an projection ventral bed nucleus stria terminalis (vBNST), portion extended amygdala has been previously shown...
Abstract Aggression is an evolutionarily conserved, adaptive component of social behavior. Studies in male mice illustrate that aggression influenced by numerous factors including the degree to which individual finds rewarding and will work for access attack subordinate mice. While such studies have expanded our understanding molecular circuit mechanisms very little known about female aggression, owed part limited availability valid mouse models females. Here we use ethologically relevant...
SUMMARY Male ejaculation acutely suppresses sexual motivation in male mice. In contrast, relatively little is known about how affects and behavior female How the brain responds to completion of mating also unclear. Here, by using self-paced assay, we first demonstrate that mice show decreased after experiencing ejaculation. By brain-wide analysis activity-dependent labeling, next pinpointed medial preoptic area as a region strongly activated during post-ejaculatory period. Furthermore,...
High-throughput volumetric fluorescent microscopy pipelines can spatially integrate whole-brain structure and function at the foundational level of single-cells. However, conventional protein (FP) modifications used to discriminate single-cells possess limited efficacy or are detrimental cellular health. Here, we introduce a synthetic non-deleterious nuclear localization signal (NLS) tag strategy, called 'Arginine-rich NLS' (ArgiNLS), that optimizes genetic labeling downstream image...
The completion of mating acutely suppresses sexual motivation in male mice. In contrast, relatively little is known about how the affects and behavior female How brain responds to also unclear. Here, by using self-paced assay, we first demonstrate that mice show decreased after mating. By brain-wide analysis activity-dependent labeling, next pin-pointed medial preoptic area as a region strongly responding completion. Furthermore, freely moving vivo calcium imaging compare neural activity...
Male ejaculation acutely suppresses sexual motivation in male mice. In contrast, relatively little is known about how affects and behavior female How the brain responds to completion of mating also unclear. Here, by using a self-paced assay, we first demonstrate that mice show decreased after experiencing ejaculation. By brain-wide analysis activity-dependent labeling, next pin-pointed medial preoptic area as region strongly activated during post-ejaculatory period. Furthermore, freely...
Abstract Background A key challenge in developing new treatments for neuropsychiatric illness is the disconnect between preclinical models and complexity of human social behavior. We aimed to integrate voluntary self-administration into a rodent stress model, as platform identification basic brain behavior mechanisms underlying stress-induced individual differences motivation. Here, we introduce an operant (OSS) procedure with male female mice, where lever presses are reinforced by freely...