A5070135007- Meningioma and schwannoma management
- Chromatin Remodeling and Cancer
- Glioma Diagnosis and Treatment
- Neurofibromatosis and Schwannoma Cases
- Head and Neck Surgical Oncology
- Connexins and lens biology
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Brain Metastases and Treatment
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebrospinal fluid and hydrocephalus
- Bone Tumor Diagnosis and Treatments
- Ocular Oncology and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Ear and Head Tumors
- Spinal Fractures and Fixation Techniques
- Neurosurgical Procedures and Complications
- Intracranial Aneurysms: Treatment and Complications
- Adrenal and Paraganglionic Tumors
- IgG4-Related and Inflammatory Diseases
- Microtubule and mitosis dynamics
- Vascular Malformations Diagnosis and Treatment
- Spinal Dysraphism and Malformations
- Neuroblastoma Research and Treatments
Universidad de Salamanca
2012-2024
Instituto de Investigación Biomédica de Salamanca
2013-2024
Complejo Hospitalario de Salamanca
2008-2023
Junta de Castilla y León
2021
Instituto de Estudios de Ciencias de la Salud de Castilla y León
2014
University of Coimbra
2014
Center for Cancer Research
2014
Tumor recurrence remains the major clinical complication of meningiomas, majority recurrences occurring among WHO grade I/benign tumors. In present study, we propose a new scoring system for prognostic stratification meningioma patients based on analysis large series meningiomas followed median >5 years. cytogenetics were systematically investigated by interphase fluorescence in situ hybridization 302 samples, and proposed classification was further validated an independent cases (n = 132)...
The distribution and role of tumor-infiltrating leucocytes in glioblastoma (GBM) remain largely unknown. Here, we investigated the cellular composition 55 primary (adult) GBM samples by flow cytometry correlated tumor immune profile with patient features at diagnosis outcome. single-cell suspensions were stained (n = 44) recurrence following radiotherapy chemotherapy 11) a panel 8-color monoclonal antibody combinations for identification enumeration (GFAP+ CD45- ) normal astrocytic cells,...
Rhabdoid meningiomas (RM) shows heterogeneous histological findings, and a wide variety of chromosomal copy number alterations (CNA) are associated with an unpredictable course the disease. In this study, we analyzed series 305 RM samples from patients previously reported in literature 33 23 studied our laboratory. Monosomy 22-involving minimal but most common recurrent region loss 22q11.23 was observed alteration, followed by losses chromosomes 14, 1, 6, 19, polysomies 17, 1q, 20, gains...
Meningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between number immunophenotype inflammatory cells tumor as evaluated by multiparameter flow cytometry, clinico-biological, cytogenetic gene expression profile (GEP) 75 meningioma patients. Overall, our results showed a close amount cellular composition cell infiltrates tumors. Notably, tumors with isolated monosomy 22/del(22q)...
Meningioma was the first solid tumor shown to contain a recurrent genetic alteration e.g. monosomy 22/del(22q), NF2 being most relevant gene involved. Although 22/del(22q) is present in half of all meningiomas, and meningiomas frequently carry mutations, no study has been reported so far which both alterations are simultaneously assessed correlated with features disease.Here, we analyzed frequency copy number changes involving chromosome 22 mutations 20 sporadic using high-density...
Background: The prognostic impact of the expression profile genes recurrently amplified in glioblastoma multiforme (GBM) remains controversial. Methods: We investigated RNA gene epidermal growth factor receptor (EGFR), cyclin-dependent kinase 4 (CDK4), murine doble minute (MDM4), and platelet derived alpha (PDGFRA) 83 primary GBM tumors vs. 42 normal brain tissue samples. Interphase FISH (iFISH) analysis for four genes, together with intragenic deletions EGFR PDGFRA, were evaluated parallel...
Abstract Despite recent advances in the identification of cytogenetic profiles meningiomas, a significant group tumors still show normal karyotypes or few chromosomal changes. The authors analyzed profile 50 meningiomas using fluorescence situ hybridization and high‐density (500 K) single nucleotide polymorphism (SNP) arrays. Our results confirm that del(22q) (52%) del(1p) (16%) (common deleted regions: 22q11.21‐22q13.3. 1p31.2‐p36.33) are most frequent alterations. Additionally, recurrent...
Limited information exists about the impact of cytogenetic alterations on protein expression profiles individual meningioma cells and their association with clinicohistopathological characteristics disease. The aim this study is to investigate potential between immunophenotypic profile single most relevant features tumour.Multiparameter flow cytometry (MFC) was used evaluate tumour (n = 51 patients) Affymetrix U133A chip applied for analysis gene 40) samples, cytogenetically characterized by...
Abstract Diagnosis and classification of gliomas mostly relies on histopathology a few genetic markers. Here we interrogated microarray gene expression profiles (GEP) 268 diffuse astrocytic gliomas—33 astrocytomas (DA), 52 anaplastic (AA) 183 primary glioblastoma (GBM)—based multivariate analysis, to identify discriminatory GEP that might support precise histopathological tumor stratification, particularly among inconclusive cases with II–III grade diagnosed, which have different prognosis...
Growth hormone (GH)-secreting pituitary tumors (GHomas) are the most common acromegaly cause. At diagnosis, of them macroadenomas, and up to 56% display cavernous sinus invasion. Biomarker assessment associated with tumor growth invasion is important optimize their management.
Human WHO grade 1 meningiomas are generally considered benign tumors; despite this, they account for ≈50% of all recurrent meningiomas. Currently, limited data exist about the mutational profiles and patient outcome. We investigated genetic variants present in 32 using whole exome sequencing, correlated gene with tumor cytogenetics Overall, harbored numerous heterogeneous variants, which most frequently affected NF2 (47%) to a less extent PNMA6A (22%), TIGD1 (16%), SMO (13%), PTEN CREG2...
// María González-Tablas 1 , Inês Crespo 2, 3 Ana Luísa Vital Álvaro Otero 4 Belén Nieto 5 Pablo Sousa Carmen Patino-Alonso Luis Antonio Corchete 6 Hermínio Tão 7 Olinda Rebelo 8 Marcos Barbosa 7, 9 Maria Rosário Almeida 2 Filipa Guedes Celeste Lopes Pim J. French 10 Alberto Orfao 1, 11, * and Dolores Tabernero Centre for Cancer Research (CIC IBMCC-CSIC/USAL), Department of Medicine, CIBERONC, University Salamanca, Spain Neuroscience Cell Biology, Coimbra, Portugal Faculty Pharmacy, Servicio...
We report a divergent ependymal tumor of the posterior fossa (ependymoblastoma/anaplastic ependymoma) observed in an 8-year-old boy. The showed histological pattern typical ependymoblastoma (tubular-papillary fetaloid architecture with stratification cells) next to areas which findings anaplastic ependymoma were detected. immunohistochemical study confirmed our diagnostic suspicion, allowing us establish differential diagnosis other entities such as medulloblastoma, medulloepithelioma,...
Rhabdoid meningiomas (RM) are a rare meningioma subtype with heterogeneous clinical course which is more frequently associated recurrence, even among tumors undergoing-complete surgical removal. Here, we retrospectively analyzed the clinical-histopathological and cytogenetic features of 29 tumors, from patients recurrent (seven primary 14 tumors) vs. non-recurrent RM (n = 8). Recurrent showed one (29%), two (29%) or three (42%) recurrences. BAP1 loss expression was found in third all at...